Research ArticleCELL BIOLOGY AND SIGNAL TRANSDUCTION

Calmodulin Activation of an Endoplasmic Reticulum-Located Calcium Pump Involves an Interaction with the N-Terminal Autoinhibitory Domain

Ildoo Hwang, Jeffrey F. Harper, Feng Liang, Heven Sze

Published January 2000. DOI: https://doi.org/10.1104/pp.122.1.157

Abstract

To investigate how calmodulin regulates a unique subfamily of Ca2+ pumps found in plants, we examined the kinetic properties of isoform ACA2 identified in Arabidopsis. A recombinant ACA2 was expressed in a yeast K616 mutant deficient in two endogenous Ca2+ pumps. Orthovanadate-sensitive45Ca2+ transport into vesicles isolated from transformants demonstrated that ACA2 is a Ca2+ pump. Ca2+ pumping by the full-length protein (ACA2-1) was 4- to 10-fold lower than that of the N-terminal truncated ACA2-2 (Δ2–80), indicating that the N-terminal domain normally acts to inhibit the pump. An inhibitory sequence (IC50 = 4 μm) was localized to a region within valine-20 to leucine-44, because a peptide corresponding to this sequence lowered the V max and increased theK m for Ca2+ of the constitutively active ACA2-2 to values comparable to the full-length pump. The peptide also blocked the activity (IC50 = 7 μm) of a Ca2+ pump (AtECA1) belonging to a second family of Ca2+ pumps. This inhibitory sequence appears to overlap with a calmodulin-binding site in ACA2, previously mapped between asparatate-19 and arginine-36 (J.F. Harper, B. Hong, I. Hwang, H.Q. Guo, R. Stoddard, J.F. Huang, M.G. Palmgren, H. Sze [1998] J Biol Chem 273: 1099–1106). These results support a model in which the pump is kept “unactivated” by an intramolecular interaction between an autoinhibitory sequence located between residues 20 and 44 and a site in the Ca2+ pump core that is highly conserved between different Ca2+ pump families. Results further support a model in which activation occurs as a result of Ca2+-induced binding of calmodulin to a site overlapping or immediately adjacent to the autoinhibitory sequence.

The importance of Ca2+ in signaling, growth, and development has long been recognized; however, the temporal and spatial regulation of Ca2+ levels in plant cells is poorly understood (Bush, 1995; Sanders et al., 1999). A variety of signals, such as drought and salinity, cause transient opening of Ca2+ channels in the plasma membrane (PM) and/ or endomembranes. A current model is that [Ca2+] fluctuations in microdomains of the cytosol are sensed and decoded to produce specific cellular and physiological responses (Sanders et al., 1999), such as in abscisic acid-induced closure of stomatal aperture (Ward et al., 1995). The frequency and pattern of cytosolic Ca2+transients are determined by the coordinate regulation of two opposing fluxes: Ca2+ influx via channels and Ca2+ efflux via active transporters. Ca2+-pumping ATPases comprise the bulk of the active transporters, and serve three major functions: (a) to lower cytosolic Ca2+; (b) to replenish Ca2+ sinks in the vacuole and endoplasmic reticulum (ER); and (c) to supply Ca2+ in endoluminal compartments for biochemical functions (Sanders et al., 1999).

In spite of the significant functions, little is known at the molecular level about the different Ca2+ pumps or their specific modes of regulation. Both biochemical and molecular studies provide evidence for two distinct families of Ca2+ pumps in plants often referred to as “ER-type” (or type IIA) and “PM-type” (or type IIB) (Geisler et al., 1999). ER-type Ca2+ pumps, like the Arabidopsis ECA1, are often localized on the ER, and are insensitive to calmodulin (Liang et al., 1997; Liang and Sze, 1998). PM-type Ca2+ pumps, however, are stimulated by calmodulin (Hwang et al., 1997).

Although calmodulin-stimulated Ca2+ pumps from plants are more closely related to animal plasma membrane Ca2+-ATPases in protein sequence, this type of pump in plants is localized on the vacuolar membrane (Askerlund, 1996,1997; Malmstrom et al., 1997), the ER (Hwang et al., 1997; Hong et al., 1999), and the plasma membrane (Rasi-Caldogno et al., 1992). At least two different Ca2+ pumps from plants had been purified by calmodulin-affinity chromatography. One protein of 111 kD was localized to the vacuole of cauliflower (Askerlund, 1996, 1997) and another protein of 133 kD (Bonza et al., 1998) from the PM of radish. Several plant genes encoding homologs of animal PM Ca2+ pumps have been isolated, including ACA1/PEA (Huang et al., 1993), ACA2 (Harper et al., 1998), and BCA1. Evidence indicates that BCA1 encodes a calmodulin-stimulated Ca2+ pump based on correspondence to peptide sequences from a purified vacuolar pump (Malmstrom et al., 1997).

An intriguing discovery about plant plasma membrane Ca2+-ATPase homologs is that BCA1 and ACA2 possess a regulatory domain at the N terminus instead of the C terminus (Malmstrom et al., 1997; Harper et al., 1998). We previously showed that the full-length ACA2 failed to restore growth on Ca2+-depleted medium of a yeast mutant harboring a disruption of its endogenous Ca2+ pumps. However, a truncated ACA2 (Δ2–80 residues) rescued the mutant phenotype and displayed constitutively active Ca2+-dependent ATPase activity (Harper et al., 1998). These results suggested that ACA2 encodes a Ca2+ pump.

