Article Figures & Data
Figures
- Fig. 1.
Analysis of HY5 in mediating light activation of its target promoter. A, Rbcs promoter activity as estimated via GUS from RbcS-GUS transgenic seedlings grown in continuous white light for 5 d (top). Seedlings were grown in decreasing light intensities from left to right. The fluence rates were 127.5, 19.0, 8.7, 2.8, and 0.6 μmol s−1m−2, respectively (Osterlund et al., 2000). GUS activity is measured in arbitrary units. αHY5 western on the seedlings used for the GUS assay is shown at the bottom. B, The RbcS-GUS activity from wild type and the hy5–1 seedlings grown in continuous darkness (D), white light (W), red light (R), blue light (B), and far-red light (FR). The reporter GUS activity is measured in arbitrary units. All error bars indicatesd between four samples.
- Fig. 2.
Characterization of the hy5 enhancer mutants. A, Enhancer mutants are shown in comparison with the parentalhy5-ks50 mutant. Seedlings were grown in continuous white light for 5 d. Left, The hy5 seedling beside ahy1/hy5 (88.1) double mutant. Right, Thehy5 seedling beside a hy2/hy5 (134.1) double mutant. B, The representative F2segregating seedlings from the hy2/hy5 double mutant crossed to a wild-type plant. The αHY5 western corresponding to the segregating populations is shown in the bottom. The western verifies the wild-type (WT), hy5, and hy2 genotypes in the four types of segregants. C, Genomic map positions of the sevenhy5 enhancer mutations as determined by PCR-based mapping.
- Fig. 3.
Effect of hy1 mutation on GUS-COP1 nuclear localization. A, Subcellular localization of GUS-COP1 in representative wild-type and hy1 seedlings. a, Typical cytoplasmically localized GUS-COP1 in a hypocotyl cell from wild-type seedlings grown in continuous white light. b, Typical nuclear-localized GUS-COP1 in a hypocotyl cell from wild-type seedlings grown in continuous darkness. c, Nuclear-localized GUS-COP1 in a hypocotyl cell from hy1 seedlings grown in continuous white light. Arrows indicate the locations of the nuclei. B, The subcellular localization of GUS-COP1 in wild-type and hy1 seedlings grown in white light, red light, blue light, and far-red light. Seedlings showing strong nuclear staining of GUS-COP1 received a value of +++, seedlings with slight but obvious nuclear staining received a value of ++, and seedlings displaying no observable nuclear staining received a value of +.
