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First published online March 2, 2007; 10.1104/pp.106.094102 Plant Physiology 144:402-418 (2007) © 2007 American Society of Plant Biologists
Characterization of Lipid Rafts from Medicago truncatula Root Plasma Membranes: A Proteomic Study Reveals the Presence of a Raft-Associated Redox System1,[W]Laboratoire des Interactions Plantes Micro-organismes, Unité Mixte de Recherche, Centre National de la Recherche Scientifique-Institut National de la Recherche Agronomique 2594/441, 31326 Castanet-Tolosan cedex, France (B.L., J.C.); Laboratoire de Biogenèse Membranaire, Unité Mixte de Recherche 5200-Centre National de la Recherche Scientifique-Université Victor Segalen Bordeaux 2, 33076 Bordeaux cedex, France (F.F., F.S.-B., J.-J.B., S.M.); Plate-forme proteomique, IFR40, 31326 Castanet-Tolosan cedex, France (M.R.); Plateau technique Imagerie/Cytologie, Institut National de la Recherche Agronomique, IFR 103, 33883 Villenave d'Ornon cedex, France (J.-P.C.); Institut de Biologie Moléculaire des Plantes, Unité Propre de Recherche-Centre National de la Recherche Scientifique 2357, 67083 Strasbourg cedex, France (M.-A.H.); and Crop Performance and Improvement Division, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, United Kingdom (L.V.M., J.A.N.)
Several studies have provided new insights into the role of sphingolipid/sterol-rich domains so-called lipid rafts of the plasma membrane (PM) from mammalian cells, and more recently from leaves, cell cultures, and seedlings of higher plants. Here we show that lipid raft domains, defined as Triton X-100-insoluble membranes, can also be prepared from Medicago truncatula root PMs. These domains have been extensively characterized by ultrastructural studies as well as by analysis of their content in lipids and proteins. M. truncatula lipid domains are shown to be enriched in sphingolipids and
1 This work was supported by Conseil Régional d'Aquitaine, France (to J.-J.B., S.M., F.S.-B., F.F.), and Agence Nationale de la Recherche (to J.C., S.M.). B.L. and F.F. are funded by fellowships from the Ministère délégué à l'Enseignement supérieur et à la Recherche, France. The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Sébastien Mongrand (sebastien.mongrand{at}biomemb.u-bordeaux2.fr). [W] The online version of this article contains Web-only data. www.plantphysiol.org/cgi/doi/10.1104/pp.106.094102 * Corresponding author; e-mail sebastien.mongrand{at}biomemb.u-bordeaux2.fr; fax 033–5–56–51–83–61. Received December 4, 2006; accepted February 20, 2007; published March 2, 2007. This article has been cited by other articles:
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7-sterols, with spinasterol as the major compound, but also in steryl glycosides and acyl-steryl glycosides. A large number of proteins (i.e. 270) have been identified. Among them, receptor kinases and proteins related to signaling, cellular trafficking, and cell wall functioning were well represented whereas those involved in transport and metabolism were poorly represented. Evidence is also given for the presence of a complete PM redox system in the lipid rafts. 
