Plant Physiol. Drug Metab Dispos
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online September 29, 2006; 10.1104/pp.106.085209

Plant Physiology 142:1304-1317 (2006)
© 2006 American Society of Plant Biologists

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
142/3/1304    most recent
pp.106.085209v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (15)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Orsel, M.
Right arrow Articles by Miller, A. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Orsel, M.
Right arrow Articles by Miller, A. J.
Agricola
Right arrow Articles by Orsel, M.
Right arrow Articles by Miller, A. J.
ENVIRONMENTAL STRESS AND ADAPTATION TO STRESS

Characterization of a Two-Component High-Affinity Nitrate Uptake System in Arabidopsis. Physiology and Protein-Protein Interaction1,[W]

Mathilde Orsel2, Franck Chopin, Olivier Leleu, Susan J. Smith, Anne Krapp, Françoise Daniel-Vedele and Anthony J. Miller*

Crop Performance and Improvement Division, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, United Kingdom (M.O., S.J.S., A.J.M.); and Unité de la Nutrition Azotée des Plantes, Institut National de la Recherche Agronomique, F–78026 Versailles cedex, France (F.C., O.L., A.K., F.D.-V.)

The identification of a family of NAR2-type genes in higher plants showed that there was a homolog in Arabidopsis (Arabidopsis thaliana), AtNAR2.1. These genes encode part of a two-component nitrate high-affinity transport system (HATS). As the Arabidopsis NRT2 gene family of nitrate transporters has been characterized, we tested the idea that AtNAR2.1 and AtNRT2.1 are partners in a two-component HATS. Results using the yeast split-ubiquitin system and Xenopus oocyte expression showed that the two proteins interacted to give a functional HATS. The growth and nitrogen (N) physiology of two Arabidopsis gene knockout mutants, atnrt2.1-1 and atnar2.1-1, one for each partner protein, were compared. Both types of plants had lost HATS activity at 0.2 mM nitrate, but the effect was more severe in atnar2.1-1 plants. The relationship between plant N status and nitrate transporter expression revealed a pattern that was characteristic of N deficiency that was again stronger in atnar2.1-1. Plants resulting from a cross between both mutants (atnrt2.1-1 x atnar2.1-1) showed a phenotype like that of the atnar2.1-1 mutant when grown in 0.5 mM nitrate. Lateral root assays also revealed growth differences between the two mutants, confirming that atnar2.1-1 had a stronger phenotype. To show that the impaired HATS did not result from the decreased expression of AtNRT2.1, we tested if constitutive root expression of a tobacco (Nicotiana plumbaginifolia) gene, NpNRT2.1, previously been shown to complement atnrt2.1-1, can restore HATS to the atnar2.1-1 mutant. These plants did not recover wild-type nitrate HATS. Taken together, these results show that AtNAR2.1 is essential for HATS of nitrate in Arabidopsis.

1 This work was supported by the European Union (grants no. BIO4CT972231, Research Training Network "Plant use of nitrate" HPRN–CT–2002–00247, and Gabi-Génoplante joint project AF2001/092). Rothamsted Research is grant aided by the Biotechnology and Biological Sciences Research Council of the United Kingdom.

2 Present address: Ameloriation des Plantes et Biotechnolgies Vegetales, UMR 118, INRA-Agrocampus Rennes, BP 35327, 35653 Le Rheu cedex, France.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Anthony J. Miller (tony.miller@bbsrc.ac.uk).

[W] The online version of this article contains Web-only data.

www.plantphysiol.org/cgi/doi/10.1104/pp.106.085209

* Corresponding author; e-mail tony.miller{at}bbsrc.ac.uk; fax 44–1582–763010.

Received June 16, 2006; accepted September 19, 2006; published September 29, 2006.




This article has been cited by other articles:


Home page
 J Exp BotHome page
G. Rahim, S. Bischof, F. Kessler, and B. Agne
In vivo interaction between atToc33 and atToc159 GTP-binding domains demonstrated in a plant split-ubiquitin system
J. Exp. Bot., November 14, 2008; (2008) ern283v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Kant, Y.-M. Bi, E. Weretilnyk, S. Barak, and S. J. Rothstein
The Arabidopsis Halophytic Relative Thellungiella halophila Tolerates Nitrogen-Limiting Conditions by Maintaining Growth, Nitrogen Uptake, and Assimilation
Plant Physiology, July 1, 2008; 147(3): 1168 - 1180.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Sienkiewicz-Porzucek, A. Nunes-Nesi, R. Sulpice, J. Lisec, D. C. Centeno, P. Carillo, A. Leisse, E. Urbanczyk-Wochniak, and A. R. Fernie
Mild Reductions in Mitochondrial Citrate Synthase Activity Result in a Compromised Nitrate Assimilation and Reduced Leaf Pigmentation But Have No Effect on Photosynthetic Performance or Growth
Plant Physiology, May 1, 2008; 147(1): 115 - 127.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
E. Fernandez and A. Galvan
Nitrate Assimilation in Chlamydomonas
Eukaryot. Cell, April 1, 2008; 7(4): 555 - 559.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
L. Lejay, J. Wirth, M. Pervent, J. M.-F. Cross, P. Tillard, and A. Gojon
Oxidative Pentose Phosphate Pathway-Dependent Sugar Sensing as a Mechanism for Regulation of Root Ion Transporters by Photosynthesis
Plant Physiology, April 1, 2008; 146(4): 2036 - 2053.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
C. Richard-Molard, A. Krapp, F. Brun, B. Ney, F. Daniel-Vedele, and S. Chaillou
Plant response to nitrate starvation is determined by N storage capacity matched by nitrate uptake capacity in two Arabidopsis genotypes
J. Exp. Bot., March 1, 2008; 59(4): 779 - 791.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Wirth, F. Chopin, V. Santoni, G. Viennois, P. Tillard, A. Krapp, L. Lejay, F. Daniel-Vedele, and A. Gojon
Regulation of Root Nitrate Uptake at the NRT2.1 Protein Level in Arabidopsis thaliana
J. Biol. Chem., August 10, 2007; 282(32): 23541 - 23552.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. J. Miller, X. Fan, M. Orsel, S. J. Smith, and D. M. Wells
Nitrate transport and signalling
J. Exp. Bot., July 1, 2007; 58(9): 2297 - 2306.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E. Fernandez and A. Galvan
Inorganic nitrogen assimilation in Chlamydomonas
J. Exp. Bot., July 1, 2007; 58(9): 2279 - 2287.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
F. Chopin, M. Orsel, M.-F. Dorbe, F. Chardon, H.-N. Truong, A. J. Miller, A. Krapp, and F. Daniel-Vedele
The Arabidopsis ATNRT2.7 Nitrate Transporter Controls Nitrate Content in Seeds
PLANT CELL, May 1, 2007; 19(5): 1590 - 1602.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
R. Tsujimoto, H. Yamazaki, S.-i. Maeda, and T. Omata
Distinct Roles of Nitrate and Nitrite in Regulation of Expression of the Nitrate Transport Genes in the Moss Physcomitrella patens
Plant Cell Physiol., March 1, 2007; 48(3): 484 - 497.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 2006 by the American Society of Plant Biologists