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Research ArticleBIOCHEMISTRY AND MACROMOLECULAR STRUCTURE
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Limonene Synthase, the Enzyme Responsible for Monoterpene Biosynthesis in Peppermint, Is Localized to Leucoplasts of Oil Gland Secretory Cells

Glenn Turner, Jonathan Gershenzon, Erik E. Nielson, John E. Froehlich, Rodney Croteau
Glenn Turner
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Jonathan Gershenzon
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Erik E. Nielson
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John E. Froehlich
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Rodney Croteau
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Published July 1999. DOI: https://doi.org/10.1104/pp.120.3.879

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  • Copyright © 1999 American Society of Plant Physiologists

Abstract

Circumstantial evidence based on ultrastructural correlation, specific labeling, and subcellular fractionation studies indicates that at least the early steps of monoterpene biosynthesis occur in plastids. (4S)-Limonene synthase, which is responsible for the first dedicated step of monoterpene biosynthesis in mint species, appears to be translated as a preprotein bearing a long plastidial transit peptide. Immunogold labeling using polyclonal antibodies raised to the native enzyme demonstrated the specific localization of limonene synthase to the leucoplasts of peppermint (Mentha× piperita) oil gland secretory cells during the period of essential oil production. Labeling was shown to be absent from all other plastid types examined, including the basal and stalk cell plastids of the secretory phase glandular trichomes. Furthermore, in vitro translation of the preprotein and import experiments with isolated pea chloroplasts were consistent in demonstrating import of the nascent protein to the plastid stroma and proteolytic processing to the mature enzyme at this site. These experiments confirm that the leucoplastidome of the oil gland secretory cells is the exclusive location of limonene synthase, and almost certainly the preceding steps of monoterpene biosynthesis, in peppermint leaves. However, succeeding steps of monoterpene metabolism in mint appear to occur outside the leucoplasts of oil gland cells.

  • Abbreviation:

    SSU
    small subunit of Rubisco
    • Received February 8, 1999.
    • Accepted March 28, 1999.
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    Limonene Synthase, the Enzyme Responsible for Monoterpene Biosynthesis in Peppermint, Is Localized to Leucoplasts of Oil Gland Secretory Cells
    Glenn Turner, Jonathan Gershenzon, Erik E. Nielson, John E. Froehlich, Rodney Croteau
    Plant Physiology Jul 1999, 120 (3) 879-886; DOI: 10.1104/pp.120.3.879

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    Limonene Synthase, the Enzyme Responsible for Monoterpene Biosynthesis in Peppermint, Is Localized to Leucoplasts of Oil Gland Secretory Cells
    Glenn Turner, Jonathan Gershenzon, Erik E. Nielson, John E. Froehlich, Rodney Croteau
    Plant Physiology Jul 1999, 120 (3) 879-886; DOI: 10.1104/pp.120.3.879
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    Plant Physiology: 120 (3)
    Plant Physiology
    Vol. 120, Issue 3
    Jul 1999
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    • Phytoene Desaturase Is Localized Exclusively in the Chloroplast and Up-Regulated at the mRNA Level during Accumulation of Secondary Carotenoids in Haematococcus pluvialis (Volvocales, Chlorophyceae)
    • Identification of a Hsp70 Recognition Domain within the Rubisco Small Subunit Transit Peptide
    • Cloning and Expression of Cytochrome P450 Enzymes Catalyzing the Conversion of Tyrosine to p-Hydroxyphenylacetaldoxime in the Biosynthesis of Cyanogenic Glucosides in Triglochin maritima
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