Article Figures & Data
Figures
- Fig. 1.
Morphological comparison of tobacco lines ID8, ID9, and IR19. Left, Morphologically normal line ID8 21 d after subculture. Top right, Morphologically aberrant line ID9 21 d after subculture. Bottom right, Morphologically aberrant line IR19 21 d after subculture. The plants were grown in tissue culture on solid Murashige-Skoog medium containing 150 mg L−1kanamycin but no CuSO4.
- Fig. 2.
Comparison of leaf senescence in Cu-GUS and Cu-ipt tobacco transformants following treatment with 50 μm CuSO4. Cu-GUS (left) and Cu-ipt (right) plants growing in vitro following 97 d of treatment with 50 μm CuSO4.
- Fig. 3.
Comparison of growth patterns in Cu-GUS and Cu-ipt tobacco transformants following treatment with Cu. Left, Representative plant from Cu-GUS line GD11 following 30 d of treatment with Cu. Middle and right, Representative plants from Cu-ipt line ID8 following 30 d of treatment with Cu.
Tables
- Table I.
Cytokinins detected in the leaf tissue of Cu-ipt tobacco lines ID8 and ID9 under noninductive conditions
Cytokinin ID8 ID9 pmol g−1fresh wt ZR nd-a 134.4 DZR Trace-b Trace iPA Trace 9.6 ZOG Trace 96.4 ZROG nd 46.9 ZNT Trace 28.0 Total Trace 315.3 Values have been corrected for losses during purification and for differential cross-reactivity of the antibodies. The detection limit for ZR with clone 16 was 0.6 pmol and for iPA with clone 12 it was 0.8 pmol.
- Table II.
Comparison of the cytokinins detected in the leaf tissue of Cu-ipt tobacco line ID13 following in vitro treatment with water (−CuSO4) or Cu (+CuSO4).
Cytokinin −CuSO4 +CuSO4 pmol g−1 fresh wt Z nd1-a 27.5 DZR nd 52.2 iP Trace1-b 40.5 ZOG nd 11.8 Total Trace 132.0 Values have been corrected for losses during purification and for differential cross-reactivity of the antibodies. The detection limit for ZR with clone 16 was 0.6 pmol and for iPA with clone 12 it was 0.8 pmol.
- Table III.
Growth patterns of Cu-GUS and Cu-ipt tobacco from the in vivo experiment following 30 d of exposure to Cu
Strain Whole Plant Lateral Buds Node No.2-a Height Leaves No. Length Leaves cm no. cm no. Cu-GUS 14.5 (0.76)a 8.6 (0.47)a 1.2 (0.25)a 4.5 (0.64)a 2.1 (0.17)a 4.1 (0.57)a Cu-ipt 14.9 (0.62)a 11.8 (0.39)b 3.3 (0.21)b 7.0 (0.52)b 2.7 (0.12)b 5.6 (0.41)a P > 0.05 P < 0.0001 P < 0.0001 P = 0.0009 P = 0.0083 P > 0.05 Data presented are the estimates of the least-squares means of the morphological characteristics measured. ses are in parentheses. Those figures in each column followed by a different letter are significantly different at P ≤ 0.05.
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↵F2-a The node from which axillary buds were released (nodes were numbered upward from the base).
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