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OtherCELL BIOLOGY AND SIGNAL TRANSDUCTION
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Blue Light Induces Phosphorylation at Seryl Residues on a Pea (Pisum sativum L.) Plasma Membrane Protein

T. W. Short, M. Porst, J. Palmer, E. Fernbach, W. R. Briggs
T. W. Short
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M. Porst
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J. Palmer
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E. Fernbach
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W. R. Briggs
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Published April 1994. DOI: https://doi.org/10.1104/pp.104.4.1317

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Abstract

We have partially characterized the blue-light-stimulated in vitro phosphorylation of a membrane protein from etiolated Pisum sativum L. stems. Properties of the response have implicated its involvement in signal transduction of phototropic stimuli (T.W. Short, W.R. Briggs [1990] Plant Physiol 92: 179–185; P. Reymond, T.W. Short, W.R. Briggs [1992] Proc Natl Acad Sci USA 89: 4718–4721). Analysis of proteolysis products and phosphoamino acidanalysis indicate that the substrate protein is phosphorylated on multiple seryl residues. Kinetics of the in vitro reaction show phosphorylation to be complete within 2 to 5 min at 30[deg]C in either light-exposed or dark-control plasma membrane preparations, regardless of whether the membranes were first solubilized in Triton X-100. Nucleotide competition assays show the kinase to be ATP specific. The pH optimum covers a broad range with a maximum near 7.5. A wide array of salts inhibits the phosphorylation at high concentrations, but millimolar concentrations of Mg2+ are required to form Mg.ATP complexes for maximal activity, whereas excess free Mg2+ or Ca2+ are not required for the reaction.

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Blue Light Induces Phosphorylation at Seryl Residues on a Pea (Pisum sativum L.) Plasma Membrane Protein
T. W. Short, M. Porst, J. Palmer, E. Fernbach, W. R. Briggs
Plant Physiology Apr 1994, 104 (4) 1317-1324; DOI: 10.1104/pp.104.4.1317

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Blue Light Induces Phosphorylation at Seryl Residues on a Pea (Pisum sativum L.) Plasma Membrane Protein
T. W. Short, M. Porst, J. Palmer, E. Fernbach, W. R. Briggs
Plant Physiology Apr 1994, 104 (4) 1317-1324; DOI: 10.1104/pp.104.4.1317
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Plant Physiology
Vol. 104, Issue 4
Apr 1994
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More in this TOC Section

  • The Cell Wall of the Arabidopsis Pollen Tube—Spatial Distribution, Recycling, and Network Formation of Polysaccharides
  • Systems Dynamic Modeling of a Guard Cell Cl− Channel Mutant Uncovers an Emergent Homeostatic Network Regulating Stomatal Transpiration
  • Architecture-Based Multiscale Computational Modeling of Plant Cell Wall Mechanics to Examine the Hydrogen-Bonding Hypothesis of the Cell Wall Network Structure Model
Show more CELL BIOLOGY AND SIGNAL TRANSDUCTION

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