This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via ISI Web of Science (28) Citing Articles via Google Scholar Google Scholar Articles by Castillo, M. C. Articles by León, J. Search for Related Content PubMed PubMed Citation Articles by Castillo, M. C. Articles by León, J. Agricola Articles by Castillo, M. C. Articles by León, J.

BIOCHEMICAL PROCESSES AND MACROMOLECULAR STRUCTURES

Gene-Specific Involvement of -Oxidation in Wound-Activated Responses in Arabidopsis¹

Instituto de Biología Molecular y Celular de Plantas (CSIC-UPV), 46022 Valencia, Spain (M.C.C., C.M., J.L.); and Department of Biology, Unit of Plant Biology, University of Fribourg, CH–1700 Fribourg, Switzerland (A.B., J.-P.M.)

The coordinated induced expression of -oxidation genes is essential to provide the energy supply for germination and postgerminative development. However, very little is known about other functions of -oxidation in nonreserve organs. We have identified a gene-specific pattern of induced -oxidation gene expression in wounded leaves of Arabidopsis. Mechanical damage triggered the local and systemic induction of only ACX1 among acyl-coenzyme A oxidase (ACX) genes, and KAT2/PED1 among 3-ketoacyl-coenzyme A thiolase (KAT) genes in Arabidopsis. In turn, wounding induced KAT5/PKT2 only systemically. Although most of the -oxidation genes were activated by wound-related factors such as dehydration and abscisic acid, jasmonic acid (JA) induced only ACX1 and KAT5. Reduced expression of ACX1 or KAT2 genes, in transgenic plants expressing their corresponding mRNAs in antisense orientation, correlated with defective wound-activated synthesis of JA and with reduced expression of JA-responsive genes. Induced expression of JA-responsive genes by exogenous application of JA was unaffected in those transgenic plants, suggesting that ACX1 and KAT2 play a major role in driving wound-activated responses by participating in the biosynthesis of JA in wounded Arabidopsis plants.

² These authors contributed equally to the paper.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.104.039925.

^* Corresponding author; e-mail jleon{at}ibmcp.upv.es; fax 34–963877859.

Received January 29, 2004; returned for revision February 22, 2004; accepted February 27, 2004.

This article has been cited by other articles:

				K. Hirano, K. Aya, T. Hobo, H. Sakakibara, M. Kojima, R. A. Shim, Y. Hasegawa, M. Ueguchi-Tanaka, and M. Matsuoka Comprehensive Transcriptome Analysis of Phytohormone Biosynthesis and Signaling Genes in Microspore/Pollen and Tapetum of Rice Plant Cell Physiol., October 1, 2008; 49(10): 1429 - 1450. [Abstract] [Full Text] [PDF]

				C. Tretner, U. Huth, and B. Hause Mechanostimulation of Medicago truncatula leads to enhanced levels of jasmonic acid J. Exp. Bot., July 1, 2008; 59(10): 2847 - 2856. [Abstract] [Full Text] [PDF]

				C. Denoux, R. Galletti, N. Mammarella, S. Gopalan, D. Werck, G. De Lorenzo, S. Ferrari, F. M. Ausubel, and J. Dewdney Activation of Defense Response Pathways by OGs and Flg22 Elicitors in Arabidopsis Seedlings Mol Plant, May 22, 2008; (2008) ssn019v1. [Abstract] [Full Text] [PDF]

				M. C. Castillo and J. Leon Expression of the {beta}-oxidation gene 3-ketoacyl-CoA thiolase 2 (KAT2) is required for the timely onset of natural and dark-induced leaf senescence in Arabidopsis J. Exp. Bot., May 1, 2008; 59(8): 2171 - 2179. [Abstract] [Full Text] [PDF]

				H. S. Chung, A. J.K. Koo, X. Gao, S. Jayanty, B. Thines, A. D. Jones, and G. A. Howe Regulation and Function of Arabidopsis JASMONATE ZIM-Domain Genes in Response to Wounding and Herbivory Plant Physiology, March 1, 2008; 146(3): 952 - 964. [Abstract] [Full Text] [PDF]

