Article Figures & Data
Figures
- Fig. 1.
The effect of light on NPA-induced inhibition of elongation of hypocotyls (A) and roots (B). Growth is normalized to that of seedlings grown without NPA. The fluence rate of the white light was 50 μmol m−2 s−1. Values are the means ± sd from three independent replicates, with at least 10 seedlings per replicate. conc., Concentration.
- Fig. 2.
The effect of fluence rate on inhibition of elongation by 1.0 μm NPA. A, Comparison of hypocotyl lengths in 1.0 μm NPA-treated and untreated seedlings. B, Comparison of root lengths in 1.0 μm NPA-treated and untreated seedlings. C, Percentage inhibition of hypocotyl elongation calculated as growth on 1.0 μm NPA compared with untreated seedlings at the corresponding fluence rate. D, Percentage inhibition of root elongation calculated as growth on 1.0 μm NPA compared with untreated seedlings (see C). Values are the means ± sd from three independent replicates, with at least 10 seedlings per replicate.
- Fig. 3.
The effect of 1.0 μm NPA on the gravity response of hypocotyls (A) and roots (B) of light- and dark-grown seedlings. Data are presented as the sd of the orientation angles of the hypocotyls and roots as a measure of the randomness of orientation.
- Fig. 4.
The effect of light quality on the inhibition of hypocotyl elongation by 1.0 μm NPA. Seedlings were grown under continuous white light (80 μmol m−2s−1), blue light (30 μmol m−2s−1), far-red light (10 μmol m−2s−1), or red light (25 μmol m−2s−1) for 7 d. Values are the means ± sd from three independent replicates, with at least 10 seedlings per replicate.
- Fig. 5.
The effect of blue and far-red light fluence rate on inhibition of hypocotyl elongation by 1.0 μm NPA. The percentage inhibition of elongation is calculated as growth on 1.0 μm NPA compared with untreated seedlings at the corresponding fluence rate. Values are the means ± sd from three independent replicates, with at least 10 seedlings per replicate.
- Fig. 6.
The effect of 1.0 μm NPA on the hypocotyl elongation of photoreceptor mutants and wild types (Ler and Rld). Values are the means ± sd from three independent replicates, with at least 10 seedlings per replicate. The percentage-inhibition values were calculated by determining the percentage inhibition for each NPA-treated seedling using the average length of the appropriate control seedlings. The values for each seedling were then averaged to generate the data points ± sd. Seedlings were grown as described in Figure 4.
- Fig. 7.
Inhibition of lateral root formation by 0.5 μm NPA at various fluence rates. Values are the means ± sd from three independent replicates, with at least 10 seedlings per replicate.
Tables
- Table I.
Effect of 1.0 μm NPA on hypocotyl length ± sd of various hormone mutants grown for 7 d in darkness or white light (80 μmol m−2s−1)
Genotype Light Dark −NPA +NPA Inhibition −NPA +NPA Inhibition mm % mm % Wild type 4.1 ± 1.1 1.1 ± 0.2 74 13.9 ± 1.8 14.1 ± 2.3 −1 iaaL 2.0 ± 0.6 1.1 ± 0.3 45 16.9 ± 2.3 14.7 ± 2.1 13 axr1-3 1.7 ± 0.3 1.0 ± 0.1 37 15.6 ± 2.0 15.7 ± 2.1 −1 axr1-12 1.4 ± 0.3 1.1 ± 0.2 22 7.7 ± 2.1 3.4 ± 1.2 56 det2 0.8 ± 0.2 0.5 ± 0.1 40 2.9 ± 0.9 2.9 ± 0.6 −3 eto1-1 4.3 ± 1.0 1.3 ± 0.3 70 7.4 ± 1.3 5.7 ± 1.2 22 etr1 4.4 ± 0.8 1.3 ± 0.3 72 19.0 ± 2.5 14.7 ± 3.2 23
