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Soybean Root Nodule Acid Phosphatase

A. R. Penheiter, SMG. Duff, G. Sarath
A. R. Penheiter
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SMG. Duff
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G. Sarath
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Published June 1997. DOI: https://doi.org/10.1104/pp.114.2.597

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Abstract

Acid phosphatases are ubiquitous enzymes that exhibit activity against a variety of substrates in vitro, although little is known about their intracellular function. In this study we report the isolation, characterization, and partial sequence of the major acid phosphatase from soybean (Glycine max L.) root nodules. The phosphatase was purified predominantly as a heterodimer with subunits of 28 and 31 kD; homodimers of both subunits were also observed and exhibited phosphatase activity. In addition to the general phosphatase substrate, p-nitrophenyl phosphate, the heterodimeric form of the enzyme readily hydrolyzed 5[prime]-nucleotides, flavin mononucleotide, and O-phospho-L-Tyr. Low or negligible activity was observed with ATP or polyphosphate. Purified nodule acid phosphatase was stimulated by magnesium, inhibited by calcium and EDTA, and competitively inhibited by cGMP and cAMP with apparent Ki values of 7 and 12 [mu]M, respectively. Partial N-terminal and internal sequencing of the nodule acid phosphatase revealed homology to the soybean vegetative storage proteins. There was a 17-fold increase in enzyme activity and a noticeable increase in protein levels detected by immunoblotting methods during nodule development. Both of these parameters were low in young nodules and reached a peak in mature, functional nodules, suggesting that this enzyme is important for efficient nodule metabolism.

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Soybean Root Nodule Acid Phosphatase
A. R. Penheiter, SMG. Duff, G. Sarath
Plant Physiology Jun 1997, 114 (2) 597-604; DOI: 10.1104/pp.114.2.597

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Soybean Root Nodule Acid Phosphatase
A. R. Penheiter, SMG. Duff, G. Sarath
Plant Physiology Jun 1997, 114 (2) 597-604; DOI: 10.1104/pp.114.2.597
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Plant Physiology
Vol. 114, Issue 2
Jun 1997
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  • Purification, Characterization, and Molecular Cloning of the Gene of a Seed-Specific Antimicrobial Protein from Pokeweed
  • Salicylic Acid Mediated by the Oxidative Burst Is a Key Molecule in Local and Systemic Responses of Cotton Challenged by an Avirulent Race of Xanthomonas campestris pvmalvacearum
  • Expression and Localization of Nitrilase during Symptom Development of the Clubroot Disease in Arabidopsis
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