Skip to main content

Main menu

  • For Authors
    • Submit a Manuscript
    • Instructions for Authors
  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
    • Focus Collections
    • Classics Collection
    • Upcoming Focus Issues
  • Advertisers
  • About
    • About the Journal
    • Editorial Board and Staff
  • Subscribers
  • Librarians
  • More
    • Alerts
    • Contact Us
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Plant Cell Teaching Tools
    • ASPB
    • Plantae

User menu

  • My alerts
  • Log in

Search

  • Advanced search
Plant Physiology
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Plant Cell Teaching Tools
    • ASPB
    • Plantae
  • My alerts
  • Log in
Plant Physiology

Advanced Search

  • For Authors
    • Submit a Manuscript
    • Instructions for Authors
  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
    • Focus Collections
    • Classics Collection
    • Upcoming Focus Issues
  • Advertisers
  • About
    • About the Journal
    • Editorial Board and Staff
  • Subscribers
  • Librarians
  • More
    • Alerts
    • Contact Us
  • Follow plantphysiol on Twitter
  • Visit plantphysiol on Facebook
  • Visit Plantae
Research ArticleGROWTH AND DEVELOPMENT
You have accessRestricted Access

Inhibition of Auxin Movement from the Shoot into the Root Inhibits Lateral Root Development in Arabidopsis

Robyn C. Reed, Shari R. Brady, Gloria K. Muday
Robyn C. Reed
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Shari R. Brady
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gloria K. Muday
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site

Published December 1998. DOI: https://doi.org/10.1104/pp.118.4.1369

  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Article Figures & Data

Figures

  • Tables
  • Fig. 1.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Fig. 1.

    Comparison of lateral root development with localized application of control agar (left) or agar containing 100 μm NPA (right) to the root-shoot junction. Roots were grown for 4 d after application of agar.

  • Fig. 2.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Fig. 2.

    The effect of NPA application at several positions on the Arabidopsis primary root. Five-day-old Arabidopsis seedlings were treated with a line of agar containing 100 μm NPA at several positions along the root. The root was divided into 10-mm zones, with zone 1 beginning at the root-shoot junction. The lines of agar were placed above zone 1, 2, or 3, as indicated. The number and density of lateral roots were determined after 3 d of growth. The data were analyzed by Student's t test, and P values were determined for each sample compared with the controls and are indicated with asterisks (*P ≥ 0.015 and ≤ 0.038; **P ≤ 0.005).

Tables

  • Figures
    • View popup
    Table I.

    Effects of localized NPA application on lateral root development and root length

    [NPA]Lateral Root No.Root LengthLateral Root Density-a
    μm mm
    0.015.9  ± 1.048.6  ± 1.40.33  ± 0.02
    5.012.3  ± 0.847.2  ± 0.70.26  ± 0.02-b
    10.010.4  ± 1.145.9  ± 1.10.23  ± 0.02-b
    20.08.6  ± 0.739.0  ± 1.70.22  ± 0.20-b
    50.06.4  ± 0.645.0  ± 0.90.14  ± 0.02-b
    100.01.0  ± 0.338.7  ± 1.70.03  ± 0.00-b

    Four-day-old seedlings were treated by application of agar containing NPA at the indicated concentrations to the root-shoot junction. After 6 additional d of growth, lateral roots were counted and the length of the primary roots was measured. The reported values are averages ± se of 10 plants.

      • ↵F0-a The lateral root density was calculated by dividing the number of lateral roots by the primary root length for each plant, and is reported as the number of lateral roots per millimeter of primary root. The average ± se of the lateral root density calculated for each root is reported.

      • ↵F0-b Lateral root density was significantly reduced by all concentrations of NPA (P ≤ 0.007).

      • View popup
      Table II.

      Effects of localized NPA application at the root-shoot junction on free IAA concentration

      TreatmentFree IAA Concentration1-aLateral Root No.1-b
      ng/g fresh wt
      Control agar25.8  ± 4.715.5  ± 0.8
      NPA agar17.6  ± 2.25.9  ± 0.5

      Seven- or 8-d-old seedlings were treated by application of agar with or without NPA to the root-shoot junction. After 3 additional d of growth, roots were harvested and the free IAA concentration was determined by GC-SIM-MS or the number of lateral roots was determined.

        • ↵F1-a The reduction in free IAA concentration was statistically significant using a Mann-Whitney U test (P = 0.04).

        • ↵F1-b The reduction in lateral root number was statistically significant using Student's t test (P ≤ 0.001).

        • View popup
        Table III.

        Effects of localized NPA application on [3H]IAA movement

        Treatment[3H]IAA Transported
        cpmfmol
        Control agar1501  ± 17561.4  ± 7.1
        NPA agar1124  ± 5246.0  ± 2.1

        Agar blocks containing [3H]IAA were applied to 4-d-old seedlings with and without NPA-containing blocks, and radioactivity in the roots was determined after 24 h. The reported values are averages ± se of four replicates, each containing five plants.

          • F2-a Using a Mann-Whitney U test, the differences in IAA are statistically significant (P ≤ 0.04).

          • View popup
          Table IV.

