This Article Full Text Full Text (PDF) All Versions of this Article: 135/4/2241    most recent pp.104.041947v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via ISI Web of Science (29) Citing Articles via Google Scholar Google Scholar Articles by Schiltz, S. Articles by Burstin, J. Search for Related Content PubMed PubMed Citation Articles by Schiltz, S. Articles by Burstin, J. Agricola Articles by Schiltz, S. Articles by Burstin, J.

DEVELOPMENT AND HORMONE ACTION

Proteome Reference Maps of Vegetative Tissues in Pea. An Investigation of Nitrogen Mobilization from Leaves during Seed Filling¹

Unité de Génétique et Ecophysiologie des Légumineuses à Graines, Institut National de la Recherche Agronomique, 21065 Dijon cedex, France (S.S., K.G., M.H., J.B.); Proteomic Platform, Unité Mixte de Recherche 320, Institut National de la Recherche Agronomique–Centre National de la Recherche Scientifique–Institut National Agronomique Paris–Grignon, Ferme du Moulon 91190 Gif sur Yvette, France (L.N.); and Unité Mixte de Recherche 5004, Agro–Montpellier–Centre National de la Recherche Scientifique–Institut National de la Recherche Agronomique–Université Montpellier II, 34060 Montpellier cedex 1, France (N.S.)

A proteomic approach was used to analyze protein changes during nitrogen mobilization (N mobilization) from leaves to filling seeds in pea (Pisum sativum). First, proteome reference maps were established for mature leaves and stems. They displayed around 190 Coomassie Blue-stained spots with pIs from 4 to 7. A total of 130 spots were identified by mass spectrometry as corresponding to 80 different proteins implicated in a variety of cellular functions. Although the leaf proteome map contained more abundant spots, corresponding to proteins involved in energy/carbon metabolism, than the stem map, their comparison revealed a highly similar protein profile. Second, the leaf proteome map was used to analyze quantitative variations in leaf proteins during N mobilization. Forty percent of the spots showed significant changes in their relative abundance in the total protein extract. The results confirmed the importance of Rubisco as a source of mobilizable nitrogen, and suggested that in pea leaves the rate of degradation of Rubisco may vary throughout N mobilization. Correlated with the loss of Rubisco was an increase in relative abundance of chloroplastic protease regulatory subunits. Concomitantly, the relative abundance of some proteins related to the photosynthetic apparatus (Rubisco activase, Rubisco-binding proteins) and of several chaperones increased. A role for these proteins in the maintenance of a Rubisco activation state and in the PSII repair during the intense proteolytic activity within the chloroplasts was proposed. Finally, two 14-3-3-like proteins, with a potential regulatory role, displayed differential expression patterns during the massive remobilization of nitrogen.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.104.041947.

^* Corresponding author; e-mail schiltz{at}epoisses.inra.fr; fax 33–3–80–693222.

Received March 2, 2004; returned for revision May 27, 2004; accepted June 11, 2004.

This article has been cited by other articles:

				J. Burstin, P. Marget, M. Huart, A. Moessner, B. Mangin, C. Duchene, B. Desprez, N. Munier-Jolain, and G. Duc Developmental Genes Have Pleiotropic Effects on Plant Morphology and Source Capacity, Eventually Impacting on Seed Protein Content and Productivity in Pea Plant Physiology, June 1, 2007; 144(2): 768 - 781. [Abstract] [Full Text] [PDF]

				G. K. Agrawal and J. J. Thelen Large Scale Identification and Quantitative Profiling of Phosphoproteins Expressed during Seed Filling in Oilseed Rape Mol. Cell. Proteomics, November 1, 2006; 5(11): 2044 - 2059. [Abstract] [Full Text] [PDF]

				W. Albertin, T. Balliau, P. Brabant, A.-M. Chevre, F. Eber, C. Malosse, and H. Thiellement Numerous and Rapid Nonstochastic Modifications of Gene Products in Newly Synthesized Brassica napus Allotetraploids Genetics, June 1, 2006; 173(2): 1101 - 1113. [Abstract] [Full Text] [PDF]

				M. Hajduch, J. E. Casteel, K. E. Hurrelmeyer, Z. Song, G. K. Agrawal, and J. J. Thelen Proteomic Analysis of Seed Filling in Brassica napus. Developmental Characterization of Metabolic Isozymes Using High-Resolution Two-Dimensional Gel Electrophoresis Plant Physiology, May 1, 2006; 141(1): 32 - 46. [Abstract] [Full Text] [PDF]

				J. Boudet, J. Buitink, F. A. Hoekstra, H. Rogniaux, C. Larre, P. Satour, and O. Leprince Comparative Analysis of the Heat Stable Proteome of Radicles of Medicago truncatula Seeds during Germination Identifies Late Embryogenesis Abundant Proteins Associated with Desiccation Tolerance Plant Physiology, April 1, 2006; 140(4): 1418 - 1436. [Abstract] [Full Text] [PDF]

				M. Fujiwara, K. Umemura, T. Kawasaki, and K. Shimamoto Proteomics of Rac GTPase Signaling Reveals Its Predominant Role in Elicitor-Induced Defense Response of Cultured Rice Cells Plant Physiology, February 1, 2006; 140(2): 734 - 745. [Abstract] [Full Text] [PDF]

				L. Cui, N. Veeraraghavan, A. Richter, K. Wall, R. K. Jansen, J. Leebens-Mack, I. Makalowska, and C. W. dePamphilis ChloroplastDB: the Chloroplast Genome Database Nucleic Acids Res., January 1, 2006; 34(suppl_1): D692 - D696. [Abstract] [Full Text] [PDF]

				C. Sorin, L. Negroni, T. Balliau, H. Corti, M.-P. Jacquemot, M. Davanture, G. Sandberg, M. Zivy, and C. Bellini Proteomic Analysis of Different Mutant Genotypes of Arabidopsis Led to the Identification of 11 Proteins Correlating with Adventitious Root Development Plant Physiology, January 1, 2006; 140(1): 349 - 364. [Abstract] [Full Text] [PDF]

				W. Majeran, Y. Cai, Q. Sun, and K. J. van Wijk Functional Differentiation of Bundle Sheath and Mesophyll Maize Chloroplasts Determined by Comparative Proteomics PLANT CELL, November 1, 2005; 17(11): 3111 - 3140. [Abstract] [Full Text] [PDF]

				M. Hajduch, A. Ganapathy, J. W. Stein, and J. J. Thelen A Systematic Proteomic Study of Seed Filling in Soybean. Establishment of High-Resolution Two-Dimensional Reference Maps, Expression Profiles, and an Interactive Proteome Database Plant Physiology, April 1, 2005; 137(4): 1397 - 1419. [Abstract] [Full Text] [PDF]

				S. Schiltz, N. Munier-Jolain, C. Jeudy, J. Burstin, and C. Salon Dynamics of Exogenous Nitrogen Partitioning and Nitrogen Remobilization from Vegetative Organs in Pea Revealed by 15N in Vivo Labeling throughout Seed Filling Plant Physiology, April 1, 2005; 137(4): 1463 - 1473. [Abstract] [Full Text] [PDF]