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Research ArticleCELL BIOLOGY AND SIGNAL TRANSDUCTION
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Arabidopsis Rho-Related GTPases: Differential Gene Expression in Pollen and Polar Localization in Fission Yeast

Hai Li, Guang Wu, Doreen Ware, Keith R. Davis, Zhenbiao Yang
Hai Li
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Guang Wu
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Doreen Ware
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Keith R. Davis
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Zhenbiao Yang
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Published October 1998. DOI: https://doi.org/10.1104/pp.118.2.407

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  • Copyright © 1998 American Society of Plant Physiologists

Abstract

The Rho small GTP-binding proteins are versatile, conserved molecular switches in eukaryotic signal transduction. Plants contain a unique subfamily of Rho-GTPases called Rop (Rho-related GTPases fromplants). Our previous studies involving injection of antibodies indicated that the pea Rop GTPase Rop1Ps is critical for pollen tube growth. In this study we show that overexpression of an apparent Arabidopsis ortholog of Rop1Ps,Rop1At, induces isotropic cell growth in fission yeast (Schizosaccharomyces pombe) and that green fluorescence protein-tagged Rop1At displays polar localization to the site of growth in yeast. We found that Rop1At and two other Arabidopsis Rops, Rop3At and Rop5At, are all expressed in mature pollen. All three pollen Rops fall into the same subgroup as Rop1Ps and diverge from those Rops that are not expressed in mature pollen, suggesting a coupling of the structural conservation of Rop GTPases to their gene expression in pollen. However, pollen-specific transcript accumulation forRop1At is much higher than that forRop3At and Rop5At. Furthermore,Rop1At is specifically expressed in anthers, whereasRop3At and Rop5At are also expressed in vegetative tissues. In transgenic plants containing theRop1At promoter:GUS fusion gene, GUS is specifically expressed in mature pollen and pollen tubes. We propose that Rop1At may play a predominant role in the regulation of polarized cell growth in pollen, whereas its close relatives Rop3At and Rop5At may be functionally redundant to Rop1At in pollen.

  • Abbreviations:

    DAPI
    4′,6-diamidino-phenylindole
    GFP
    green fluorescent protein
    RT
    reverse transcriptase
    • Received March 11, 1998.
    • Accepted June 22, 1998.
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    Arabidopsis Rho-Related GTPases: Differential Gene Expression in Pollen and Polar Localization in Fission Yeast
    Hai Li, Guang Wu, Doreen Ware, Keith R. Davis, Zhenbiao Yang
    Plant Physiology Oct 1998, 118 (2) 407-417; DOI: 10.1104/pp.118.2.407

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    Arabidopsis Rho-Related GTPases: Differential Gene Expression in Pollen and Polar Localization in Fission Yeast
    Hai Li, Guang Wu, Doreen Ware, Keith R. Davis, Zhenbiao Yang
    Plant Physiology Oct 1998, 118 (2) 407-417; DOI: 10.1104/pp.118.2.407
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    Plant Physiology: 118 (2)
    Plant Physiology
    Vol. 118, Issue 2
    Oct 1998
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    More in this TOC Section

    • The Cell Wall of the Arabidopsis Pollen Tube—Spatial Distribution, Recycling, and Network Formation of Polysaccharides
    • Systems Dynamic Modeling of a Guard Cell Cl− Channel Mutant Uncovers an Emergent Homeostatic Network Regulating Stomatal Transpiration
    • Architecture-Based Multiscale Computational Modeling of Plant Cell Wall Mechanics to Examine the Hydrogen-Bonding Hypothesis of the Cell Wall Network Structure Model
    Show more CELL BIOLOGY AND SIGNAL TRANSDUCTION

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