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First published online September 23, 2005; 10.1104/pp.105.068924

Plant Physiology 139:779-789 (2005)
© 2005 American Society of Plant Biologists

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DEVELOPMENT AND HORMONE ACTION

Characterization of Terfestatin A, a New Specific Inhibitor for Auxin Signaling1,[w]

Atsushi Yamazoe, Ken-ichiro Hayashi, Stefan Kepinski, Ottoline Leyser and Hiroshi Nozaki*

Department of Biochemistry, Okayama University of Science, Okayama City 700–0005, Japan (A.Y., K.-i.H., H.N.); and Department of Biology, University of York, York YO10 5YW, United Kingdom (S.K., O.L.)

Terfestatin A (TrfA), terphenyl-{beta}-glucoside, was isolated from Streptomyces sp. F40 in a forward screen for compounds that inhibit the expression of auxin-inducible genes in Arabidopsis (Arabidopsis thaliana). TrfA specifically and competitively inhibited the expression of primary auxin-inducible genes in Arabidopsis roots, but did not affect the expression of genes regulated by other plant hormones such as abscisic acid and cytokinin. TrfA also blocked the auxin-enhanced degradation of auxin/indole-3-acetic acid (Aux/IAA) repressor proteins without affecting the auxin-stimulated interaction between Aux/IAAs and the F-box protein TIR1. TrfA treatment antagonized auxin responses in roots, including primary root inhibition, lateral root initiation, root hair promotion, and root gravitropism, but had only limited effects on shoot auxin responses. Taken together, these results indicate that TrfA acts as a modulator of Aux/IAA stability and thus provides a new tool for dissecting auxin signaling.

1 This work was supported by a Grant-in-Aid for Scientific Research (grant no. KAKEN16710162) to K.H. from the Ministry of Education, Culture, Sports, Science, and Technology of Japan, and in part by the Biotechnology and Biological Sciences Research Council (grant no. 87/G14634 to O.L.).

[w] The online version of this article contains Web-only data.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.105.068924.

* Corresponding author; e-mail hayashi{at}dbc.ous.ac.jp; fax 086–256–9556.

Received July 27, 2005; returned for revision August 9, 2005; accepted August 19, 2005.


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