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GENOME ANALYSIS

A Genome-Wide Functional Investigation into the Roles of Receptor-Like Proteins in Arabidopsis^1,[W],[OA]

Plant Research International, B.V., Business Unit of Bioscience, 6700 AA Wageningen, The Netherlands (G.W., C.M.L.); Laboratory of Phytopathology, Wageningen University, 6709 PD Wageningen, The Netherlands (U.E., P.J.G.M.W., B.P.H.J.T.); Warwick HRI, University of Warwick, Wellesbourne, Warwick CV35 9EF, United Kingdom (B.K., K.B., A.W.-T., M.T.); Imperial College London, Division of Biology, Wye Campus, Wye, Ashford, Kent TN25 5AH, United Kingdom (J.W.M., A.F., K.M.); Center for Signal Transduction and Metabolomics, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China (C.-M.L.); and Sainsbury Laboratory, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom (C.Z., J.D.G.J.)

Receptor-like proteins (RLPs) are cell surface receptors that typically consist of an extracellular leucine-rich repeat domain, a transmembrane domain, and a short cytoplasmatic tail. In several plant species, RLPs have been found to play a role in disease resistance, such as the tomato (Solanum lycopersicum) Cf and Ve proteins and the apple (Malus domestica) HcrVf2 protein that mediate resistance against the fungal pathogens Cladosporium fulvum, Verticillium spp., and Venturia inaequalis, respectively. In addition, RLPs play a role in plant development; Arabidopsis (Arabidopsis thaliana) TOO MANY MOUTHS (TMM) regulates stomatal distribution, while Arabidopsis CLAVATA2 (CLV2) and its functional maize (Zea mays) ortholog FASCINATED EAR2 regulate meristem maintenance. In total, 57 RLP genes have been identified in the Arabidopsis genome and a genome-wide collection of T-DNA insertion lines was assembled. This collection was functionally analyzed with respect to plant growth and development and sensitivity to various stress responses, including susceptibility toward pathogens. A number of novel developmental phenotypes were revealed for our CLV2 and TMM insertion mutants. In addition, one AtRLP gene was found to mediate abscisic acid sensitivity and another AtRLP gene was found to influence nonhost resistance toward Pseudomonas syringae pv phaseolicola. This genome-wide collection of Arabidopsis RLP gene T-DNA insertion mutants provides a tool for future investigations into the biological roles of RLPs.

² These authors contributed equally to the article.

The authors responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) are: Bart P.H.J. Thomma (bart.thomma{at}wur.nl) and Mahmut Tör (mahmut.tor{at}warwick.ac.uk).

^[W] The online version of this article contains Web-only data.

^[OA] Open Access articles can be viewed online without a subscription.

www.plantphysiol.org/cgi/doi/10.1104/pp.108.119487

^* Corresponding author; e-mail bart.thomma{at}wur.nl.

Received March 20, 2008; accepted April 11, 2008; published April 23, 2008.