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First published online March 19, 2008; 10.1104/pp.107.115246

Plant Physiology 147:30-40 (2008)
© 2008 American Society of Plant Biologists

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BREAKTHROUGH TECHNOLOGIES

Global Characterization of Cell-Specific Gene Expression through Fluorescence-Activated Sorting of Nuclei1,[W],[OA]

Changqing Zhang, Roger A. Barthelson, Georgina M. Lambert and David W. Galbraith*

Department of Plant Sciences (C.Z., R.A.B., G.M.L., D.W.G.), and BIO5 Institute for Collaborative Bioresearch (R.A.B., G.M.L., D.W.G.), The University of Arizona, Tucson, Arizona 85721

We describe a simple and highly effective means for global identification of genes that are expressed within specific cell types within complex tissues. It involves transgenic expression of nuclear-targeted green fluorescent protein in a cell-type-specific manner. The fluorescent nuclei are then purified from homogenates by fluorescence-activated sorting, and the RNAs employed as targets for microarray hybridization. We demonstrate the validity of the approach through the identification of 12 genes that are selectively expressed in phloem.

1 This work was supported by the National Science Foundation Plant Genome Research Program (grant DBI–0211857 to D.W.G.).

The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: David W. Galbraith (galbraith{at}arizona.edu).

[W] The online version of this article contains Web-only data.

[OA] Open Access article can be viewed online without a subscription.

www.plantphysiol.org/cgi/doi/10.1104/pp.107.115246

* Corresponding author; e-mail galbraith{at}arizona.edu.

Received December 19, 2007; accepted March 13, 2008; published March 19, 2008.

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Copyright © 2008 by the American Society of Plant Biologists