Article Figures & Data
Figures
- Fig. 1.
Glucosinolate biosynthetic pathway. I, 2-Alkylmalic acid; II, 3-alkylmalic acid; III, 2-oxo acid. A, Basic glucosinolate structure. B, Outline of the pathway which can be divided into three parts: elongation of the amino acid side chain, formation of the basic glucosinolate skeleton, and further side chain modification. Each chain elongation cycle adds an additional methylene group (Graser et al., 2000).
- Fig. 2.
Side chain modifications of Met-derived glucosinolates in Arabidopsis. Potential side chain modifications for the elongated Met derivative, C4 dihomo-Met, are shown. Steps with natural variation identified in this study are shown in bold to the right or left of each enzymatic arrow.
- Fig. 3.
Effect of GS-OX on conversion of methylthioalkyl to methylsulfinylalkyl glucosinolates. Bar diagrams show the ratio of methylthioalkyl (MT) glucosinolate to methylsulfinylalkyl (MSO) glucosinolate content in leaf (A) and seeds (B) for each ecotype analyzed.
- Fig. 4.
GS-AOP regulates the accumulation of aliphatic glucosinolates in the leaves of Arabidopsis. The bars depict the average total aliphatic glucosinolate accumulation in leaves of 39 ecotypes. The ecotypes are classified based on the inferred genotype at three biosynthetic loci: GS-Elong, either C3- or C4-accumulating ecotypes; GS-AOP, alkenyl-, hydroxypropyl-, or methylsulfinylalkyl-containing ecotypes; and GS-OH, functional or nonfunctional alleles.
- Fig. 5.
Correlation of glucosinolate accumulation between the leaves and seeds. A, Scatter plot depicting the relationship between aliphatic glucosinolate accumulation in leaves (ALIPHL) and seeds (ALIPHS) of the ecotypes tested. The 90% confidence ellipse interval is drawn for reference. The values are in μmol g dry weight−1. B, Scatter plot depicting the relationship between indolic glucosinolate accumulation in the leaves (INDOLEL) and seeds (INDOLES) of the ecotypes tested. The values are in μmol g dry weight−1.
- Fig. 6.
Map of GS-OX on Chromosome I. Ninety-two F2 progeny were scored for the microsatellites indicated and for the GS-OX biochemical phenotype. The distance from the AthGeneA and nga692 markers to GS-OX is shown in cM to the right of the arrows. The approximate location of GS-OX is shown to the left of the chromosome.
- Fig. 7.
Correlation of C7 to C8 and C3 to C4 intermediates in the biosynthesis of chain-elongated Met-derived glucosinolates. A, Scatter plot showing the correlation of the conversion of C3 to C4 in the seeds and leaves. B, Scatter plot showing the correlation of the conversion of C7to C8 in the seeds and leaves. C, Scatter plot showing the correlation of the conversion of C3to C4 with C7 to C8 in the leaves. D, Scatter plot showing the correlation of the conversion of C3 to C4 with C7 to C8 in the seeds.
