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First published online September 28, 2007; 10.1104/pp.107.104422

Plant Physiology 145:747-762 (2007)
© 2007 American Society of Plant Biologists

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DEVELOPMENT AND HORMONE ACTION

Global Expression Profiling Applied to the Analysis of Arabidopsis Stamen Development1,[W],[OA]

Márcio Alves-Ferreira, Frank Wellmer2, Aline Banhara, Vijaya Kumar, José Luis Riechmann and Elliot M. Meyerowitz*

California Institute of Technology, Division of Biology, Pasadena, California 91125 (M.A.-F., F.W., V.K., J.L.R., E.M.M.); and Department of Genetics, Federal University of Rio de Janeiro, Centro de Ciências da Saúde, 21949900 Rio de Janeiro, Brazil (M.A.-F., A.B.)

To obtain detailed information about gene expression during stamen development in Arabidopsis (Arabidopsis thaliana), we compared, by microarray analysis, the gene expression profile of wild-type inflorescences to those of the floral mutants apetala3, sporocyteless/nozzle, and male sterile1 (ms1), in which different aspects of stamen formation are disrupted. These experiments led to the identification of groups of genes with predicted expression at early, intermediate, and late stages of stamen development. Validation experiments using in situ hybridization confirmed the predicted expression patterns. Additional experiments aimed at characterizing gene expression specifically during microspore formation. To this end, we compared the gene expression profiles of wild-type flowers of distinct developmental stages to those of the ms1 mutant. Computational analysis of the datasets derived from this experiment led to the identification of genes that are likely involved in the control of key developmental processes during microsporogenesis. We also identified a large number of genes whose expression is prolonged in ms1 mutant flowers compared to the wild type. This result suggests that MS1, which encodes a putative transcriptional regulator, is involved in the stage-specific repression of these genes. Lastly, we applied reverse genetics to characterize several of the genes identified in the microarray experiments and uncovered novel regulators of microsporogenesis, including the transcription factor MYB99 and a putative phosphatidylinositol 4-kinase.

1 This work was supported by the National Institutes of Health (grant no. GM45697 to E.M.M.), the Millard and Muriel Jacobs Genetics and Genomics Laboratory at the California Institute of Technology, the Conselho Nacional de Desenvolvimento Científico e Tecnológico (grant nos. 307219/2004–6, 400767/2004–0, and 475666/2004–6 to M.A.-F. and fellowship to A.B.), the Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (grant no. E–26/171.332/2004 to M.A.-F. and fellowship to A.B.), the International Foundation for Science (grant no. C/3962–1 to M.A.-F.), the International Basic Sciences Programme (grant no. IBSP/UNESCO–3–BR–28 to M.A.-F.), and Aventis Crop Sciences (fellowship to M.A.-F.).

2 Present address: Smurfit Institute of Genetics, Trinity College Dublin, Dublin 2, Ireland.

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Elliot M. Meyerowitz (meyerow{at}caltech.edu).

[W] The online version of this article contains Web-only data.

[OA] Open Access articles can be viewed online without a subscription.

www.plantphysiol.org/cgi/doi/10.1104/pp.107.104422

* Corresponding author; e-mail meyerow{at}caltech.edu.

Received June 21, 2007; accepted September 14, 2007; published September 28, 2007.

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