Article Figures & Data
Figures
- Fig. 1.
Perturbed auxin transport in mesocotyls ofrs2 mutant seedlings. Acropetal transport (black bars) and basipetal (white bars) transport of exogenously supplied C-14 IAA in wild-type and mutant (rs2-twd) seedlings. In wild-type plants, a significant difference is seen between the acropetal and basipetal measurements, demonstrating the presence of active polar basipetal transport mechanisms. In mutant plants, no significant difference is seen between basipetal and acropetal measurements, suggesting that the ability to basipetally transport auxin is significantly reduced in mutant tissue.
- Fig. 2.
Effects of PAT inhibitors on maize seedling growth. Dwarfism and twisting: A and C, Plants on the left have been germinated on control medium and allowed to grow in sterile pots for 2 weeks; plants on the right have been germinated in the presence of 28 μm TIBA and grown for the same time. B, The plant on the left is a wild-type sibling of the rs2 mutant plant shown on the right. Displaced ligule formation: D, Seedling leaf of an untreated wild-type plant. White arrow points to the ligule. Leaf twisting and aberrant ligular formation in a TIBA-treated wild-type plant (E) and in a rs2 mutant plant (F). White arrows point to the non-discrete ligular boundary. Hypertrophic vascularization: G, Vascular morphology of a lateral vein in an untreated wild-type seedling. Vascular hypertrophy seen in a TIBA-treated plant (H) and in a rs2 mutant (I) plant. Xylem and phloem are labeled X and P, respectively. Gray lines indicate the edge of the phloem in each case. Bar = 30 μm.
- Fig. 3.
Precocious axillary bud development inrs2 mutant seedlings. A, Median section through the apical region of a 10-d-old wild-type seedling. Bar = 1 mm. B, Median section through the apical region of a 10-d-oldrs2 mutant seedling. Arrowheads point toward the axillary buds. Size bar = 1 mm. C, Number of axillary buds developed in 10-d-old wild-type (wt) and rs2 mutant seedlings.
- Fig. 4.
KNOX protein accumulation patterns in TIBA-treated wild-type plants. A, Median section through the apical region of a 10-d-old untreated wild-type seedling. B, Median section through the apical region of a 10-d-old TIBA-treated wild-type seedling. Arrows denote the position of axillary meristems. C, Transverse section through the apical region of a 10-d-old TIBA-treated wild-type seedling. Bars = 100 μm.
Tables
- Table I.
Phenotypes exhibited by wild-type plants treated with the auxin transport inhibitor TIBA
TIBA Control no. Agravitropic roots 28 0 Dwarfism 25 4 Twisted leaves/stem 15 0 Aberrant ligule 3 0 Feiled to germinate 5 2 Severe growth arrest 3 1 Seventy-six plants were grown, half on control medium and half on medium containing TIBA. The number of plants showing specific phenotypes is indicated.
