Skip to main content

Main menu

  • Authors
  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
    • Focus Collections
    • Classics Collection
    • Upcoming Focus Issues
  • Submit a Manuscript
  • Advertisers
  • About
    • About the Journal
    • Editorial Board and Staff
  • Subscribers
  • Librarians
  • More
    • Alerts
    • Contact Us
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Plant Cell Teaching Tools
    • ASPB
    • Plantae

User menu

  • My alerts
  • Log in

Search

  • Advanced search
Plant Physiology
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Plant Cell Teaching Tools
    • ASPB
    • Plantae
  • My alerts
  • Log in
Plant Physiology

Advanced Search

  • Authors
  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
    • Focus Collections
    • Classics Collection
    • Upcoming Focus Issues
  • Submit a Manuscript
  • Advertisers
  • About
    • About the Journal
    • Editorial Board and Staff
  • Subscribers
  • Librarians
  • More
    • Alerts
    • Contact Us
  • Follow plantphysiol on Twitter
  • Visit plantphysiol on Facebook
  • Visit Plantae
Research ArticleBIOCHEMISTRY AND MACROMOLECULAR STRUCTURE
You have accessRestricted Access

Photosynthetic and Heterotrophic Ferredoxin Isoproteins Are Colocalized in Fruit Plastids of Tomato

Koh Aoki, Miyuki Yamamoto, Keishiro Wada
Koh Aoki
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Miyuki Yamamoto
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Keishiro Wada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site

Published October 1998. DOI: https://doi.org/10.1104/pp.118.2.439

  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading
  • Copyright © 1998 American Society of Plant Physiologists

Abstract

Fruit tissues of tomato (Lycopersicon esculentum Mill.) contain both photosynthetic and heterotrophic ferredoxin (FdA and FdE, respectively) isoproteins, irrespective of their photosynthetic competence, but we did not previously determine whether these proteins were colocalized in the same plastids. In isolated fruit chloroplasts and chromoplasts, both FdA and FdE were detected by immunoblotting. Colocalization of FdA and FdE in the same plastids was demonstrated using double-staining immunofluorescence microscopy. We also found that FdA and FdE were colocalized in fruit chloroplasts and chloroamyloplasts irrespective of sink status of the plastid. Immunoelectron microscopy demonstrated that FdA and FdE were randomly distributed within the plastid stroma. To investigate the significance of the heterotrophic Fd in fruit plastids, Glucose 6-phosphate dehydrogenase (G6PDH) activity was measured in isolated fruit and leaf plastids. Fruit chloroplasts and chromoplasts showed much higher G6PDH activity than did leaf chloroplasts, suggesting that high G6PDH activity is linked with FdE to maintain nonphotosynthetic production of reducing power. This result suggested that, despite their morphological resemblance, fruit chloroplasts are functionally different from their leaf counterparts.

  • Abbreviations:

    DAPI
    4′,6-diamidino-2-phenylindole
    FITC
    fluorescein isothiocyanate
    FNR
    Fd-NADP+oxidoreductase
    G6PDH
    Glc 6-phosphate dehydrogenase
    Glc6P
    Glc 6-phosphate
    OPP
    oxidative pentose phosphate pathway
    TRITC
    tetramethyl-rhodamine B isothiocyanate
    • Received March 16, 1998.
    • Accepted June 23, 1998.
    View Full Text
    PreviousNext
    Back to top

    Table of Contents

    Print
    Download PDF
    Article Alerts
    Sign In to Email Alerts with your Email Address
    Email Article

    Thank you for your interest in spreading the word on Plant Physiology.

    NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

    Enter multiple addresses on separate lines or separate them with commas.
    Photosynthetic and Heterotrophic Ferredoxin Isoproteins Are Colocalized in Fruit Plastids of Tomato
    (Your Name) has sent you a message from Plant Physiology
    (Your Name) thought you would like to see the Plant Physiology web site.
    Citation Tools
    Photosynthetic and Heterotrophic Ferredoxin Isoproteins Are Colocalized in Fruit Plastids of Tomato
    Koh Aoki, Miyuki Yamamoto, Keishiro Wada
    Plant Physiology Oct 1998, 118 (2) 439-449; DOI: 10.1104/pp.118.2.439

    Citation Manager Formats

    • BibTeX
    • Bookends
    • EasyBib
    • EndNote (tagged)
    • EndNote 8 (xml)
    • Medlars
    • Mendeley
    • Papers
    • RefWorks Tagged
    • Ref Manager
    • RIS
    • Zotero
    Request Permissions
    Share
    Photosynthetic and Heterotrophic Ferredoxin Isoproteins Are Colocalized in Fruit Plastids of Tomato
    Koh Aoki, Miyuki Yamamoto, Keishiro Wada
    Plant Physiology Oct 1998, 118 (2) 439-449; DOI: 10.1104/pp.118.2.439
    del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
    • Tweet Widget
    • Facebook Like
    • Google Plus One

    Jump to section

    • Article
      • Abstract
      • MATERIALS AND METHODS
      • RESULTS
      • DISCUSSION
      • ACKNOWLEDGMENTS
      • Footnotes
      • Abbreviations:
      • LITERATURE  CITED
    • Figures & Data
    • Info & Metrics
    • PDF

    In this issue

    Plant Physiology: 118 (2)
    Plant Physiology
    Vol. 118, Issue 2
    Oct 1998
    • Table of Contents
    • About the Cover
    • Index by author
    View this article with LENS

    More in this TOC Section

    • A Tomato Peroxidase Involved in the Synthesis of Lignin and Suberin
    • Metabolic Relations of Inositol 3,4,5,6-Tetrakisphosphate Revealed by Cell Permeabilization. Identification of Inositol 3,4,5,6-Tetrakisphosphate 1-Kinase and Inositol 3,4,5,6-Tetrakisphosphate Phosphatase Activities in Mesophyll Cells
    • The Plastidic Phosphoglucomutase from Arabidopsis. A Reversible Enzyme Reaction with an Important Role in Metabolic Control
    Show more BIOCHEMISTRY AND MACROMOLECULAR STRUCTURE

    Similar Articles

    Our Content

    • Home
    • Current Issue
    • Plant Physiology Preview
    • Archive
    • Focus Collections
    • Classic Collections
    • The Plant Cell
    • Plant Direct
    • Plantae
    • ASPB

    For Authors

    • Instructions
    • Submit a Manuscript
    • Editorial Board and Staff
    • Policies
    • Recognizing our Authors

    For Reviewers

    • Instructions
    • Journal Miles
    • Policies

    Other Services

    • Permissions
    • Librarian resources
    • Advertise in our journals
    • Alerts
    • RSS Feeds

    Copyright © 2019 by The American Society of Plant Biologists

    Powered by HighWire