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OtherGENE REGULATION AND MOLECULAR GENETICS
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Differential Expression of the Multigene Family Encoding the Soybean Mitochondrial Alternative Oxidase

P. M. Finnegan, J. Whelan, A. H. Millar, Q. Zhang, M. K. Smith, J. T. Wiskich, D. A. Day
P. M. Finnegan
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J. Whelan
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A. H. Millar
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Q. Zhang
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M. K. Smith
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J. T. Wiskich
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D. A. Day
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Published June 1997. DOI: https://doi.org/10.1104/pp.114.2.455

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Abstract

The alternative oxidase (AOX) of the soybean (Glycine max L.) inner mitochondrial membrane is encoded by a multigene family (Aox) with three known members. Here, the Aox2 and Aox3 primary translation products, deduced from cDNA analysis, were found to be 38.1 and 36.4 kD, respectively. Direct N-terminal sequencing of partially purified AOX from cotyledons demonstrates that the mature proteins are 31.8 and 31.6 kD, respectively, implying that processing occurs upon import of these proteins into the mitochondrion. Sequence comparisons show that the processing of plant AOX proteins occurs at a characteristic site and that the AOX2 and AOX3 proteins are more similar to one another than to other AOX proteins, including soybean AOX1. Transcript analysis using a polymerase chain reaction-based assay in conjunction with immunoblot experiments indicates that soybean Aox genes are differentially expressed in a tissue-dependent manner. Moreover, the relative abundance of both Aox2 transcripts and protein in cotyledons increase upon greening of dark-grown seedlings. These results comprehensively explain the multiple AOX-banding patterns observed on immunoblots of mitochondrial proteins isolated from various soybean tissues by matching protein bands with gene products.

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Differential Expression of the Multigene Family Encoding the Soybean Mitochondrial Alternative Oxidase
P. M. Finnegan, J. Whelan, A. H. Millar, Q. Zhang, M. K. Smith, J. T. Wiskich, D. A. Day
Plant Physiology Jun 1997, 114 (2) 455-466; DOI: 10.1104/pp.114.2.455

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Differential Expression of the Multigene Family Encoding the Soybean Mitochondrial Alternative Oxidase
P. M. Finnegan, J. Whelan, A. H. Millar, Q. Zhang, M. K. Smith, J. T. Wiskich, D. A. Day
Plant Physiology Jun 1997, 114 (2) 455-466; DOI: 10.1104/pp.114.2.455
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Plant Physiology
Vol. 114, Issue 2
Jun 1997
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More in this TOC Section

  • Cloning of Sucrose:Sucrose 1-Fructosyltransferase from Onion and Synthesis of Structurally Defined Fructan Molecules from Sucrose
  • RNA Polymerase Subunits Encoded by the Plastid rpoGenes Are Not Shared with the Nucleus-Encoded Plastid Enzyme
  • Decreased GA1 Content Caused by the Overexpression ofOSH1 Is Accompanied by Suppression of GA 20-Oxidase Gene Expression
Show more GENE REGULATION AND MOLECULAR GENETICS

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