In the present study we show that ACA2 can transport Ca2+, providing the first direct evidence (to our knowledge) that ACA2 is a Ca2+ pump. Furthermore, we determine the kinetic properties to identify the autoinhibitory sequence and to understand how the pump is regulated by calmodulin. The velocity of the full-length protein and its affinity for Ca2+ were increased by calmodulin to values equivalent to those observed for the de-regulated (N-terminally truncated) mutant in the absence of calmodulin. We also show that a peptide sequence derived from the N-terminal domain can inhibit the activity of the de-regulated pump. Our results support a model in which activation occurs as a result of calmodulin binding to a site overlapping or immediately adjacent to an autoinhibitory sequence located in the N-terminal domain, and thereby displacing its inhibitory interactions.

MATERIALS AND METHODS

Yeast Strains and Their Growth Media

Saccharomyces cerevisiae strains W303-1A (MATa, leu2, his3, ade2,trp1, and ura3) and K616 (MATa pmr1::HIS3 pmc1::TRP1 cnb1::LEU2, ura3) were used (Cunningham and Fink, 1994). Wild-type W303-1A and mutant K616 strains were grown for 24 h in standard yeast peptone dextrose medium before transformation. K616strain grows better in media supplemented with 10 mm CaCl2. After transformation with pYX plasmid constructs, transformants were selected on synthetic complete medium minus uracil (SC-URA). The medium consisted of 1 mmCa2+, 6.7 g/L yeast nitrogen base without amino acids, 2 g/L drop-out mix without uracil, and 2% (v/v) Glc as a carbon source (Rose et al., 1990).

cDNA and Constructs

Contructs used for the expression of a full-length ACA2 and truncated ACA2-2 (ΔP80) were previously described, pYX-ACA2-1 and pYX-ACA2-2, respectively (Harper et al., 1998). The vector used was pYX-112 (formerly distributed by Novagen, Madison, WI). ACA2 and ACA2-2 were expressed under the control of a strong constitutive promoter from triose phosphate isomerase. The pYX-112 vector contains URA3 as a selection marker.

Yeast Transformation and Growth

Wild-type and mutant strains of S. cerevisiae were transformed with pYX vector alone, pYX-ACA2-1, or pYX-ACA2-2 by the LiOAc/polyethylene glycol methods (Becker and Guarente, 1991) and selected for uracil prototrophy by plating on SC-URA medium. The Ura+ colonies were picked and grown for 2 to 3 d on SC-URA agar plates.

To measure growth, K616 transformants were grown overnight and the culture was suspended in 50 mL of SC-URA medium at pH 6.5 to an initialA 600 of 0.1. The medium contained 1 mm Ca2+, and free Ca2+ levels were controlled by adding varying amounts of EGTA. Growth was monitored by the change inA 600 of 0.8-mL samples for 24 h.

Isolation of Yeast Membranes

Transformants were inoculated into 20 mL of SC-URA medium and incubated overnight. The culture was diluted 10-fold into SC-URA medium and grown overnight to an A 600 of 1 to 1.8. The cells were pelleted at 4,000g for 5 min, washed with 10 mL of distilled water, and pelleted again. Membranes were isolated using the glass bead method (Serrano, 1988) with some modification (Liang and Sze, 1998). Cells were suspended in 10 mL of glass beads buffer (GBB) containing 10% (w/w) Suc, 25 mm4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)-KOH (pH 7.5), 2 mm EGTA, and 2 mmdithiothreitol, and pelleted. Typically 1- to 2-g cells were suspended in 4 to 5 mL of GBB plus 1 mm phenylmethylsulfonyl fluoride (PMSF), 0.1 mm N-tosyl-l-Phe chloromethyl ketone (TPCK), 10 mm benzamidine, 5 μg/mL pepstatin, and leupeptin.

Acid-washed glass beads (Sigma-Aldrich, St. Louis) were added to the meniscus, and cells were mixed on a vortex six times for 30 s each with intermittent chilling. The lysate was centrifuged at 5,000g for 5 min. The supernatant was layered onto a 20% and 45% (w/w) Suc step gradient containing 25 mmHEPES-KOH (pH 7.0), 1 mm dithiothreitol, 0.2 mm PMSF, and 5 mmbenzamidine, and centrifuged at 108,000g for 3 h. Membranes at the 20%:45% (w/w) Suc interface were collected (1.3–1.5 mL) and diluted with 10 mL of 10% (w/w) Suc in 25 mmHEPES-1,3-bis(Tris[hydroxymethyl]methylamino) propane (BTP) (pH 7.0), 1 mm PMSF, 0.1 mmTPCK, 10 mm benzamidine, and 5 μg/mL pepstatin and leupeptin. After centrifugation at 108,000g for 1 h, the pellet was suspended in 0.5 mL of the same buffer solution and stored at −80°C. The protein concentration was determined with the Bio-Rad reagent (Bio-Rad, Hercules, CA).

