Skip to main content

Main menu

  • For Authors
    • Submit a Manuscript
    • Instructions for Authors
  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
    • Focus Collections
    • Classics Collection
    • Upcoming Focus Issues
  • Advertisers
  • About
    • About the Journal
    • Editorial Board and Staff
  • Subscribers
  • Librarians
  • More
    • Alerts
    • Contact Us
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Plant Cell Teaching Tools
    • ASPB
    • Plantae

User menu

  • My alerts
  • Log in

Search

  • Advanced search
Plant Physiology
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Plant Cell Teaching Tools
    • ASPB
    • Plantae
  • My alerts
  • Log in
Plant Physiology

Advanced Search

  • For Authors
    • Submit a Manuscript
    • Instructions for Authors
  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
    • Focus Collections
    • Classics Collection
    • Upcoming Focus Issues
  • Advertisers
  • About
    • About the Journal
    • Editorial Board and Staff
  • Subscribers
  • Librarians
  • More
    • Alerts
    • Contact Us
  • Follow plantphysiol on Twitter
  • Visit plantphysiol on Facebook
  • Visit Plantae
OtherPLANT-MICROBE AND PLANT-INSECT INTERACTIONS
You have accessRestricted Access

Construction of a Lotus japonicus Late Nodulin Expressed Sequence Tag Library and Identification of Novel Nodule-Specific Genes

K. Szczyglowski, D. Hamburger, P. Kapranov, F. J. de Bruijn
K. Szczyglowski
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
D. Hamburger
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
P. Kapranov
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
F. J. de Bruijn
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site

Published August 1997. DOI: https://doi.org/10.1104/pp.114.4.1335

  • Article
  • Info & Metrics
  • PDF
Loading
  • Copyright © 1997 by American Society of Plant Biologists

Abstract

A range of novel expressed sequence tags (ESTs) associated with late developmental events during nodule organogenesis in the legume Lotus japonicus were identified using mRNA differential display; 110 differentially displayed polymerase chain reaction products were cloned and analyzed. Of 88 unique cDNAs obtained, 22 shared significant homology to DNA/protein sequences in the respective databases. This group comprises, among others, a nodule-specific homolog of protein phosphatase 2C, a peptide transporter protein, and a nodule-specific form of cytochrome P450. RNA gel-blot analysis of 16 differentially displayed ESTs confirmed their nodule-specific expression pattern. The kinetics of mRNA accumulation of the majority of the ESTs analyzed were found to resemble the expression pattern observed for the L. japonicus leghemoglobin gene. These results indicate that the newly isolated molecular markers correspond to genes induced during late developmental stages of L. japonicus nodule organogenesis and provide important, novel tools for the study of nodulation.

PreviousNext
Back to top

Table of Contents

Download PDF
Email Article

Thank you for your interest in spreading the word on Plant Physiology.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Construction of a Lotus japonicus Late Nodulin Expressed Sequence Tag Library and Identification of Novel Nodule-Specific Genes
(Your Name) has sent you a message from Plant Physiology
(Your Name) thought you would like to see the Plant Physiology web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Construction of a Lotus japonicus Late Nodulin Expressed Sequence Tag Library and Identification of Novel Nodule-Specific Genes
K. Szczyglowski, D. Hamburger, P. Kapranov, F. J. de Bruijn
Plant Physiology Aug 1997, 114 (4) 1335-1346; DOI: 10.1104/pp.114.4.1335

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Request Permissions
Share
Construction of a Lotus japonicus Late Nodulin Expressed Sequence Tag Library and Identification of Novel Nodule-Specific Genes
K. Szczyglowski, D. Hamburger, P. Kapranov, F. J. de Bruijn
Plant Physiology Aug 1997, 114 (4) 1335-1346; DOI: 10.1104/pp.114.4.1335
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • PDF

In this issue

Plant Physiology
Vol. 114, Issue 4
Aug 1997
  • Table of Contents
  • Index by author

More in this TOC Section

  • Purification, Characterization, and Molecular Cloning of the Gene of a Seed-Specific Antimicrobial Protein from Pokeweed
  • Salicylic Acid Mediated by the Oxidative Burst Is a Key Molecule in Local and Systemic Responses of Cotton Challenged by an Avirulent Race of Xanthomonas campestris pvmalvacearum
  • Expression and Localization of Nitrilase during Symptom Development of the Clubroot Disease in Arabidopsis
Show more PLANT-MICROBE AND PLANT-INSECT INTERACTIONS

Similar Articles

Our Content

  • Home
  • Current Issue
  • Plant Physiology Preview
  • Archive
  • Focus Collections
  • Classic Collections
  • The Plant Cell
  • Plant Direct
  • Plantae
  • ASPB

For Authors

  • Instructions
  • Submit a Manuscript
  • Editorial Board and Staff
  • Policies
  • Recognizing our Authors

For Reviewers

  • Instructions
  • Journal Miles
  • Policies

Other Services

  • Permissions
  • Librarian resources
  • Advertise in our journals
  • Alerts
  • RSS Feeds

Copyright © 2021 by The American Society of Plant Biologists

Powered by HighWire