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Research ArticleBIOCHEMISTRY AND MACROMOLECULAR STRUCTURE
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Purification and Characterization of a NADPH-Dependent Aldehyde Reductase from Mung Bean That Detoxifies Eutypine, a Toxin from Eutypa lata 1

Ségolène Colrat, Alain Latché, Monique Guis, Jean-Claude Pech, Mondher Bouzayen, Jean Fallot, Jean-Paul Roustan
Ségolène Colrat
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Alain Latché
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Monique Guis
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Jean-Claude Pech
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Mondher Bouzayen
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Jean Fallot
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Jean-Paul Roustan
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Published February 1999. DOI: https://doi.org/10.1104/pp.119.2.621

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  • Copyright © 1999 American Society of Plant Physiologists

Abstract

Eutypine (4-hydroxy-3-[3-methyl-3-butene-1-ynyl] benzaldehyde) is a toxin produced by Eutypa lata, the causal agent of eutypa dieback in the grapevine (Vitis vinifera). Eutypine is enzymatically converted by numerous plant tissues into eutypinol (4-hydroxy-3-[3-methyl-3-butene-1-ynyl] benzyl alcohol), a metabolite that is nontoxic to grapevine. We report a four-step procedure for the purification to apparent electrophoretic homogeneity of a eutypine-reducing enzyme (ERE) from etiolated mung bean (Vigna radiata) hypocotyls. The purified protein is a monomer of 36 kD, uses NADPH as a cofactor, and exhibits aK m value of 6.3 μm for eutypine and a high affinity for 3- and 4-nitro-benzaldehyde. The enzyme failed to catalyze the reverse reaction using eutypinol as a substrate. ERE detoxifies eutypine efficiently over a pH range from 6.2 to 7.5. These data strongly suggest that ERE is an aldehyde reductase that could probably be classified into the aldo-keto reductase superfamily. We discuss the possible role of this enzyme in eutypine detoxification.

  • Abbreviation:

    ERE
    eutypine-reducing enzyme
    • Received June 29, 1998.
    • Accepted October 19, 1998.
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    Purification and Characterization of a NADPH-Dependent Aldehyde Reductase from Mung Bean That Detoxifies Eutypine, a Toxin from Eutypa lata 1
    Ségolène Colrat, Alain Latché, Monique Guis, Jean-Claude Pech, Mondher Bouzayen, Jean Fallot, Jean-Paul Roustan
    Plant Physiology Feb 1999, 119 (2) 621-626; DOI: 10.1104/pp.119.2.621

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    Purification and Characterization of a NADPH-Dependent Aldehyde Reductase from Mung Bean That Detoxifies Eutypine, a Toxin from Eutypa lata 1
    Ségolène Colrat, Alain Latché, Monique Guis, Jean-Claude Pech, Mondher Bouzayen, Jean Fallot, Jean-Paul Roustan
    Plant Physiology Feb 1999, 119 (2) 621-626; DOI: 10.1104/pp.119.2.621
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    Plant Physiology: 119 (2)
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    Feb 1999
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