Ca2+ Transport

45Ca2+ uptake into vesicles was measured by the filtration method (Hwang et al., 1997). All experiments were conducted with membranes isolated from two to three independent transformants. A reaction mixture (final volume 0.25 mL) contained 10 to 25 μL of vesicles (15–30 μg of protein), 200 mm Suc, 25 mm HEPES-BTP (pH 7.0), 20 mm KCl, 0.1 mm NaN3, 100 μm EGTA, 10 μm 45CaCl2 (1 μCi/mL), and 3 mm MgCl2. Usually 1 μmbafilomycin A and 5 μm carbonyl cyanidem-chlorophenyl hydrazone (CCCP) were included to eliminate activity of the H+/Ca2+exchanger. Transport was usually initiated with 3 mm ATP at room temperature (22°C) for various times. Aliquots (0.23 mL) from duplicate reactions were filtered and washed with 2 mL of cold rinse solution (250 mm Suc, 2.5 mm HEPES-BTP at pH 7.0, and 0.2 mm CaCl2).45Ca2+ retained on the filter was quantitated by liquid scintillation counting. Pump activity can be determined as ATP-dependent transport that is insensitive to bafilomycin and CCCP (Liang and Sze, 1998). However, considerable45Ca2+ binds to membranes in the presence of calmodulin, so pump activity was determined as vanadate-inhibited uptake in the presence of bafilomycin and CCCP. The Na vanadate concentration used was 100 μm. The kinetic parameters were determined using either a Hanes-Woolf or a Lineweaver-Burk plot. The stock concentration of calmodulin was verified spectrophotometrically using an extinction coefficient ε278 of 1,620m −1 cm−1(Yazawa et al., 1990).

Peptide Synthesis and Purification

Peptides corresponding to sequences at the N terminus of ACA2 were synthesized by Research Genetics (Huntsville, AL) via Fmoc solid phase synthesis (Wellings and Atherton, 1997) on chlorotrityl resins with a peptide synthesizer (Advanced ChemTech, Louisville, KY). Peptides were purified by preparative HPLC using a gradient of 0% to 80% (v/v) acetonitrile in 0.1% (v/v) trifluoroacetic acid. All of the fractions were lyophilized and verified by MALDI-TOF using Voyager mass spectrometry. The purified peptides were obtained as a triflouroacetic acid salt. The amino acid composition of the peptide was analyzed with a fully-automated amino acid hydrolyzer/derivatizer (model 420A, ABI, Sunnyvale, CA) coupled with an on-line microbore HPLC analyzer (model 130A, ABI). The internal standard used was α-amino butyric acid. The relative purity of peptide 12-36, 20-44, and 30-54 were 93%, 90%, and 94%, respectively. Peptide stocks of 1 to 2 mm were dissolved in water or in dimethyl sulfoxide, and stored at −20 C.

Sources of Chemicals

Erythrosin B (E-7505), cyclopiazonic acid (C-1530), and bovine brain calmodulin (P-2277) were obtained from Sigma-Aldrich (St. Louis). Thapsigargin was purchased from LC Service (Woburn, MA). All other chemicals were reagent grade.

RESULTS

A Truncated ACA2 Ca2+-ATPase Increased Yeast Mutant Growth on Ca2+-Depleted Medium

The K616 yeast mutant defective in both a Golgi and a vacuolar Ca2+ pump was unable to grow on Ca2+-depleted medium (Harper et al., 1998), although the Ca2+ concentration limiting growth was not determined. We tested growth rates of mutants expressing either a full-length ACA2-1 or a truncated ACA2-2 as a function of Ca2+ concentration. The initial free Ca2+ concentration in the medium was adjusted by adding varying amounts of EGTA to SC-URA medium containing 1 mm Ca2+, and was estimated according to the Max-Chelator program (Bers et al., 1994). In medium containing approximately 50 μm Ca2+, ACA2-1 transformants grew nearly as well as wild-type strains (Fig.1B). However, in medium containing 0.1 to 1 μm Ca2+, mutants expressing the full-length ACA2-1 grew very slowly, like control mutants transformed with vector alone (Fig. 1). Only mutants expressing the N-terminal-truncated protein (Δ2–80) showed growth rates comparable to the wild-type strain (Fig. 1). Thus, the truncated ACA2 protein behaved like an active Ca2+-pumping ATPase on the endomembrane, while the full-length protein appeared to be inactive.

Fig. 1.
Fig. 1.

Expression of an N-terminal truncated ACA2 enhanced growth of yeast mutant K616 on medium containing submicromolar Ca2+. A, Time-course of yeast growth on medium containing 0.16 μm Ca2+ and 10 mm EGTA. K616 strains were transformed with control vector alone (▴), vector containing ACA2-1 (○), and ACA2-2 (●). Transformants were suspended in SC-URA medium containing 10 mm EGTA to an initialA 600 of 0.1. B, Submicromolar levels of extracellular Ca2+ support growth of wild-type yeast and transformants harboring the truncated ACA2-2. Transformants were suspended in SC-URA medium containing varying levels EGTA to give free [Ca2+] ranging from 0.16 to 55 μm. Cells were then incubated for 24 h. Wild-type yeast transformed with vector control (Δ), and K616 mutant transformed with vector alone (▴), ACA2-1 (○), or ACA2-2 (●).

N-Terminal Truncated ACA2 Pump Is More Active Than the Full-Length Protein

To test for Ca2+ pump activity in vitro, yeast transformants were cultured in synthetic medium containing 1 mm Ca2+, and microsomal vesicles were isolated. ATP-dependent45Ca2+ transport was measured by a filtration assay. Both a Golgi (Pmr1p) and a vacuolar (Pmc1p) Ca2+ pump are defective in theK616 strain, so virtually all endogenous Ca2+-pumping activity was eliminated (Liang and Sze, 1998). Most of the ATP-dependent Ca2+ uptake in control transformants was reduced by the protonophore CCCP and a vacuolar H+-ATPase inhibitor, bafilomycin A (Table I), indicating that endogenous H+/Ca2+ antiport activity was high. In membranes from ACA2 transformants, most (>90%) of the transport activity was due to the expressed ACA2, since the transport activity was independent of a pH gradient and sensitive to vanadate.

