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Localization of Membrane Proteins in the Cyanobacterium Synechococcus sp. PCC7942 (Radial Asymmetry in the Photosynthetic Complexes)

D. M. Sherman, T. A. Troyan, L. A. Sherman
D. M. Sherman
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T. A. Troyan
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L. A. Sherman
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Published September 1994. DOI: https://doi.org/10.1104/pp.106.1.251

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Abstract

Localization of membrane proteins in the cyanobacterium Synechococcus sp. PCC7942 was determined by transmission electron microscopy utilizing immunocytochemistry with cells prepared by freeze-substitution. This preparation procedure maintained cellular morphology and permitted detection of cellular antigens with high sensitivity and low background. Synechococcus sp. PCC7942 is a unicellular cyanobacterium with thylakoids organized in concentric layers toward the periphery of the cell. Cytochrome oxidase was localized almost entirely in the cytoplasmic membrane, whereas a carotenoprotein (P35) was shown to be a cell wall component. The major photosystem II (PSII) proteins (D1, D2 CP43, and CP47) were localized throughout the thylakoids. Proteins of the Cyt b6/f complex were found to have a similar distribution. Thylakoid luminal proteins, such as the Mn-stabilizing protein, were located primarily in the thylakoid, but a small, reproducible fraction was found in the outer compartment. The photosystem I (PSI) reaction center proteins and the ATP synthase proteins were found associated mostly with the outermost thylakoid and with the cytoplasmic membrane. These results indicated that the photosynthetic apparatus is not evenly distributed throughout the thylakoids. Rather, there is a radial asymmetry such that much of the PSI and the ATPase synthase is located in the outermost thylakoid. The relationship of this structure to the photosynthetic mechanism is discussed. It is suggested that the photosystems are separated because of kinetic differences between PSII and PSI, as hypothesized by H.-W. Trissl and C. Wilhelm (Trends Biochem Sci [1993] 18:415–419).

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Localization of Membrane Proteins in the Cyanobacterium Synechococcus sp. PCC7942 (Radial Asymmetry in the Photosynthetic Complexes)
D. M. Sherman, T. A. Troyan, L. A. Sherman
Plant Physiology Sep 1994, 106 (1) 251-262; DOI: 10.1104/pp.106.1.251

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Localization of Membrane Proteins in the Cyanobacterium Synechococcus sp. PCC7942 (Radial Asymmetry in the Photosynthetic Complexes)
D. M. Sherman, T. A. Troyan, L. A. Sherman
Plant Physiology Sep 1994, 106 (1) 251-262; DOI: 10.1104/pp.106.1.251
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Plant Physiology
Vol. 106, Issue 1
Sep 1994
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More in this TOC Section

  • A Novel Gene, pmgA, Specifically Regulates Photosystem Stoichiometry in the CyanobacteriumSynechocystis Species PCC 6803 in Response to High Light
  • Analysis of Respiratory Chain Regulation in Roots of Soybean Seedlings
  • Characterization of a Red Beet Protein Homologous to the Essential 36-Kilodalton Subunit of the Yeast V-Type ATPase
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