Tables
Ecotype Genotype 1 2 3 4 5 6 7 8 11 E A OH OX Ag-0 4 3 3 2 – – – 1.55 – 4.69 1.56 8.10 Bla-10 4 3 3 1 – – – 0.40 0.83 1.54 0.19 0.36 2.19 Bs1 4 3 3 2 – – – 1.43 – 4.29 1.24 5.65 Cal 4 3 3 2 – – – 0.32 – 1.01 4.90 11.99 Cnt 4 3 3 2 – – 0.31 2.47 – 7.17 0.28 12.46 Ema-1 4 3 3 2 – – 0.13 1.93 – 5.83 0.21 8.28 Pog-0 4 3 3 2 0.06 – 0.32 2.73 – 7.98 0.21 9.39 Tac 4 3 3 2 – 0.05 0.15 2.27 – 6.77 0.35 7.38 Kas 4 3 2 2 – – – – – – 5.31 11.64 Sorbo 4 3 2 2 – – – – – – 4.55 20.96 Cvi 4 3 1 2 – – – – – – 10.03 24.40 Di-1 4 2 – 2 1.79 0.03 – – 3.62 – – – Aa-0 4 1 – 2 – – – – 1.31 – 0.07 0.45 Col 4 1 – 2 – 0.37 – – 4.96 – 0.03 – Ma-0 4 1 – 2 – – – – 2.39 – – – Mt-0 4 1 – 2 – – – – 3.98 – – – Can 3 3 – 1 – 0.61 – – – – 18.43 0.40 Kondara 3 3 – 2 0.11 – – – – – 25.99 0.47 Ei-2 3 3 – 2 – – – – – 0.04 16.79 0.19 Hodja 3 3 – 2 – – – – – – 13.10 0.18 Ita-0 3 3 – 2 – – – – – – – – Kil-0 3 3 – 2 – – – – – – 14.57 0.19 Mr-0 3 3 – 2 – – – – – – – – Mrk-0 3 3 – 2 0.15 – – – – – 20.27 0.29 Rsch-0 3 3 – 2 – – – – – – 10.02 0.22 Su-0 3 3 – 1 – 0.26 – – – – 12.01 0.07 Wl-0 3 3 – 2 0.06 – – – – – 14.38 0.16 Bl-1 3 2 – 2 7.32 – – – – – 0.20 0.03 Di-g 3 2 – 2 – – – – – – – – Ka-0 3 2 – 2 14.32 0.04 – – – – – – Ler 3 2 – 2 9.07 0.17 – – 0.02 – – – Lip-0 3 2 – 2 5.14 0.68 – – – – – – No-0 3 2 – 2 7.22 0.34 – – 0.08 – – – Pet 3 2 – 2 8.55 0.69 – – 0.03 – – – Pi-0 3 2 – 2 2.07 – – – 0.08 – – – Sei-0 3 2 – 2 3.76 0.99 – – 0.07 – – – Tsu-1 3 2 – 2 5.19 0.62 – – 0.05 – – 0.02 Yo-0 3 2 – 2 5.40 0.49 – – 0.20 – – – Oy-0 3 1 – 2 – – – – – – – – Average – – – – 4.68 0.41 0.23 1.64 1.36 4.37 7.60 5.44 sd – – – – 4.15 0.30 0.10 0.90 1.78 2.89 7.96 7.08 Minimum – – – – 0.06 0.03 0.13 0.32 0.02 0.04 0.03 0.02 Maximum – – – – 14.32 0.99 0.32 2.73 4.96 7.98 25.99 24.40 13 14 15 16 18 19 20 21 22 25 26 30 32 – – 0.04 0.21 0.46 – 1.11 3.19 0.36 0.02 0.57 0.10 0.71 – 0.84 0.02 0.17 0.05 4.95 1.12 1.70 0.42 – 0.43 0.10 0.41 0.02 – 0.04 0.22 0.36 – 1.07 2.53 0.31 – 0.21 0.12 0.36 0.09 0.16 0.03 0.36 0.51 – 1.77 2.43 0.47 0.09 0.28 0.21 0.52 0.12 – 0.03 0.42 0.83 0.05 3.00 3.14 0.32 0.01 0.39 0.09 0.56 0.10 – 0.01 0.34 0.60 – 1.84 3.24 0.20 0.01 0.33 0.11 0.42 0.11 – 0.03 0.37 0.73 – 1.87 2.98 0.31 0.03 0.76 0.06 0.36 0.05 – 0.01 0.25 0.47 0.04 1.41 3.16 0.09 0.01 0.33 0.06 0.26 – – 0.02 0.06 0.09 0.02 0.49 1.49 0.35 0.02 0.96 0.04 0.29 – – 0.02 0.17 0.30 – 1.21 1.73 0.31 0.02 0.45 0.07 0.29 0.09 – 0.01 