Article Figures & Data
Figures
- Fig. 1.
Five-week-old dark-grown seedlings of P. sylvestris. Some cotyledons were removed to expose the primary needles (arrow). Bar = 5 mm.
- Fig. 7.
Difference fluorescence spectra recorded after a flash (flash-minus-dark; curve a) and after 30 s of darkness (30-s dark-minus-flash; curve b).
- Fig. 2.
The 77 K fluorescence spectra during the development of dark-grown primary needles. Excitation wavelength: 440 nm. The spectra were normalized to their fluorescence emission maximum. A, P. jeffreyi. Curve a, 3 weeks old; curve b, 4 weeks old; curve c, 5 weeks old. B, P. sylvestris. Curve a, 5 weeks old; curve b, 6 weeks old; curve c, 7 weeks old.
- Fig. 3.
The 77 K fluorescence spectra of dark-grown primary needles of 6-week-old of P. sylvestris recorded with excitation wavelengths at 440 nm (curve a), 450 nm (curve b), 460 nm (curve c), and 470 nm (curve d). The spectra were normalized at 735 nm (A) and on the nonphotoactive Pchlide a fluorescence emission maximum (B). Top left, The 4th-derivative spectra of emission spectra recorded under 440 nm (solid line) and 470 nm (dashed line).
- Fig. 4.
Gaussian deconvolution of the spectrum recorded with excitation at 440 nm (top) and 470 nm (bottom) excitation lights. The difference between the Gaussian sum and the original data is presented in each case.
- Fig. 5.
The 77 K fluorescence excitation spectra at 630 nm (curve a) and 654 nm (curve b). Bottom, Fourth-order-derivative spectra. The excitation was set at 440 nm.
- Fig. 8.
The 77 K fluorescence spectra recorded with excitation at 470 nm. Curve a, Before the illumination (— – — –); curve b, after a saturating flash (– – – – –); curve c, after 30 s of darkness and the saturating flash (— — —); curve d, after 30 min of darkness and the saturating flash (———). Top left, Corresponding second-derivative calculations.
- Fig. 6.
The 77 K fluorescence spectra of 7-week-old dark-grown pine (P. sylvestris) primary needles cultivated in the absence (a) or presence (b) of norflurazon. The spectra were not normalized.
Tables
Pigment logk′-a Concentration pmol/plant Chlidea −0.53 86.86 Pchlidea −0.32 5.01 trans-Neoxanthin −0.03 58.13 trans-Violaxanthin 0.07 168.81 lutein-5,6-epoxide 0.22 87.92 Pheophytin b 0.33 ND-b trans-Lutein 0.41 340.85 Chlb 0.72 20.66 Chlide atetrahydrogeranylgeraniol 0.83 183.65 Chlide aphytol 0.86 100.20 α-Carotene 1.04 4.42 β-Carotene 1.05 30.45 Pheophytina 1.08 16.31 Chla/b -c 10.35 Xanthophyll/carotenoid 18.8 Carotenoid/Chl 0.34 Solvent Absorbance Maximum in the Soret Region (400–500 nm) References Pchlide a Chlide a MV DV MV DV Acetone — 437.7 431.0 436.0 Jeffrey et al. (1997) HPLC eluent 431.0 — 432.0 — Schoefs et al. (1995b) — 440.0 431.0 — This study - Table III.
Characteristics of the different components found by Gaussian deconvolutions of the fluorescence spectra and the percentage of each component in the emision spectra recorded under the 440-nm and 470-nm excitation light
Component Gaussian Maximum Half-Bandwidth Percentage of Total Area Pchlide Type Excitation at 440 nm Excitation at 470 nm nm % G622 621.89 ± 0.80 15.52 ± 2.23 15 1 ? G627 626.96 ± 0.56 16.90 ± 0.98 28 7 Pchlide photoinactive G632 631.95 ± 1.15 18.11 ± 1.56 28 12 Pchlide photoinactive G638 637.25 ± 1.34 18.08 ± 1.46 15 18 Pchlide photoinactive G643 643.78 ± 1.05 16.53 ± 3.01 5 19 Pchlide photoactive G651 650.48 ± 0.88 18.29 ± 1.18 4 20 Pchlide photoinactive G656 656.14 ± 0.80 19.50 ± 1.77 5 23 Pchlide photoactive