View this table:
Table I.

Ca2+ pump is detected after blocking H+/Ca2+ antiport activity

It was interesting that the expression of a functional ACA2 pump resulted in suppression of H+:Ca2+ antiport activity in the K616 mutant, although the mechanism for this down-regulation is not known. Therefore, Ca2+-pumping activity was routinely assayed with both CCCP and bafilomycin. Ca2+ uptake by the full-length and truncated pump was enhanced 2- and 6-fold by 10 mm K-oxalate (Table I). Although oxalate amplified the signal, it was not used in subsequent assays to better control free Ca2+ concentration. The expression levels of ACA2-1 and ACA2-2 in isolated yeast membranes were similar, judging by the relative immunostaining of a 110- and a 102-kD protein with a polyclonal antibody against ACA2 (Harper et al., 1998; I. Hwang, data not shown). Therefore, transport activities of the full-length and truncated ACA2 are directly comparable when expressed as nanomoles of Ca2+ taken up per milligram of total membrane protein. Using this analysis, the full-length pump was 10-fold less active than the truncated ACA2 (TableI) on a per milligram of protein basis when net uptake was assayed with 50 nm Ca2+.

45Ca2+ uptake by ACA2 into membrane vesicles was linear for about 30 s and reached a steady state in 5 min in the absence of oxalate (Fig.2). The transport reflects active accumulation, as most of this Ca2+ (>90%) was readily released by the Ca2+ ionophore A23187. The initial rate of Ca2+ pumping (>1 nmol min−1 mg−1 protein) by the truncated ACA2-2 was fast, resulting in a 4- to 10-fold higher net uptake than that of the full-length protein. The control mutants showed low uptake, probably from residual antiport activity that was not eliminated by bafilomycin and CCCP (Table I); therefore, there was little or no background pump activity in the control mutant. Because removal of the first 80 residues generated a more active pump, the results suggested that the amino-terminal region was inhibiting activity.

Fig. 2.
Fig. 2.

N-terminal-truncated ACA2 is an active Ca2+ pump. Membranes were isolated from mutants transformed with ACA2-1 (○), ACA2-2 (●), or vector alone (▴). 45Ca2+ uptake into vesicles was determined in the presence of bafilomycin and CCCP with or without ATP. EGTA was used to give a free [Ca2+] of 0.1 μm. Activity is expressed as ATP-dependent Ca2+ uptake. A23187 was added (arrow) to a final concentration of 2.5 μg/mL.

Calmodulin Enhanced the Vmax of the Full-Length ACA2 Pump

Calmodulin activated Ca2+ pumping of the full-length ACA2 protein in a concentration-dependent fashion, with a half-maximal activation of 30 to 40 nm (Fig.3). However, it had little or no effect on the truncated ACA2-2 pump. In the absence of calmodulin, the specific activity of the truncated ACA2 was 4- to 6-fold higher (6 nmol/mg) than that of the full-length protein (1 nmol/mg). Calmodulin stimulated ACA2-1 activity by 2- to 4-fold, suggesting a direct interaction of calmodulin with the full-length ACA2-1 protein. It is possible that calmodulin activates the pump in more than one way, because activity was sometimes further stimulated at a concentration above 0.3 μm.

Fig. 3.
Fig. 3.

Calmodulin stimulated the full-length ACA2 pump but not the N-terminal truncated ACA2. Membranes were isolated from mutants transformed with ACA2-1 (○),ACA2-2 (●), or vector alone (▴). Net Ca2+ uptake (10 min) into vesicles was measured with or without 200 μm Na orthovanadate in the presence of CCCP and bafilomycin. The mixture contained 100 μmCa2+ and 100 μm EGTA to give a final [Ca2+] of 2.6 μm, and bovine calmodulin ranged from 0 to 1 μm. Activity is expressed as vanadate-sensitive Ca2+ transport. The average of two independent experiments representative of five experiments is shown.

In one representative experiment of four, the full-length ACA2 pump showed an apparent K m for Ca2+ of 0.67 μm, and aV max of 1.67 nmol mg−1min−1 (Fig. 4). Calmodulin (0.5 μm) enhanced the maximal transport rate by 2- to 4-fold. Although the increase in affinity for Ca2+ was small, calmodulin consistently shifted the apparent K m for Ca2+ from 0.62 to 0.43 μm(Table II). We assumed that 1 μm calmodulin does not significantly alter the free Ca2+ concentration that is buffered with 100 μm of EGTA. Thus, the actualK m for Ca2+ in the presence of calmodulin might be even lower than our minimum estimate. The truncated ACA2 showed a high affinity for Ca2+ (K m = 0.25 μm) and a maximal velocity of 5 to 7 nmol mg−1 min−1 (Fig.5; Table II). Although calmodulin stimulated the full-length pump, it usually failed to stimulate it to the levels seen for the truncated protein.

Fig. 4.
Fig. 4.