				L. Kienow, K. Schneider, M. Bartsch, H.-P. Stuible, H. Weng, O. Miersch, C. Wasternack, and E. Kombrink Jasmonates meet fatty acids: functional analysis of a new acyl-coenzyme A synthetase family from Arabidopsis thaliana J. Exp. Bot., February 10, 2008; (2008) erm325v1. [Abstract] [Full Text] [PDF]

				S. Footitt, J. E. Cornah, I. Pracharoenwattana, J. H. Bryce, and S. M. Smith The Arabidopsis 3-ketoacyl-CoA thiolase-2 (kat2-1) mutant exhibits increased flowering but reduced reproductive success J. Exp. Bot., August 28, 2007; (2007) erm146v1. [Abstract] [Full Text] [PDF]

				P. ten Hoopen, A. Hunger, A. Muller, B. Hause, R. Kramell, C. Wasternack, S. Rosahl, and U. Conrad Immunomodulation of jasmonate to manipulate the wound response J. Exp. Bot., July 1, 2007; 58(10): 2525 - 2535. [Abstract] [Full Text] [PDF]

				S. Footitt, D. Dietrich, A. Fait, A. R. Fernie, M. J. Holdsworth, A. Baker, and F. L. Theodoulou The COMATOSE ATP-Binding Cassette Transporter Is Required for Full Fertility in Arabidopsis Plant Physiology, July 1, 2007; 144(3): 1467 - 1480. [Abstract] [Full Text] [PDF]

				A. L. Schilmiller, A. J.K. Koo, and G. A. Howe Functional Diversification of Acyl-Coenzyme A Oxidases in Jasmonic Acid Biosynthesis and Action Plant Physiology, February 1, 2007; 143(2): 812 - 824. [Abstract] [Full Text] [PDF]

				A. J. K. Koo, H. S. Chung, Y. Kobayashi, and G. A. Howe Identification of a Peroxisomal Acyl-activating Enzyme Involved in the Biosynthesis of Jasmonic Acid in Arabidopsis J. Biol. Chem., November 3, 2006; 281(44): 33511 - 33520. [Abstract] [Full Text] [PDF]

				J. Mackenzie, L. Pedersen, S. Arent, and A. Henriksen Controlling Electron Transfer in Acyl-CoA Oxidases and Dehydrogenases: A STRUCTURAL VIEW J. Biol. Chem., October 13, 2006; 281(41): 31012 - 31020. [Abstract] [Full Text] [PDF]

				S. Goepfert, C. Vidoudez, E. Rezzonico, J. K. Hiltunen, and Y. Poirier Molecular Identification and Characterization of the Arabidopsis {Delta}3,5,{Delta}2,4-Dienoyl-Coenzyme A Isomerase, a Peroxisomal Enzyme Participating in the {beta}-Oxidation Cycle of Unsaturated Fatty Acids Plant Physiology, August 1, 2005; 138(4): 1947 - 1956. [Abstract] [Full Text] [PDF]

				C. Li, A. L. Schilmiller, G. Liu, G. I. Lee, S. Jayanty, C. Sageman, J. Vrebalov, J. J. Giovannoni, K. Yagi, Y. Kobayashi, et al. Role of {beta}-Oxidation in Jasmonate Biosynthesis and Systemic Wound Signaling in Tomato PLANT CELL, March 1, 2005; 17(3): 971 - 986. [Abstract] [Full Text] [PDF]

				F. L. Theodoulou, K. Job, S. P. Slocombe, S. Footitt, M. Holdsworth, A. Baker, T. R. Larson, and I. A. Graham Jasmonic Acid Levels Are Reduced in COMATOSE ATP-Binding Cassette Transporter Mutants. Implications for Transport of Jasmonate Precursors into Peroxisomes Plant Physiology, March 1, 2005; 137(3): 835 - 840. [Full Text] [PDF]