Article Figures & Data
Figures
- Fig. 1.
Expression of the FATB thioesterase in different rapeseed tissues. A, Total RNA was isolated from different rapeseed tissues and from developing flowers and 5 μg each was loaded on an RNA gel. After blotting, the RNA was hybridized with the AtFATB1 cDNA. B, Protein (50 μg) was isolated from the same tissues and blotted onto a nitrocellulose membrane. The rapeseed FATB gene product was detected on the blot with anti-AtFATB protein antibodies. The developmental stages of the flower buds were: Bud 1, 1 to 5 mm long; Bud 2, 5 to 8 mm; Bud 3, 8 to 10 mm; and Bud 4, 10 to 12 mm.
- Fig. 2.
Expression of the AtFATB1 thioesterase in different Arabidopsis tissues. A, Total RNA was isolated from different Arabidopsis tissues and 10 μg was loaded in each lane. The northern blot was hybridized with the AtFATB1 cDNA. B, Protein (40 μg) was isolated from developing flowers, green siliques, and green seeds. After blotting to nitrocellulose, the AtFATB1 polypeptide was detected with anti-AtFATB1 protein antibodies. Bud 1, 0 to 1 mm long; Bud 2, >1 mm.
- Fig. 3.
16:0 content in Arabidopsis wild-type andAtFATB1-antisense lines. A, Flowers; B, mature seeds; C, roots; D, leaves; E, phosphatidylethanolamine of leaf tissue isolated and quantified by thin-layer chromatography and gas liquid chromatography of fatty acid methyl esters. The values represent means ± se of three measurements.
Tables
Line 14:0 16:0 18:0 18:1 18:2 18:3 20:1 22:1 mol % Wild type 0.1 10.0 3.1 12.1 32.9 23.5 16.6 1.6 TES130 0.7 38.8 6.4 6.0 27.8 13.5 6.3 0.5 TES182 0.6 26.6 5.2 6.7 31.1 17.6 11.1 1.1 TES184 0.4 33.7 5.5 7.4 24.8 18.3 9.2 0.8 Batches of 20 to 40 seeds of the wild type and of each transgenic line were analyzed.
- Table II.
Fatty acid content of total lipids from different tissues of Arabidopsis wild-type plants
Tissue 14:0 16:0 16:1 16:3 18:0 18:1 18:2 18:3 20:1 22:1 mol % Total flowers 1.1 26.7 0.9 1.9 2.1 2.6 27.7 37.0 n.d. n.d. Green seeds 0.2 10.5 0.8 0.3 4.1 11.5 28.3 25.0 16.8 2.5 Mature seeds 0.2 11.9 0.2 n.d. 3.8 12.7 33.1 20.6 15.6 1.8 Sepals n.d. 26.0 4.2 7.8 1.7 n.d. 17.7 42.6 n.d. n.d. Petals 1.2 28.9 0.8 1.0 2.6 1.4 34.3 29.6 n.d. 0.2 Stamen 2.5 26.8 1.0 0.8 2.7 7.0 21.2 38.0 n.d. n.d. Pistils 0.6 24.4 0.1 1.3 2.3 2.9 33.7 34.6 n.d. 1.1 Silique coats n.d. 19.0 2.3 6.1 3.0 4.0 21.7 43.5 n.d. 0.4 Leaves 0.6 13.6 3.9 13.9 0.8 2.8 11.9 52.5 n.d. n.d. Roots 0.6 22.3 2.2 0.3 2.1 6.7 38.9 26.6 n.d. 0.3 Stems n.d. 19.3 3.0 7.0 1.1 1.0 17.8 50.8 n.d. n.d. The data represent the means of three measurements. The sdwas always below 10% of the given value. n.d., Not detectable.
Lipid Fraction Total Lipids 14:0 16:0 16:1 18:0 18:1 18:2 18:3 20:1 22:1 μmol fatty acid g−1fresh wt mol % Wax 0.77 ± 0.06 15.7 32.3 1.1 4.3 30.5 7.8 6.3 0.3 n.d. TAG 0.30 ± 0.01 5.2 19.4 5.0 2.5 62.3 3.0 0.5 0.7 1.0 Polar lipids 4.20 ± 0.62 0.2 48.6 0.6 2.3 8.6 22.4 15.2 n.d. 0.6 Total lipids represent the means ± sd of two measurements. The fatty acid composition is from two measurements;sd was below 5% for all fatty acid values. n.d., Not detectable.
