First published online August 6, 2004; 10.1104/pp.104.044891
Plant Physiology 135:2318-2329 (2004)
© 2004 American Society of Plant Biologists
ENVIRONMENTAL STRESS AND ADAPTATION
Novel Regulation of Aquaporins during Osmotic Stress1
Rosario Vera-Estrella*,
Bronwyn J. Barkla,
Hans J. Bohnert and
Omar Pantoja
Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de Mexico, Cuernavaca, Morelos, 62250, Mexico (R.V.-E., B.J.B., O.P.); and Departments of Plant Biology and Crop Sciences, University of Illinois, Urbana, Illinois 61801–3838 (H.J.B.)
Aquaporin protein regulation and redistribution in response to osmotic stress was investigated. Ice plant (Mesembryanthemum crystallinum) McTIP1;2 (McMIPF) mediated water flux when expressed in Xenopus leavis oocytes. Mannitol-induced water imbalance resulted in increased protein amounts in tonoplast fractions and a shift in protein distribution to other membrane fractions, suggesting aquaporin relocalization. Indirect immunofluorescence labeling also supports a change in membrane distribution for McTIP1;2 and the appearance of a unique compartment where McTIP1;2 is expressed. Mannitol-induced redistribution of McTIP1;2 was arrested by pretreatment with brefeldin A, wortmannin, and cytochalasin D, inhibitors of vesicle trafficking-related processes. Evidence suggests a role for glycosylation and involvement of a cAMP-dependent signaling pathway in McTIP1;2 redistribution. McTIP1;2 redistribution to endosomal compartments may be part of a homeostatic process to restore and maintain cellular osmolarity under osmotic-stress conditions.
1 This work was supported by the Consejo Nacional de Ciencia y Tecnología (grant nos. 31794N to R.V.-E. and 33054N to O.P.) and by the National Science Foundation International Program (USA-Mexico; to O.P. and H.J.B.).
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.104.044891.
* Corresponding author; e-mail rosario{at}ibt.unam.mx; fax 777–3114691.
Received April 22, 2004;
returned for revision May 31, 2004;
accepted June 4, 2004.
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