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OtherMETABOLISM AND ENZYMOLOGY
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Identification of Posttranslationally Modified 18-Kilodalton Protein from Rice as Eukaryotic Translation Initiation Factor 5A

A. M. Mehta, R. A. Saftner, R. A. Mehta, P. J. Davies
A. M. Mehta
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R. A. Saftner
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R. A. Mehta
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P. J. Davies
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Published December 1994. DOI: https://doi.org/10.1104/pp.106.4.1413

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Abstract

Using anther-derived rice (Oryza sativa L.) cell-suspension cultures, we have identified an 18-kD protein that is posttranslationally modified by spermidine and is influenced by endogenous polyamine levels. The posttranslationally modified residue has been identified as the unusual amino acid hypusine [N[epsilon]-(4-amino-2-hydroxybutyl)lysine] by reverse-phase high-performance liquid chromatography and gas chromatography-mass-spectrometry analyses. Differential labeling of the protein with labeled amines provided evidence that the butylamine moiety of spermidine is the immediate precursor of the hypusine residue in the protein. The eukaryotic translation initiation factor 5A (eIF-5A) is the only known mammalian protein that undergoes a similar posttranslational modification with hypusine. The purified 18-kD protein co-electrophoreses with human translational initiation factor eIF-5A in both isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gels. The purified protein from rice stimulated methionyl-puromycin synthesis in vitro, indicating its functional similarity to mammalian eIF-5A. The results presented provide evidence that the posttranslationally modified 18-kD protein from rice containing hypusine is eIF-5A and suggest the conservation of hypusine-containing translation initiation factor eIF-5A in eukaryotes.

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Identification of Posttranslationally Modified 18-Kilodalton Protein from Rice as Eukaryotic Translation Initiation Factor 5A
A. M. Mehta, R. A. Saftner, R. A. Mehta, P. J. Davies
Plant Physiology Dec 1994, 106 (4) 1413-1419; DOI: 10.1104/pp.106.4.1413

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Identification of Posttranslationally Modified 18-Kilodalton Protein from Rice as Eukaryotic Translation Initiation Factor 5A
A. M. Mehta, R. A. Saftner, R. A. Mehta, P. J. Davies
Plant Physiology Dec 1994, 106 (4) 1413-1419; DOI: 10.1104/pp.106.4.1413
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Plant Physiology
Vol. 106, Issue 4
Dec 1994
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More in this TOC Section

  • Immediate Activation of Respiration in Petroselinum crispum L. in Response to the Phytophthora megasperma f. sp. Glycinea Elicitor
  • The Metabolism of Gibberellin A20 to Gibberellin A1 by Tall and Dwarf Mutants of Oryza sativa and Arabidopsis thaliana
  • A Mutation at the fad8 Locus of Arabidopsis Identifies a Second Chloroplast [omega]-3 Desaturase
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