Calmodulin enhanced theV max and the affinity for Ca2+of the full-length ACA2 protein. Membranes were isolated fromACA2-1 transformants. Initial rate of vanadate-sensitive Ca2+ transport was determined at 30 s with (●) or without (○) 0.5 μm calmodulin in the presence of bafilomycin and CCCP. EGTA (100 μm) was added to reaction mixtures containing 1-100 μm Ca2+ to give free Ca2+ concentrations from 1 nm to 2.6 μm. The K m for Ca2+ was 0.67 or 0.39 μm without or with calmodulin, respectively. The average of duplicates from one of three similar experiments is shown.

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Table II.

Effect of calmodulin or peptide 20–44 on the Vmax and Km for Ca2+ of ACA2

Fig. 5.
Fig. 5.

N-terminal truncated ACA2 pumps Ca2+with high velocity and affinity. Membranes were isolated from yeast expressing ACA2-2. The initial rate of vanadate-sensitive Ca2+ uptake (30 s) was determined as a function of external [Ca2+] in the presence of bafilomycin and CCCP. TheK m for Ca2+ of 0.2 μm was estimated using a Lineweaver-Burk plot. The average of duplicates from one of three experiments is shown.

Substrate Specificity

ATP is the preferred substrate of ACA2. The relative transport activity driven by 3 mm of ATP, ITP, or GTP was 1.0, 0.59, or 0.23, respectively. These results agree with previous studies showing that calmodulin-stimulated Ca2+-pumping ATPases from plants utilized both ITP and GTP (Rasi-Caldogno et al., 1995; Hwang et al., 1997). The ATP concentration dependence of Ca2+ uptake by ACA2 was complex (data not shown). The initial rate of Ca2+ uptake showed two apparent affinities for ATP. The K m of the high-affinity and low-affinity sites were estimated as 120 μm and 1.2 mm, respectively. GTP-driven transport showed one apparent affinity for GTP estimated as being higher than 1.2 mm.

The ACA2 Pump Is Not Inhibited by Cyclopiazonic Acid

Orthovanadate inhibited pumping with an IC50of 30 μm (Fig. 6A), which is consistent with idea that ACA2 is a phosphorylated-type ion-pumping ATPase. Erythrosin B, a potent inhibitor of both PM-type and ER-type Ca2+-ATPase, completely inhibited Ca2+ transport activity of ACA2 at 10 μm (50% inhibition at 0.3 μm) (Fig. 6B). Thapsigargin, a specific animal sarcoplasmic/endoplasmic reticulum Ca2+ (SERCA) inhibitor at 4 nm, had no effect on Ca2+ pumping by ACA2 up to concentrations of 1,000 pmol/mg protein or 100 nm (Fig.6C). In contrast to an Arabidopsis ER-type Ca2+pump (AtECA1), cyclopiazonic acid had no effect on ACA2-catalyzed Ca2+-pumping activity (Fig 6D). This inhibitor blocked the AtECA1 pump by 50% at 3 nmol/mg protein (Liang and Sze, 1998). Inhibition at high concentrations (30–100 nmol/mg protein) is thought to be non-specific, as cyclopiazonic acid completely inhibits the SER Ca2+ pump at 8 nmol/mg protein at low ATP concentrations (Seidler et al., 1989). These results demonstrate that the ACA2 pump is biochemically distinct from the SER-type Ca2+-ATPase consistent with their low sequence similarity (<32%) (Harper et al., 1998).

Fig. 6.
Fig. 6.

Sensitivity of the truncated ACA2 pump to various inhibitors: A, Orthovanadate; B, erythrosin B; C, thapsigargin; and D, cyclopiazonic acid. Vesicles isolated from ACA2-2 transformants were incubated with inhibitors for 10 min at room temperature in the reaction mixtures. Then net ATP-dependent Ca2+ uptake was measured in the presence of bafilomycin and CCCP. Activity (5.2 nmol mg−1 protein−1) without the inhibitors used in A through C was set to 100%. To test cyclopiazonic acid in D, ATP was lowered to 0.6 mm, which gave a control activity of 1.5 nmol/mg.

Truncated ACA2 Pump Is Inhibited by a Peptide That Consists of Residues 20 to 44

To determine the precise region responsible for inhibition, we tested whether synthetic peptides corresponding to overlapping regions in the N-terminal domain could block transport of the constitutively active truncated pump. A 25-residue peptide including Val-20 to Leu-44 inhibited Ca2+ transport of the truncated ACA2 by 50% at 4 μm (Fig. 7 A). In contrast, another peptide that included Lys-12 to Arg-36 had no effect up to 20 μm. A third peptide corresponding to Val-30 to Arg-54 inhibited activity only by 20% inhibition at 20 μm. Since peptide 20-44 consisted of seven positively charged residues at neutral pH, we tested whether a positively charged peptide (+5) conferred inhibition. The CCa20 peptide, with 20 residues, corresponds to the region GR+CTSGAR+SR+SK+SSLK+HK+AE−at the carboxyl terminus of an ER-type Ca2+ pump from carrot (Liang, 1998). However, this peptide had no effect up to 10 μm (not shown). Therefore, the sequence and structure of the 20-44 peptide was sufficient to block the truncated ACA2 pump.

Fig. 7.
Fig. 7.