0.29 0.29 – 2.24 1.08 0.19 – 0.39 0.09 0.38 0.01 – 0.04 0.24 – 0.51 1.24 2.82 0.44 0.03 0.56 0.05 0.20 – – 0.02 0.11 – 0.53 1.08 1.87 0.28 – 0.04 – 0.21 – – 0.02 0.16 – 0.97 0.99 2.88 0.31 – 0.62 0.03 0.19 – – 0.02 0.10 – 0.15 0.86 1.73 0.32 – 0.52 – 0.07 – – 0.05 0.15 – 0.30 1.13 2.81 0.52 – 0.78 – 0.15 – 3.66 0.01 0.18 – 0.27 1.77 2.14 0.47 – 0.38 0.15 1.00 – 0.04 – 0.11 – – 1.89 2.62 0.42 – 1.01 0.03 0.53 – – 0.01 0.12 – – 1.76 2.33 0.53 – 0.48 0.08 0.86 – – – – – – 0.61 1.16 0.16 – 0.49 0.03 0.30 – – – – – – – – – – – – – – 0.06 0.01 0.14 – – 1.82 2.52 0.25 – 0.34 0.08 0.58 – – – – – – – – – – – – – – – 0.01 0.12 – – 2.12 2.09 0.16 – 1.07 0.06 0.56 – – – 0.09 – – 1.34 1.88 0.19 – 0.71 0.01 0.24 – 2.02 0.01 0.07 – 0.06 1.36 1.97 0.25 – 0.64 0.06 0.79 0.02 0.01 0.01 0.15 0.05 – 1.22 2.00 0.18 – 0.78 0.02 0.18 – 0.08 0.02 0.10 – 0.05 1.68 1.76 0.33 – 0.94 0.03 0.67 – – – – – – – – – – – – – – – 0.01 0.08 – – 1.54 1.78 0.26 – 0.90 0.04 0.45 – – 0.02 0.07 – – 1.32 1.94 0.21 – 0.71 0.03 0.33 – 0.04 0.01 0.08 – – 1.04 1.73 0.33 – 0.76 0.02 0.27 – 0.09 0.03 0.03 – 0.03 0.88 1.77 0.18 – 0.32 0.04 0.52 – 0.14 0.03 0.08 – 0.06 1.69 2.82 0.26 – 1.13 0.04 0.50 – – – 0.04 – – 0.64 0.93 0.05 – 0.46 – – – 0.02 0.05 0.10 – – 1.11 2.06 0.06 – 0.38 – 0.07 – 0.21 0.03 0.09 – 0.04 1.13 2.77 0.15 – 0.51 0.05 0.40 – 0.12 0.02 0.07 – 0.01 0.81 2.91 0.11 – 0.77 0.04 0.35 – – – – – – – – – – – – – 0.07 0.54 0.02 0.16 0.40 0.50 1.38 2.23 0.28 0.03 0.58 0.06 0.41 0.04 1.05 0.01 0.10 0.26 1.22 0.52 0.63 0.13 0.03 0.26 0.04 0.22 0.01 0.01 0.01 0.03 0.05 0.01 0.49 0.93 0.05 0.01 0.04 0.01 0.07 0.12 3.66 0.05 0.42 0.83 4.95 3.00 3.24 0.53 0.09 1.13 0.21 1.00 Totals Aliph Indole MT MSO AOP C3 C4 C7 C8 C4Per C8Per 18.49 4.16 0.81 1.32 16.36 1.56 14.34 0.31 1.82 0.902 0.854 13.15 2.57 6.30 2.12 4.37 1.03 9.91 0.27 1.53 0.906 0.850 14.76 3.09 0.48 1.31 12.97 1.24 11.37 0.34 1.43 0.902 0.808 21.84 3.21 0.89 2.22 18.73 5.06 13.32 0.57 2.29 0.725 0.801 27.76 3.88 0.70 3.54 23.52 0.28 22.46 0.51 3.56 0.988 0.875 19.79 3.78 0.53 2.28 16.98 0.21 16.17 0.45 2.26 0.987 0.834 24.19 4.08 0.42 2.35 21.42 0.27 20.42 0.43 2.23 0.987 0.838 19.51 3.59 0.36 1.76 17.39 0.40 16.61 0.31 1.67 0.967 0.843 17.94 2.82 0.35 0.55 17.04 5.31 11.66 0.10 0.78 0.687 0.886 27.55 2.51 0.36 1.38 25.81 4.55 20.96 0.24 1.50 0.822 0.862 37.81 1.67 0.47 2.62 34.72 10.03 24.40 0.38 2.62 0.709 0.873 7.69 3.86 0.76 5.14 1.79 