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OtherMOLECULAR BIOLOGY AND GENE REGULATION
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Plant [delta]-Aminolevulinic Acid Dehydratase (Expression in Soybean Root Nodules and Evidence for a Bacterial Lineage of the Alad Gene)

C. M. Kaczor, M. W. Smith, I. Sangwan, M. R. O'Brian
C. M. Kaczor
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M. W. Smith
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I. Sangwan
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M. R. O'Brian
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Published April 1994. DOI: https://doi.org/10.1104/pp.104.4.1411

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Abstract

We isolated a soybean (Glycine max) cDNA encoding the heme and chlorophyll synthesis enzyme [delta]-aminolevulinic acid (ALA) dehydratase by functional complementation of an Escherichia coli hemB mutant, and we designated the gene Alad. ALA dehydratase was strongly expressed in nodules but not in uninfected roots, although Alad mRNA was only 2- to 3-fold greater in the symbiotic tissue. Light was not essential for expression of Alad in leaves of dark-grown etiolated plantlets as discerned by mRNA, protein, and enzyme activity levels; hence, its expression in subterranean nodules was not unique in that regard. The data show that soybean can metabolize the ALA it synthesizes in nodules, which argues in favor of tetrapyrrole formation by the plant host in that organ. Molecular phylogenetic analysis of ALA dehydratases from 11 organisms indicated that plant and bacterial enzymes have a common lineage not shared by animals and yeast. We suggest that plant ALA dehydratase is descended from the bacterial endosymbiont ancestor of chloroplasts and that the Alad gene was transferred to the nucleus during plant evolution.

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Plant [delta]-Aminolevulinic Acid Dehydratase (Expression in Soybean Root Nodules and Evidence for a Bacterial Lineage of the Alad Gene)
C. M. Kaczor, M. W. Smith, I. Sangwan, M. R. O'Brian
Plant Physiology Apr 1994, 104 (4) 1411-1417; DOI: 10.1104/pp.104.4.1411

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Plant [delta]-Aminolevulinic Acid Dehydratase (Expression in Soybean Root Nodules and Evidence for a Bacterial Lineage of the Alad Gene)
C. M. Kaczor, M. W. Smith, I. Sangwan, M. R. O'Brian
Plant Physiology Apr 1994, 104 (4) 1411-1417; DOI: 10.1104/pp.104.4.1411
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Plant Physiology
Vol. 104, Issue 4
Apr 1994
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More in this TOC Section

  • Genes Galore: A Summary of Methods for Accessing Results from Large-Scale Partial Sequencing of Anonymous Arabidopsis cDNA Clones
  • Expression of the Arabidopsis Gene Akr Coincides with Chloroplast Development
  • Molecular Genetics of the Maize (Zea mays L.) Aspartate Kinase-Homoserine Dehydrogenase Gene Family
Show more Molecular Biology and Gene Regulation

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