Peptide 20-44 inhibited Ca2+ pumping of the truncated ACA2 (left panel) and ECA1 (right panel). Membranes isolated from either ACA2-2 or ECA1transformants were incubated with varying concentrations of peptides 12-36 (▴), 20-44 (●), or 30-54 (○). Peptides correspond to overlapping sequences between residues 12 through 54 of ACA2 shown at the top. Charged residues are marked with + or −; asterisk (*) indicates potential phosphorylation sites. Vanadate-inhibited Ca2+ transport (10 min) was measured at 50 nmCa2+. The results are the mean of duplicates. Activity in the absence of peptide was set to 100%, which corresponds to 1.25 and 0.89 nmol/mg for ACA2 and ECA1, respectively.

We tested whether 4 μm of peptide 20-44 changed the kinetic properties of the truncated pump. The affinity for Ca2+ was decreased by 2-fold, so that theK m of the truncated pump increased from 0.25 to 0.51 μm Ca2+(Table II). The maximal transport velocity decreased from 7.07 to 3.34 nmol mg−1 min−1, which is consistent with the inhibition by 50% at 4 μmpeptide (Fig. 7 A). Thus, the peptide altered the kinetic properties of the truncated pump in a manner analogous to that imposed by the intact N-terminal domain on the full-length pump.

Peptide 20-44 Reduces Calmodulin-Stimulated Activity of the Full-Length ACA2

We tested whether peptide 20-44 inhibited the full-length pump in the presence of calmodulin. Membranes were incubated for 10 min with calmodulin in the presence of varying concentrations of peptide before Ca2+ pump activity was determined. Surprisingly, a peptide concentration of 0.6 μm was sufficient to block 50% of the activity stimulated by 1 μm calmodulin (Fig.8). This peptide concentration is significantly (7-fold) lower than that (4 μm) required to block the unregulated truncated pump by 50% (Fig. 7 ). The results suggested that two different mechanisms were responsible for peptide inhibition of the truncated ACA2-2 versus the calmodulin-stimulated full-length ACA2-1.

Fig. 8.
Fig. 8.

Peptide 20-44 antagonized the stimulation of the full-length pump by calmodulin. Membranes isolated fromACA2-1 transformants were incubated with 0, 0.25, or 1.0 μm calmodulin alone, or with peptide 20-44 for 10 min. ATP was added to initiate transport at saturating Ca2+ (2.6 μm). Orthovanadate-inhibited uptake at 10 min is plotted. In the absence of peptide, the activity (100%) stimulated by 0.25 or 1.0 μm calmodulin was 2.85 or 4.0 nmol/mg, respectively. The data are from two experiments.

Furthermore, the peptide concentration required for half-maximal inhibition of the full-length pump was dependent on the calmodulin concentration. At 0.25 μmcalmodulin, 0.15 μm peptide was required to inhibit activity by 50% (Fig. 8), whereas at 1 μm calmodulin, 0.6 μm peptide blocked 50% of the activity. Thus, the peptide antagonizes the stimulation of the full-length pump by calmodulin in a concentration-dependent manner. Because calmodulin was previously shown to bind to the N-terminal region within residues 20 to 36 (Harper et al., 1998), one interpretation of these results is that peptide 20-44 interacts directly with calmodulin. If this is the case, then peptide 20-44 inhibits the calmodulin-activated full-length Ca2+ pump indirectly according to the following model: Peptide 20-44 competes with the corresponding region in the intact N-terminal domain of the pump for calmodulin binding. When the peptide level is low, calmodulin interacts with the N-terminal region to activate the pump. With excess peptide, most of the calmodulin interacts with the peptide, leaving little or no calmodulin to activate the full-length pump. Therefore, most of the pumps remain in their autoinhibited state.

Peptide 20-44 Also Inhibits Ca2+ Pumping by ECA1

Peptide 20-44 was also effective in blocking transport of an ER-type Ca2+ pump, ECA1, from Arabidopsis (Liang et al., 1997; Liang and Sze, 1998). The concentration required for 50% inhibition was about 6 to 7 μm, and 70% of the activity was blocked by 12 μm (Fig. 7B). Therefore, 20 -44 was nearly as potent in blocking ECA1 as the ACA2 pump, suggesting that the target site of the peptide may be conserved among two distinct Ca2+ pumps.

DISCUSSION

An important step in understanding the regulation of intracellular Ca2+ in plants is to determine the kinetic properties of each Ca2+ transporter and the conditions that modulate its activity. Arabidopsis has at least 11 putative Ca2+ pumps (Geisler et al., 1999), so the distinct properties, regulation, and location of each are important in determining the pattern or form of Ca2+ signals within the cell. AtECA1encodes the first plant Ca2+ pump to be characterized biochemically after expression in yeast (Liang and Sze, 1998). However, it is not known how the ER-localized ECA1 pump is regulated in plants. We provide the first direct evidence, to our knowledge, that ACA2 encodes another Ca2+ pump, and show that its kinetic properties are modulated by calmodulin and by an autoinhibitory sequence.

The Amino-Terminal Domain Contains an Autoinhibitor

We determined the kinetic properties of the truncated and the full-length pump, and show that in the absence of Ca2+/calmodulin, the N-terminal region (between residues 2–80) functions as an autoinhibitor that decreases theV max by 3-fold and the affinity for Ca2+ by 2.5-fold (Table II). The quantitative differences are probably underestimated, as the transport reported in Table II was conducted in the absence of oxalate. In its presence, Ca2+ uptake is prolonged, as Ca2+ in the vesicles is trapped by oxalate and back inhibition is prevented. Under these conditions, the N-terminal-truncated ACA2-2 pump was 10-fold more active than the full-length ACA2-1 protein (Table I). These results are consistent with an initial qualitative study that used ATP hydrolysis to measure the activity of a full-length and a truncated Ca2+-ATPase (Harper et al., 1998). Assuming that the relative activities in vitro are comparable to those in yeast transformants, these results suggest that the basal activity of the full-length protein was not sufficient to pump enough Ca2+ into the ER/Golgi to support growth on Ca2+-depleted medium (containing 0.1–1.0 μm Ca2+).

