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OtherMOLECULAR BIOLOGY AND GENE REGULATION
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Molecular Basis of the Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Activase Mutation in Arabidopsis thaliana Is a Guanine-to-Adenine Transition at the 5[prime]-Splice Junction of Intron 3

B. M. Orozco, C. R. McClung, J. M. Werneke, W. L. Ogren
B. M. Orozco
Department of Plant Biology, University of Illinois at Urbana-Champaign (B.M.O., J.M.W., W.L.O); Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire (C.R.M.); and Photosynthesis Research Unit, Agricultural Research Service, United States Department of Agriculture, Urbana, Illinois 61801–3838 (W.L.O.)
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C. R. McClung
Department of Plant Biology, University of Illinois at Urbana-Champaign (B.M.O., J.M.W., W.L.O); Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire (C.R.M.); and Photosynthesis Research Unit, Agricultural Research Service, United States Department of Agriculture, Urbana, Illinois 61801–3838 (W.L.O.)
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J. M. Werneke
Department of Plant Biology, University of Illinois at Urbana-Champaign (B.M.O., J.M.W., W.L.O); Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire (C.R.M.); and Photosynthesis Research Unit, Agricultural Research Service, United States Department of Agriculture, Urbana, Illinois 61801–3838 (W.L.O.)
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W. L. Ogren
Department of Plant Biology, University of Illinois at Urbana-Champaign (B.M.O., J.M.W., W.L.O); Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire (C.R.M.); and Photosynthesis Research Unit, Agricultural Research Service, United States Department of Agriculture, Urbana, Illinois 61801–3838 (W.L.O.)
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Published May 1993. DOI: https://doi.org/10.1104/pp.102.1.227

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Abstract

Analysis of the ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activase gene and gene products from Arabidopsis thaliana wild-type plants and the Rubisco activase-deficient mutant strain showed that the rca mutation caused GT to be changed to AT at the 5[prime]-splice junction of intron 3 in the six-intron pre-mRNA. Northern blot analysis, genomic and cDNA sequencing, and primer extension analysis indicated that the mutation causes inefficient and incomplete splicing of the pre-mRNA, resulting in the accumulation of three aberrant mRNAs. One mutant mRNA was identical with wild-type mRNA except that it included intron 3, a second mRNA comprised intron 3 and exons 4 through 7, and the third mRNA contained exons 1 through 3. The G-to-A transition is consistent with the known mechanism of mutagenesis by ethyl methanesulfonate, the mutagen used to create the Rubisco activase-deficient strain.

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Molecular Basis of the Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Activase Mutation in Arabidopsis thaliana Is a Guanine-to-Adenine Transition at the 5[prime]-Splice Junction of Intron 3
B. M. Orozco, C. R. McClung, J. M. Werneke, W. L. Ogren
Plant Physiology May 1993, 102 (1) 227-232; DOI: 10.1104/pp.102.1.227

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Molecular Basis of the Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Activase Mutation in Arabidopsis thaliana Is a Guanine-to-Adenine Transition at the 5[prime]-Splice Junction of Intron 3
B. M. Orozco, C. R. McClung, J. M. Werneke, W. L. Ogren
Plant Physiology May 1993, 102 (1) 227-232; DOI: 10.1104/pp.102.1.227
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Plant Physiology
Vol. 102, Issue 1
May 1993
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More in this TOC Section

  • Genes Galore: A Summary of Methods for Accessing Results from Large-Scale Partial Sequencing of Anonymous Arabidopsis cDNA Clones
  • Expression of the Arabidopsis Gene Akr Coincides with Chloroplast Development
  • Molecular Genetics of the Maize (Zea mays L.) Aspartate Kinase-Homoserine Dehydrogenase Gene Family
Show more Molecular Biology and Gene Regulation

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