Article Figures & Data
Figures
- Fig. 1.
The effect of naringenin and NPA on Arabidopsis root development. Photographs showing seedlings grown for 12 d on 100 μm naringenin (A) or 0.1% (v/v) ethanol control (B). C, Roots were grown for 12 d on naringenin and NPA. Gravity response was measured 24 h after reorientation of roots 90 degrees relative to the gravity vector. Values represent the average andse of 10 seedlings per data point.
- Fig. 2.
Comparison of phenotype of WT andtt4(2YY6) plants. A, The aerial phenotype of representative WT (left) and tt4(2YY6) (right) plants were compared 37 d after planting. B, Secondary root development of three WT seedlings (left) and three tt4(2YY6) seedlings (right) grown under continuous light for 13 d are compared.
- Fig. 3.
Quantification of inflorescence phenotypes of WT and tt4 (2YY6) plants. A, Primary inflorescence height was monitored from d 28 until d 55 after planting. B, The number of secondary inflorescences were measured over time.
- Fig. 4.
Comparison of basipetal IAA transport over time in WT and tt4(2YY6) plants. Transport at 2, 6, or 18 h was measured using 28 nm[3H]IAA. Each value represents the average andse of four segments.
- Fig. 5.
Comparison of [3H]IAA transport measurements down the inflorescence stem of WT andtt4(2YY6) plants. Transport was measured on adjacent segments down the stem after 9 h in 134 nm[3H]IAA. Data represent the average andse of five segments. IAA transport in the presence of NPA was similar between WT and tt4(2YY6), averaging 98 and 96 cpm, respectively.
Tables
WT tt4(2YY6)1-a Adult aerial phenotype1-b Primary inflorescence length (cm) 43 ± 1 29 ± 11-165 Number of lateral branches 2.5 ± 0.2 1.8 ± 0.2* Inflorescence stem diameter (mm) 1.1 ± 0.04 0.70 ± 0.021-165 No. of 2° inflorescence stems 4.0 ± 0.3 12.0 ± 0.41-165 Anthocyanins Yes No Root development1-c Adventitious root no. 0.8 ± 0.2 2.2 ± 0.31-165 Lateral root no. 4.5 ± 0.5 8.8 ± 0.91-165 Primary root length (mm) 35 ± 0.8 38 ± 1.1* -
↵F1-a The phenotypic characteristics were compared for WT and tt4(2YY6) using a two-tailed Student'st test and the P values are reported.
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↵* , P < 0.05;
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↵F1-165 , P < 0.001.
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↵F1-b Fifty-five-day-old plants were grown in a 21°C incubator programmed for 16 h of light (90 μmol s−1 m−2) followed by 8 h of dark. Reported values are the average and se of six individual plants per genotype.
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↵F1-c Seven-day-old seedlings were grown under continuous white light (80–90 μmol s−1 m−2) at room temperature. Reported values are the average and se of 10 seedlings per genotype.
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Basipetal IAA Transport2-a Columbia2-b tt4(2YY6)2-b Ler2-c tt4(85)2-c cpm Minus NPA2-d 667 ± 100 1176 ± 114 675 ± 41 1131 ± 41 Plus NPA2-d 15 ± 1 17 ± 1 254 ± 36 255 ± 37 -
↵F2-a IAA transport was measured on basal inflorescence segments after 5 h using 100 nm[3H]-IAA.
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↵F2-b Reported values are average and se of 30 to 40 replicates and transport was measured in the presence and absence of 100 μmNPA.
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↵F2-c Reported values are the average and se of 10 replicates and transport was measured in the presence and absence of 10 μmNPA.
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↵F2-d Comparison of basipetal IAA transport in the absence of NPA indicates that the difference between Columbia and tt4(2YY6) and between Ler and tt4(85) are statistically different with P values < 0.001, as determined by Student's t test analysis. In the presence of NPA there is no significant difference between basipetal transport in Columbia and tt4(2YY6) or Ler and tt4(85) withP values > 0.2, as determined by Student's ttest.
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Basipetal IAA Transport3-a Hypocotyl Root Ler tt4(85) Ler tt4(85) cpm Control medium3-b 517 ± 33 683 ± 38 136 ± 2 274 ± 4 Naringenin 354 ± 44 508 ± 14 66 ± 10 52 ± 6 NPA 354 ± 16 385 ± 23 66 ± 4 89 ± 17 -
↵F3-a IAA transport was measured in hypocotyl and root segments grown on control medium in the presence or absence of 10 nm naringenin or NPA. Reported values are the average and sd of 10 replicates.
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↵F3-b Comparison of basipetal IAA transport in the absence of NPA indicates that the difference between WT Ler and tt4(85) is statistically different with aP < 0.005, as determined by Student's ttest analysis.
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- Table IV.
Comparison of [3H]-NPA binding constants in WT and tt4(2YY6) rosette microsomal membranes
Binding Constant4-a WT tt4(2YY6) B max 4-b 0.15 ± 0.02 0.20 ± 0.04 K d 4-b 15.7 ± 2.2 18.5 ± 4.1 -
↵F4-a Reported values were derived from double-reciprocal plots and represent the average and se of four experiments using six concentrations of [3H]-NPA.K d is calculated as −1/x intercept and is reported in nanomoles; B max is defined as 1/y intercept and is reported as picomoles per milligram.
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↵F4-b Student's t test analysis revealed no significant difference in K d orB max values between WT and tt4(2YY6) microsomal membranes.
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