Sequence Including Residues 20 to 44 Behaves Like the Autoinhibitory Domain

Our results indicate that a 25-residue sequence from Val-20 to Leu-44 behaved like the inhibitory region. This conclusion is supported by the following results: (a) peptide 20-44 blocked 50% activity at a concentration of 4 μm (Fig. 7A); (b) two other peptides, Lys-12-Arg-36 and Val-30-Arg-54, of similar size, charge, and partial overlapping sequence had little or no effect; and (c) peptide 20-44 at 4 μm increased the K mfor Ca2+ and decreased theV max of the truncated pump to levels approaching the basal activity of the full-length pump (Table II). Thus, the peptide mimicked the natural inhibitor of the pump. The micromolar levels of peptide required for inhibition was similar to other chemical inhibitors, which must “find” its target site on ACA2 in a dilute membrane mixture. Our results suggest that the secondary and tertiary structures of peptide 20-44 are sufficient for inhibition.

Calmodulin Partially Activates the Full-Length Pump

In a previous study, we showed that ATP hydrolysis by the full-length protein was increased by 100 μmCa2+, and that this Ca2+-ATPase activity was further stimulated by calmodulin (Harper et al., 1998). However, the affinity of ACA2 for Ca2+ was not known. Here we demonstrate that calmodulin stimulated the V max and increased the affinity of the full-length ACA2-1 for Ca2+ (Table II). Furthermore, the affinity for Ca2+ of the calmodulin-stimulated full-length pump (K m = 0.4 μm) and of the truncated pump (K m = 0.2 μm) was about 2- and 3-fold higher, respectively, than that of the basal ACA2-1 (K m = 0.64 μm) (Table II). Thus, ACA2 is active in the physiological range of Ca2+ fluctuations within the cytosol (0.1–1.0 μm). Assuming that theV max of the truncated pump represents the latent potential of a full-length pump, only a partial activation of the full-length pump occurs with the addition of calmodulin. It is also possible that other modulators, such as phosphorylation or lipids, could further shift the steady-state balance between an activated and autoinhibited state.

Calmodulin Interacts with the Autoinhibitory Region

Interestingly, results from two separate approaches support the idea that the calmodulin-binding region overlaps with the inhibitory region. First, a calmodulin-binding site was identified between residues 20 and 36, as shown by the Ca2+-dependent binding of calmodulin to a series of fusion proteins from the N-terminal domain (Harper et al., 1998). Second, peptide 20-44 blocked the calmodulin activation of a full-length pump (Fig. 8). This peptide inhibition was prevented by increasing the concentration of calmodulin, which is consistent with a model that the peptide competes with the pump for binding calmodulin. As the peptide concentration increases, less free calmodulin is available for interaction with the full-length pump, and thus most of the pumps remain in an inhibited conformation. The peptide at 0.2 and 0.8 μm inhibited nearly 80% of the pump activity stimulated by 0.25 and 1 μm calmodulin, respectively, supporting a model that the peptide binds calmodulin with high affinity.

Although other models are possible, the inhibition of calmodulin-stimulated ACA2 by peptide 20-44 is strikingly similar to the effect of an autoinhibitor peptide on a calmodulin-stimulated plasma membrane Ca2+-ATPase from human erythrocytes (Enyedi et al., 1989; Penniston and Enyedi, 1998). The concentration of peptide C28W required for half-maximal inhibition was highly dependent on the calmodulin concentration. Furthermore, in the presence of Ca2+, peptide C28W interacted tightly with calmodulin, forming a 1:1 complex. Our results support the idea that calmodulin binds to a region overlapping the autoinhibitory domain within residues 20 to 44. The degree of overlap between the two regulatory domains has yet to be determined, since a complete calmodulin-binding domain possibly extends beyond residue 36.

Role of ACA2 and a Model for Regulation

ACA2 was recently localized to the ER of Arabidopsis roots by membrane fractionation, immunostaining, and imaging of a green fluorescent protein-tagged enzyme (Hong et al., 1999). The presence of calmodulin-stimulated Ca2+ pumps at the ER was regarded with skepticism for many years, because calmodulin-insensitive Ca2+ pumps such as AtECA1 are commonly associated with the ER (Liang et al., 1997). However, biochemical studies using a variety of plant tissues had initially suggested that ER was another site for calmodulin-stimulated Ca2+ pumps (Hwang et al., 1997, and refs. therein). Although it is unclear what roles two differentially regulated Ca2+ pumps play at the ER (Hong et al., 1999), the results support the idea for discrete functional domains (Staehelin, 1997) and for spatially and functionally distinct Ca2+ stores (Golovina and Blaustein, 1997) in the ER. Signals that stimulate ER functions, including protein assembly, modification, and vesicle trafficking, could conceivably modulate ER-localized Ca2+ pumps. For instance, GA3-stimulated a 2-fold increase in calmodulin and Ca2+ uptake in the ER of barley aleurone cells, suggesting that elevated calmodulin and Ca2+ in the ER may regulate Ca2+-dependent α-amylase synthesis in the lumen of the ER (Gilroy and Jones, 1993).

