Article Figures & Data
Figures
- Fig. 1.
GA-biosynthetic pathways from GA12 showing the non-13- and early-13-hydroxylation pathways.
- Fig. 2.
GA 2-oxidase genomic clone from cv Alaska pea. cDNA sequence is shown in bold type; the deduced amino acid sequence is shown in italics. The missing nucleotide in sln cDNA is shaded. Primers used in PCR and two restriction sites for enzymes used in the Southern analysis are underlined. Sequences are registered under GenBank accession nos. AF101383 (SLN genomic clone),AF056935 (SLN cDNA), and AF101382 (slncDNA).
- Fig. 3.
Southern analysis of Progress No. 9 pea. The blot was probed with a PsGA2ox1 cDNA clone and washed sequentially at low (left) and high (right) stringency.
- Fig. 4.
RNA analysis of Progress No. 9 pea. A, Northern blot of poly(A+) RNA isolated from various organs (3 μg/lane). B, Slot blot of poly(A+) RNA from dissected seed 26 DAF (top) and whole seed (bottom) (1 μg/slot). Relative intensities were corrected for background and quantified on a phosphor imager. Band intensities for cotyledons and testae differed by 1,000-fold.
- Fig. 5.
RFLP analysis of slender backcross NGB6074 × (NGB6074 × I3). Progeny are arranged by size and segregate into two groups based on the length of stem between the first bract and first true leaf (shown in millimeters above each lane). Wild-type (tall) progeny are heterozygous for both alleles; slender progeny are homozygous for the mutant allele. Figure is a composite of two Southern blots probed with PsGA2ox1 cDNA.
Tables
Substrate Product Mass Spectra of Products m/z (% relative abundance) [2H2]GA20 GA29 M+508(100), 493(8), 479(5), 449(7), 391(8), 377(9), 305(21), 237(6), 209(33), 169(14) GA29-catabolite1-a M+520(100), 477(89), 447(7), 431(32), 311(10) [2H4]GA9 GA51 M+422(13), 407(11), 390(35), 374(9), 332(45), 300(31), 289(93), 288(95), 272(55), 24?(37), 229(100), 228(92), 202(22), 184(32) GA51-catabolite1-a M+434(100), 419(4), 390(9), 375(13), 357(9), 343(3), 315(35), 285(7), 269(12), 244(10), 225(3) [2H2]GA1 GA8 M+596(100), 581(7), 538(6), 506(3), 450(16), 381(7), 379(7), 331(3), 313(5), 283(5), 240(18), 209(20) [2H2]GA4 GA34 M+508(100), 476(30), 461(2), 388(5), 376(7), 357(10), 315(10), 290(13) -
↵F1-a As the trimethylsilyl enol.
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Fraction Relative Enzyme Concentration 100× 10× 1× GA20 6.4 6.9 24.5 GA29 66.6 79.5 74.0 GA29-catabolite 26.4 13.6 1.4 Lysate of bacteria expressing GA 2-oxidase was diluted with inactive control lysate and incubated overnight at 20°C with substrate and cofactors. Products were separated by HPLC. Results are expressed as percentages of total counts in substrate and product fractions.