          Effects of application of NPA to the upper and lower halves of seedling roots

          AgarLateral Root No.Lateral Root Density3-aChange in Length3-b
          mm
          Control3-c 4.4  ± 0.60.46  ± 0.07
          Control3-d 5.3  ± 1.10.14  ± 0.0330.2  ± 1.0
          NPA3-c 1.7  ± 0.50.18  ± 0.053-e
          Control3-d 0.1  ± 0.10.00  ± 0.003-e 23.5  ± 1.0
          Control3-c 4.4  ± 0.50.49  ± 0.06
          NPA3-d 1.3  ± 0.60.05  ± 0.023-e 12.1  ± 0.7

          Six-day-old seedlings were transferred to plates containing 10 μm NPA in the upper or lower half, as indicated. After 4 additional d of growth in the light, lateral roots in the upper and lower halves were counted and new primary root growth was measured. The reported values are averages ± se of 10 plants.

            • ↵F3-a The lateral root density was calculated by dividing the number of lateral roots by the primary root length for each plant, and is reported as the number of lateral roots per millimeter of primary root.

            • ↵F3-b The amount of elongation of the primary root after transfer to the indicated plates.

            • ↵F3-c The compound in agar and the lateral root growth on the upper half of the plate is shown.

            • ↵F3-d The compound in agar and the lateral root growth on the lower half of the plate is shown.

            • ↵F3-e Lateral root density decreased significantly (P ≤ 0.002) as judged by Student's ttest.

            • View popup
            Table V.

            Reversal of NPA-induced lateral root inhibition by localized IAA application

            Agar4-aLateral Root No.Lateral Root Density
            UpperLower
            ControlControl6.7  ± 0.850.19  ± 0.02
            IAAControl10.0  ± 0.560.43  ± 0.03
            ControlNPA0.33  ± 0.140.02  ± 0.01
            NPAIAA4.25  ± 0.730.24  ± 0.04
            IAANPA1.72  ± 0.460.08  ± 0.02

            After the indicated treatments, lateral root number and root length were determined after 4 d of additional growth in the light. The reported values are averages ± se of 10 plants.

              • ↵F4-a Two, thin (1-mm), parallel lines of agar either with or without 10 μm IAA and 100 μm NPA were applied to the root-shoot junction of seedlings that were 4 d old. This technique allowed for the simultaneous application of two compounds to seedlings. Although both lines were over the root-shoot junction of the seedlings, one line was closer to the plant shoot (the Upper line) and the other was closer to the root (the Lower line).

              • View popup
              Table VI.

              Effects of application of IAA to the upper and lower halves of roots

              AgarLateral Root No.Lateral Root Density5-aChange in Length5-b
              mm
              Control5-c 4.6  ± 0.40.62  ± 0.06
              Control5-d 5.3  ± 0.90.14  ± 0.0243.7  ± 1.8
              IAA5-c 7.2  ± 0.50.98  ± 0.275-e
              Control5-d 9.1  ± 1.80.33  ± 0.065-f 37.8  ± 1.8
              Control5-c 4.3  ± 0.40.47  ± 0.065-e
              IAA5-d 10.1  ± 0.90.30  ± 0.035-f 42.4  ± 2.5

              Six-day-old seedlings were transferred to plates containing 0.1 μm IAA in the upper or lower half, as indicated. After 4 additional d of growth in the light, lateral roots in the upper and lower halves were counted and new primary root growth was measured. The reported values are averages ± se of 10 plants.

                • ↵F5-a The lateral root density was calculated by dividing the number of lateral roots by the primary root length for each plant, and is reported as the number of lateral roots per millimeter of primary root.

                • ↵F5-b The amount of elongation of the primary root after transfer to the indicated plates.

                • ↵F5-c The compound in agar and the lateral root development on the upper half of the plate is shown.

                • ↵F5-d The compound in agar and the lateral root development on the lower half of the plate is shown.

                • ↵F5-e These values are not statistically different from the control values (P ≥ 0.06).

                • ↵F5-f These values are statistically different from the control values (P ≤ 0.004).

                • View popup
                Table VII.

                Effects of excision and localized IAA application on lateral root development

                Treatment[IAA]Lateral Root No.Root LengthLateral Root Density6-a
                μm mm
                Intact plants6-b 0.028.6  ± 2.351.9  ± 3.10.56  ± 0.04
                Shoot excised6-b 0.06.4  ± 0.445.7  ± 1.90.14  ± 0.01
                Root tip excised6-b 0.020.8  ± 0.827.7  ± 0.80.76  ± 0.03
                Shoot excised6-c 0.06.0  ± 1.042.4  ± 2.10.14  ± 0.02
                Shoot excised0.16.3  ± 0.946.7  ± 2.00.13  ± 0.02
                Shoot excised1.09.3  ± 1.243.5  ± 1.60.21  ± 0.03
                Shoot excised10.013.1  ± 1.336.7  ± 1.50.36  ± 0.03

                Plants were treated by shoot or root-tip excision and application of agar containing IAA at the indicated concentrations to the root-shoot junction. After 3 additional d of growth, lateral roots were counted and the length of the primary roots was measured. The reported values are averages ± se of 10 plants.