We propose a working model for the regulation of the ACA2 pump (Fig.9). In a resting cell, ACA2 is less active due to an interaction of the N-terminal autoinhibitory region including residues 20 to 44 with an intramolecular hydrophilic region. After a signal induces an increase in calmodulin or in cytosolic Ca2+ near ACA2 (or both), the Ca2+-calmodulin complex binds to a region including residues 20 to 36. This binding confers a conformational change to the N-terminal domain, resulting in the dissociation of the inhibitory region from the hydrophilic loop(s) leading to an activated state. The N-terminal truncated protein is therefore constitutively active as the inhibitory domain is absent. However, it can be inactivated by a synthetic peptide with a sequence (residues Val-20 to Leu-44) corresponding to the inhibitory domain.

Fig. 9.
Fig. 9.

Working model of the regulation of ACA2 pump by an N-terminal domain. In an unstimulated cell, the full-length pump (ACA2-1) is inhibited by an intramolecular interaction of an N-terminal region with part(s) of the catalytic and hydrophilic loop(s) (top). After a stimulus, an increase in [Ca2+] leads to the binding of Ca2+-calmodulin complex to a region near the autoinhibitory sequence. The resulting conformational change displaces the autoinhibitory domain from its interaction with the hydrophilic loop(s), thus activating the pump (left). An N-terminal truncated pump (ΔN2-80) is therefore constitutively active and unresponsive to calmodulin (right). Numbers refer to putative transmembrane domains. N and C refer to the amino and carboxyl termini, respectively.

It is unclear what part(s) of the pump interacts with the autoinhibitory sequence within residues 20 to 44 at the N-terminal domain. The structural elements that interact with the peptide may be common in two distinct types of Ca2+ pumps, as peptide 20-44 also blocked Ca2+ pumping of AtECA1 (Fig. 7B). Interestingly, a synthetic peptide having the sequence of the autoinhibitory domain of the animal plasma membrane Ca2+-ATPase also inhibits SERCA activity (Enyedi and Penniston, 1993). Several highly conserved regions common to P-type ATPases include the region surrounding the aspartyl phosphorylation site and that involved in ATP binding between transmembrane regions 4 and 5. One working model is that the N-terminal autoinhibitory domain interacts with sites located within or near two cytoplasmic loops located between TM4 and TM5 and between TM2 and TM3. In cross-linking studies, the peptide from the C-terminal domain of plasma membrane Ca2+-ATPase appears to interact with a region between the acyl phosphorylation site and the ATP-binding site (Falchetto et al., 1991). Thus, in spite of an amino-terminal location in ACA2, the regulatory domains in both animal and plant pumps appear to have evolved with a common mechanism of regulation (James et al., 1995).

The regulation of ACA2 bears striking similarities to a 133-kD plasma membrane Ca2+ pump (Rasi-Caldogno et al., 1995) and a 111-kD vacuolar Ca2+ pump (Askerlund, 1996). In both cases, trypsin treatment of membrane vesicles resulted in a 2- to 3-fold activation of Ca2+ pumping and loss of calmodulin sensitivity. The activation by trypsin was accompanied by a decrease of the intact vacuolar Ca2+ pump of 111 kD, and the appearance of a 102-kD polypeptide. However, only the 111-kD protein bound calmodulin, suggesting that trypsin had removed the calmodulin-binding region. The location of the calmodulin-binding region was unclear until the corresponding gene, BCA1, was cloned. Calmodulin was shown to bind with high affinity (K D = 75 nm) to a peptide corresponding to Ala-19-Leu-43 (Malmstrom et al., 1997). Thus, a vacuolar BCA1, an ER-localized ACA2, and possibly a plasma membrane Ca2+ pump (M.I. de Michelis, personal communication) are all regulated by an N-terminal domain similar to the model of Figure 9. Additional homologs of ACA2 and BCA1 are being identified in Arabidopsis (for review, seeGeisler et al., 1999), so a future challenge is to understand the distinct cellular and physiological roles of each Ca2+ pump in plants.

ACKNOWLEDGMENTS

We thank Rajini Rao (Johns Hopkins University, Baltimore) and John Penniston (Mayo Clinic, St. Paul) for stimulating and enlightening discussions.

Footnotes

  • ↵1 This work was supported in part by the Department of Energy (grant no. DE–FG02–95ER20200 to H.S. and grant no. DE–FG03–94ER20152 to J.F.H.), and a joint grant from the National Aeronautics and Space Administration and National Science Foundation (grant no. IBN–9416038) for the Plant Sensory Systems Collaborative Research Network.

  • ↵2 Present address: Department of Bioinformatics, The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850.

  • ↵* Corresponding author; e-mail hs29{at}umail.umd.edu; fax 301–314–9082.

  • Received July 26, 1999.
  • Accepted September 10, 1999.

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Calmodulin Activation of an Endoplasmic Reticulum-Located Calcium Pump Involves an Interaction with the N-Terminal Autoinhibitory Domain
Ildoo Hwang, Jeffrey F. Harper, Feng Liang, Heven Sze
Plant Physiology Jan 2000, 122 (1) 157-168; DOI: 10.1104/pp.122.1.157
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