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Research ArticleMembranes and Bioenergetics
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Tonoplast-Bound Protein Kinase Phosphorylates Tonoplast Intrinsic Protein

Kenneth D. Johnson, Maarten J. Chrispeels
Kenneth D. Johnson
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Maarten J. Chrispeels
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Published December 1992. DOI: https://doi.org/10.1104/pp.100.4.1787

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Abstract

Tonoplast intrinsic protein (TIP) is a member of a family of putative membrane channels found in bacteria, animals, and plants. Plants have seed-specific, vegetative/reproductive organ-specific, and water-stress-induced forms of TIP. Here, we report that the seed-specific TIP is a phosphoprotein whose phosphorylation can be monitored in vivo by allowing bean cotyledons to take up [32P]orthophosphate and in vitro by incubating purified tonoplasts with γ-labeled [32P]ATP. Characterization of the in vitro phosphorylation of TIP indicates that a membrane-bound protein kinase phosphorylates TIP in a Ca2+-dependent manner. The capacity of the isolated tonoplast membranes to phosphorylate TIP declined markedly during seed germination, and this decline occurred well before the development-mediated decrease in TIP occurs. Phosphoamino acid analysis of purified, radiolabeled TIP showed that serine is the major, if not only, phosphorylated residue, and cyanogen bromide cleavage yielded a single radioactive peptide peak on a reverse-phase high-performance liquid chromatogram. Estimation of the molecular mass of the cyanogen bromide phosphopeptide by laser desorption mass spectroscopy led to its identification as the hydrophilic N-terminal domain of TIP. The putative phosphate-accepting serine residue occurs in a consensus phosphorylation site for serine/threonine protein kinases.

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Tonoplast-Bound Protein Kinase Phosphorylates Tonoplast Intrinsic Protein
Kenneth D. Johnson, Maarten J. Chrispeels
Plant Physiology Dec 1992, 100 (4) 1787-1795; DOI: 10.1104/pp.100.4.1787

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Tonoplast-Bound Protein Kinase Phosphorylates Tonoplast Intrinsic Protein
Kenneth D. Johnson, Maarten J. Chrispeels
Plant Physiology Dec 1992, 100 (4) 1787-1795; DOI: 10.1104/pp.100.4.1787
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Plant Physiology
Vol. 100, Issue 4
December 1992
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More in this TOC Section

  • Effects of Deuterium Oxide on Growth, Proton Extrusion, Potassium Influx, and in Vitro Plasma Membrane Activities in Maize Root Segments
  • Electrically Induced Protoplast Fusion Using Pulse Electric Fields for Dielectrophoresis
  • Isolation and Characterization of the Tricarboxylate Transporter from Pea Mitochondria
Show more Membranes and Bioenergetics

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