First published online February 19, 2004; 10.1104/pp.103.035782
Plant Physiology 134:1006-1016 (2004)
© 2004 American Society of Plant Biologists
ENVIRONMENTAL STRESS AND ADAPTATION
The Arabidopsis Cytosolic Thioredoxin h5 Gene Induction by Oxidative Stress and Its W-Box-Mediated Response to Pathogen Elicitor1
Christophe Laloi2,
Dominique Mestres-Ortega,
Yves Marco,
Yves Meyer and
Jean-Philippe Reichheld*
Laboratoire Génome et Développement des Plantes, Université de Perpignan, Unité Mixte Recherche Centre National de la Recherche Scientifique 5096, 66860 Perpignan, France (C.L., D.M.-O., Y.Me., J.-P.R.); Laboratoire de Biologie Moléculaire des Relations Plantes-Microorganismes, Centre National de la Recherche Scientifique-Institut National de la Recherche Agronomique, Auzeville, Castanet-Tolosan, France (Y.Ma.)
The AtTRXh5 protein belongs to the cytosolic thioredoxins h family that, in Arabidopsis, contains eight members showing very distinct patterns and levels of expression. Here, we show that the AtTRXh5 gene is up-regulated during wounding, abscission, and senescence, as well as during incompatible interactions with the bacterial pathogen Pseudomonas syringae. By electrophoretic mobility shift assays, a binding activity on a W-box in the AtTRXh5 promoter region was found induced by treatments with the P. syringae-derived elicitor peptide flg22, suggesting that a WRKY transcription factor controls AtTRXh5 induction upon elicitor treatment. Remarkably, AtTRXh5 was up-regulated in plants overexpressing WRKY6. More generally, AtTRXh5 is induced in response to oxidative stress conditions. Collectively, our data indicate a possible implication of the cytosolic thioredoxin AtTRXh5 in response to pathogens and to oxidative stresses. In addition, this regulation is unique to AtTRXh5 among the thioredoxin h family, arguing in favor of a speciation rather than to a redundancy of the members of this multigenic family.
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.103.035782.
1 C.L. was the recipient of a fellowship from the French Ministère de la Recherche et de la Technologie.
2 Present address: Institute of Plant Sciences, Plant Genetics, Swiss Federal Institute of Technology (ETH), Universitätsstrasse 2, CH–8092 Zürich, Switzerland.
* Corresponding author; e-mail jpr{at}univ-perp.fr; fax 33–4–68–66–84–99.
Received November 10, 2003;
returned for revision November 23, 2003;
accepted November 23, 2003.
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