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OtherGENE REGULATION AND MOLECULAR GENETICS
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A Putative Mg Chelatase Subunit from Arabidopsis thaliana cv C24 (Sequence and Transcript Analysis of the Gene, Import of the Protein into Chloroplasts, and in Situ Localization of the Transcript and Protein

LCD. Gibson, J. L. Marrison, R. M. Leech, P. E. Jensen, D. C. Bassham, M. Gibson, C. N. Hunter
LCD. Gibson
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J. L. Marrison
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R. M. Leech
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P. E. Jensen
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D. C. Bassham
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M. Gibson
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C. N. Hunter
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Published May 1996. DOI: https://doi.org/10.1104/pp.111.1.61

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Abstract

We have isolated and sequenced a cDNA from Arabidopsis thaliana cv C24 that encodes a putative Mg chelatase subunit. The deduced amino acid sequence shows a very high level of identity to a gene previously characterized from Antirrhinum majus (olive) and also high similarity to bchH, a bacterial gene involved in the Mg chelatase reaction of bacteriochlorophyll biosynthesis. We suggest that this gene be called CHL H. Northern blot analyses were used to investigate the expression of CHL H, another putative Mg chelatase gene, ch-42, and ferrochelatase. The CHL H transcript was observed to undergo a dramatic diurnal variation, rising almost to its maximum level by the end of the dark period, then increasing slightly at the onset of the light and declining steadily to a minimum by the end of the light period; in contrast, transcripts for ch-42 and ferrochelatase remained constant. A model is proposed in which the CHL H protein plays a role in regulating the levels of chlorophyll during this cycle. In situ hybridization revealed that the transcripts are located over the surface of the chloroplasts, a feature in common with transcripts for the ch-42 gene. The CHL H protein was imported into the stromal compartment of the chloroplast and processed in an in vitro assay. Immunoblotting showed that the distribution of CHL H protein between the stroma and chloroplast membranes varies depending on the concentration of Mg2+. In situ immunofluorescence was used to establish that the CHL H and CH-42 proteins are localized within the chloroplast in vivo.

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A Putative Mg Chelatase Subunit from Arabidopsis thaliana cv C24 (Sequence and Transcript Analysis of the Gene, Import of the Protein into Chloroplasts, and in Situ Localization of the Transcript and Protein
LCD. Gibson, J. L. Marrison, R. M. Leech, P. E. Jensen, D. C. Bassham, M. Gibson, C. N. Hunter
Plant Physiology May 1996, 111 (1) 61-71; DOI: 10.1104/pp.111.1.61

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A Putative Mg Chelatase Subunit from Arabidopsis thaliana cv C24 (Sequence and Transcript Analysis of the Gene, Import of the Protein into Chloroplasts, and in Situ Localization of the Transcript and Protein
LCD. Gibson, J. L. Marrison, R. M. Leech, P. E. Jensen, D. C. Bassham, M. Gibson, C. N. Hunter
Plant Physiology May 1996, 111 (1) 61-71; DOI: 10.1104/pp.111.1.61
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Plant Physiology
Vol. 111, Issue 1
May 1996
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More in this TOC Section

  • Cloning of Sucrose:Sucrose 1-Fructosyltransferase from Onion and Synthesis of Structurally Defined Fructan Molecules from Sucrose
  • RNA Polymerase Subunits Encoded by the Plastid rpoGenes Are Not Shared with the Nucleus-Encoded Plastid Enzyme
  • Decreased GA1 Content Caused by the Overexpression ofOSH1 Is Accompanied by Suppression of GA 20-Oxidase Gene Expression
Show more GENE REGULATION AND MOLECULAR GENETICS

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