Plant Physiology 137:1397-1419 (2005)
© 2005 American Society of Plant Biologists
GENETICS, GENOMICS, AND MOLECULAR EVOLUTION
A Systematic Proteomic Study of Seed Filling in Soybean. Establishment of High-Resolution Two-Dimensional Reference Maps, Expression Profiles, and an Interactive Proteome Database1,[w]
Martin Hajduch,
Ashwin Ganapathy,
Joel W. Stein and
Jay J. Thelen*
Department of Biochemistry, Life Sciences Center (M.H., J.J.T.), and Computer Science Department (A.G., J.W.S.), University of Missouri, Columbia, Missouri 65211
A high-throughput proteomic approach was employed to determine the expression profile and identity of hundreds of proteins during seed filling in soybean (Glycine max) cv Maverick. Soybean seed proteins were analyzed at 2, 3, 4, 5, and 6 weeks after flowering using two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry. This led to the establishment of high-resolution proteome reference maps, expression profiles of 679 spots, and corresponding matrix-assisted laser desorption ionization time-of-flight mass spectrometry spectra for each spot. Database searching with these spectra resulted in the identification of 422 proteins representing 216 nonredundant proteins. These proteins were classified into 14 major functional categories. Proteins involved in metabolism, protein destination and storage, metabolite transport, and disease/defense were the most abundant. For each functional category, a composite expression profile is presented to gain insight into legume seed physiology and the general regulation of proteins associated with each functional class. Using this approach, an overall decrease in metabolism-related proteins versus an increase in proteins associated with destination and storage was observed during seed filling. The accumulation of unknown proteins, sucrose transport and cleavage enzymes, cysteine and methionine biosynthesis enzymes, 14-3-3-like proteins, lipoxygenases, storage proteins, and allergenic proteins during seed filling is also discussed. A user-intuitive database (http://oilseedproteomics.missouri.edu) was developed to access these data for soybean and other oilseeds currently being investigated.
1 This work was supported by the National Science Foundation Plant Genome Research Program (Young Investigator Award no. DBI–0332418).
[w] The online version of this article contains Web-only data.
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.104.056614.
* Corresponding author; e-mail thelenj{at}missouri.edu; fax 573–884–9676.
Received November 16, 2004;
returned for revision December 29, 2004;
accepted December 30, 2004.
Related articles in Plant Physiol.:
- On the Inside
- Peter V. Minorsky
Plant Physiol. 2005 137: 1171-1172.
[Full Text]
This article has been cited by other articles:
|
|
|
|
 
S. B. Xu, T. Li, Z. Y. Deng, K. Chong, Y. Xue, and T. Wang
Dynamic Proteomic Analysis Reveals a Switch between Central Carbon Metabolism and Alcoholic Fermentation in Rice Filling Grains
Plant Physiology,
October 1, 2008;
148(2):
908 - 925.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Schmidt and E. M. Herman
Suppression of Soybean Oleosin Produces Micro-Oil Bodies that Aggregate into Oil Body/ER Complexes
Mol Plant,
September 15, 2008;
(2008)
ssn049v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. K. Agrawal, M. Hajduch, K. Graham, and J. J. Thelen
In-Depth Investigation of the Soybean Seed-Filling Proteome and Comparison with a Parallel Study of Rapeseed
Plant Physiology,
September 1, 2008;
148(1):
504 - 518.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Wang, I. Ladunga, A. R. Miller, K. M. Horken, T. Plucinak, D. P. Weeks, and C. P. Bailey
The Small Ubiquitin-Like Modifier (SUMO) and SUMO-Conjugating System of Chlamydomonas reinhardtii
Genetics,
May 1, 2008;
179(1):
177 - 192.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Gallardo, C. Firnhaber, H. Zuber, D. Hericher, M. Belghazi, C. Henry, H. Kuster, and R. Thompson
A Combined Proteome and Transcriptome Analysis of Developing Medicago truncatula Seeds: Evidence for Metabolic Specialization of Maternal and Filial Tissues
Mol. Cell. Proteomics,
December 1, 2007;
6(12):
2165 - 2179.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. H. Le, J. A. Wagmaister, T. Kawashima, A. Q. Bui, J. J. Harada, and R. B. Goldberg
Using Genomics to Study Legume Seed Development
Plant Physiology,
June 1, 2007;
144(2):
562 - 574.
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Mechin, C. Thevenot, M. Le Guilloux, J.-L. Prioul, and C. Damerval
Developmental Analysis of Maize Endosperm Proteome Suggests a Pivotal Role for Pyruvate Orthophosphate Dikinase
Plant Physiology,
March 1, 2007;
143(3):
1203 - 1219.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Dai, T. Chen, K. Chong, Y. Xue, S. Liu, and T. Wang
Proteomics Identification of Differentially Expressed Proteins Associated with Pollen Germination and Tube Growth Reveals Characteristics of Germinated Oryza sativa Pollen
Mol. Cell. Proteomics,
February 1, 2007;
6(2):
207 - 230.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Dhaubhadel, M. Gijzen, P. Moy, and M. Farhangkhoee
Transcriptome Analysis Reveals a Critical Role of CHS7 and CHS8 Genes for Isoflavonoid Synthesis in Soybean Seeds
Plant Physiology,
January 1, 2007;
143(1):
326 - 338.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. K. Agrawal and J. J. Thelen
Large Scale Identification and Quantitative Profiling of Phosphoproteins Expressed during Seed Filling in Oilseed Rape
Mol. Cell. Proteomics,
November 1, 2006;
5(11):
2044 - 2059.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Fait, R. Angelovici, H. Less, I. Ohad, E. Urbanczyk-Wochniak, A. R. Fernie, and G. Galili
Arabidopsis Seed Development and Germination Is Associated with Temporally Distinct Metabolic Switches
Plant Physiology,
November 1, 2006;
142(3):
839 - 854.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. M. Joseph, T. Hymowitz, M. A. Schmidt, and E. M. Herman
Evaluation of Glycine Germplasm for Nulls of the Immunodominant Allergen P34/Gly m Bd 30k
Crop Sci.,
June 20, 2006;
46(4):
1755 - 1763.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Hajduch, J. E. Casteel, K. E. Hurrelmeyer, Z. Song, G. K. Agrawal, and J. J. Thelen
Proteomic Analysis of Seed Filling in Brassica napus. Developmental Characterization of Metabolic Isozymes Using High-Resolution Two-Dimensional Gel Electrophoresis
Plant Physiology,
May 1, 2006;
141(1):
32 - 46.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Boudet, J. Buitink, F. A. Hoekstra, H. Rogniaux, C. Larre, P. Satour, and O. Leprince
Comparative Analysis of the Heat Stable Proteome of Radicles of Medicago truncatula Seeds during Germination Identifies Late Embryogenesis Abundant Proteins Associated with Desiccation Tolerance
Plant Physiology,
April 1, 2006;
140(4):
1418 - 1436.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Fujiwara, K. Umemura, T. Kawasaki, and K. Shimamoto
Proteomics of Rac GTPase Signaling Reveals Its Predominant Role in Elicitor-Induced Defense Response of Cultured Rice Cells
Plant Physiology,
February 1, 2006;
140(2):
734 - 745.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Majeran, Y. Cai, Q. Sun, and K. J. van Wijk
Functional Differentiation of Bundle Sheath and Mesophyll Maize Chloroplasts Determined by Comparative Proteomics
PLANT CELL,
November 1, 2005;
17(11):
3111 - 3140.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