                  • ↵F6-a The lateral root density was calculated by dividing the number of lateral roots by the primary root length for each plant, and is reported as the number of lateral roots per millimeter of primary root.

                  • ↵F6-b Five-day-old seedlings were treated and grown for 3 additional d in the light.

                  • ↵F6-c Eight-day-old seedlings were treated and grown for 3 additional d in the dark.

                  • View popup
                  Table VIII.

                  Effects of localized IAA application on lateral root development in dark-grown plants

                  [IAA]Lateral Root No.Root LengthLateral Root Density7-a
                  μm mm
                  0.00.1  ± 0.133.5  ± 2.50.00  ± 0.00
                  0.10.7  ± 0.431.7  ± 1.40.02  ± 0.01
                  1.02.1  ± 0.427.6  ± 4.10.09  ± 0.027-b
                  5.06.0  ± 0.515.4  ± 0.80.40  ± 0.027-b
                  10.09.3  ± 0.713.0  ± 0.60.70  ± 0.077-b

                  Seven-day-old seedlings were treated by application of agar containing IAA at the indicated concentrations to the root-shoot junction. After 3 additional d of growth, lateral roots were counted and the length of the primary roots was measured. The reported values are averages ± se of 10 plants.

                    • ↵F7-a The lateral root density was calculated by dividing the number of lateral roots by the primary root length for each plant, and is reported as the number of lateral roots per millimeter of primary root.

                    • ↵F7-b The increase in lateral root density was statistically significant, as judged by Student'st test (P ≤ 0.0008).

                  PreviousNext
                  Back to top

                  Table of Contents

                  Print
                  Download PDF
                  Email Article

                  Thank you for your interest in spreading the word on Plant Physiology.

                  NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

                  Enter multiple addresses on separate lines or separate them with commas.
                  Inhibition of Auxin Movement from the Shoot into the Root Inhibits Lateral Root Development in Arabidopsis
                  (Your Name) has sent you a message from Plant Physiology
                  (Your Name) thought you would like to see the Plant Physiology web site.
                  CAPTCHA
                  This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
                  Citation Tools
                  Inhibition of Auxin Movement from the Shoot into the Root Inhibits Lateral Root Development in Arabidopsis
                  Robyn C. Reed, Shari R. Brady, Gloria K. Muday
                  Plant Physiology Dec 1998, 118 (4) 1369-1378; DOI: 10.1104/pp.118.4.1369

                  Citation Manager Formats

                  • BibTeX
                  • Bookends
                  • EasyBib
                  • EndNote (tagged)
                  • EndNote 8 (xml)
                  • Medlars
                  • Mendeley
                  • Papers
                  • RefWorks Tagged
                  • Ref Manager
                  • RIS
                  • Zotero
                  Request Permissions
                  Share
                  Inhibition of Auxin Movement from the Shoot into the Root Inhibits Lateral Root Development in Arabidopsis
                  Robyn C. Reed, Shari R. Brady, Gloria K. Muday
                  Plant Physiology Dec 1998, 118 (4) 1369-1378; DOI: 10.1104/pp.118.4.1369
                  del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
                  • Tweet Widget
                  • Facebook Like
                  • Google Plus One

                  Jump to section

                  • Article
                    • Abstract
                    • MATERIALS AND METHODS
                    • RESULTS AND DISCUSSION
                    • CONCLUSIONS
                    • ACKNOWLEDGMENTS
                    • Footnotes
                    • Abbreviations:
                    • LITERATURE  CITED
                  • Figures & Data
                  • Info & Metrics
                  • PDF

                  In this issue

                  Plant Physiology: 118 (4)
                  Plant Physiology
                  Vol. 118, Issue 4
                  Dec 1998
                  • Table of Contents
                  • About the Cover
                  • Index by author
                  View this article with LENS

                  More in this TOC Section

                  • The Expression of D-Cyclin Genes Defines Distinct Developmental Zones in Snapdragon Apical Meristems and Is Locally Regulated by the Cycloidea Gene
                  • Independent Action of ELF3 and phyB to Control Hypocotyl Elongation and Flowering Time
                  • Abscisic Acid Inhibition of Radicle Emergence But Not Seedling Growth Is Suppressed by Sugars
                  Show more GROWTH AND DEVELOPMENT

                  Similar Articles

                  Our Content

                  • Home
                  • Current Issue
                  • Plant Physiology Preview
                  • Archive
                  • Focus Collections
                  • Classic Collections
                  • The Plant Cell
                  • Plant Direct
                  • Plantae
                  • ASPB

                  For Authors

                  • Instructions
                  • Submit a Manuscript
                  • Editorial Board and Staff
                  • Policies
                  • Recognizing our Authors

                  For Reviewers

                  • Instructions
                  • Journal Miles
                  • Policies

                  Other Services

                  • Permissions
                  • Librarian resources
                  • Advertise in our journals
                  • Alerts
                  • RSS Feeds

                  Copyright © 2021 by The American Society of Plant Biologists

                  Powered by HighWire