1.82 4.13 0.29 1.44 0.694 0.832 3.76 2.21 0.74 2.50 0.52 0.07 2.29 0.11 1.29 0.970 0.921 7.70 3.83 1.19 6.48 0.03 0.40 5.93 0.19 1.18 0.937 0.861 3.57 2.59 0.22 3.35 0.00 0.00 2.54 0.10 0.93 1.000 0.903 5.71 4.16 0.45 5.26 0.00 0.00 4.28 0.15 1.28 1.000 0.895 26.47 3.00 5.08 2.56 18.83 22.70 0.67 0.33 2.77 0.029 0.894 29.17 4.05 0.60 2.00 26.57 26.14 0.47 0.14 2.42 0.018 0.945 19.84 3.35 0.94 1.88 17.02 16.79 0.23 0.20 2.62 0.014 0.929 14.22 1.81 0.33 0.61 13.28 13.10 0.18 0.03 0.91 0.014 0.968 – – – – – – – – – – 17.44 3.12 0.72 1.96 14.76 14.63 0.19 0.22 2.40 0.013 0.916 – – – – – – – – – – – 23.57 3.33 0.62 2.24 20.71 20.42 0.29 0.18 2.68 0.014 0.937 11.92 2.78 0.25 1.43 10.24 10.02 0.22 0.10 1.58 0.021 0.940 16.70 2.87 2.93 1.69 12.08 14.29 0.13 0.13 2.15 0.009 0.943 16.25 2.97 0.21 1.39 14.65 14.45 0.16 0.17 1.40 0.011 0.892 10.16 3.05 0.83 1.78 7.55 7.60 0.08 0.13 2.35 0.010 0.948 – – – – – – – – – – – 16.47 2.95 0.49 1.66 14.32 14.36 0.00 0.12 1.99 0.000 0.943 11.01 2.88 0.36 1.58 9.07 9.24 0.02 0.10 1.65 0.002 0.943 7.27 2.83 0.33 1.80 5.14 5.86 0.00 0.10 1.31 0.000 0.929 9.23 2.30 0.68 1.33 7.22 7.65 0.11 0.07 1.40 0.014 0.952 11.78 4.24 0.74 2.49 8.55 9.38 0.09 0.12 2.19 0.010 0.948 2.83 1.44 0.00 0.76 2.07 2.07 0.08 0.04 0.64 0.037 0.841 6.12 2.55 0.09 2.27 3.76 4.77 0.07 0.10 1.18 0.014 0.922 7.80 3.46 0.70 1.89 5.21 6.02 0.11 0.14 1.53 0.018 0.916 7.49 3.81 0.52 1.57 5.40 6.01 0.21 0.11 1.16 0.034 0.913 – – – – – – – – – – – 15.34 3.11 0.90 2.20 12.23 7.36 5.83 0.21 1.78 0.413 – 8.43 0.73 1.30 1.26 8.66 7.10 7.92 0.14 0.66 0.448 – 2.83 1.44 0.00 0.55 0.00 0.00 0.00 0.03 0.64 0.000 – 37.81 4.24 6.30 6.48 34.72 26.14 24.40 0.57 3.56 1.000 – Quantities are given in μmol g dry wt−1 and are the mean of three extractions of each ecotype. The nos. at the top of each column refer to the list of glucosinolate in “Materials and Methods.” The values underneath the totals section are as follows: Aliph, sum of aliphatic glucosinolates; Indole, sum of indolic glucosinolates; MT, sum of methylthio glucosinolates; MSO, sum of methylsulfinyl glucosinolates; AOP, sum of alkenyl and hydroxy aliphatic glucosinolates; C3, sum of three carbon aliphatic glucosinolates; C4, sum of four carbon aliphatic glucosinolates; C7, sum of seven carbon aliphatic glucosinolates; C8, sum of eight carbon aliphatic glucosinolates; and C4 per, C4/(C3 + C4), C8per = C8/(C7 + C8).
Genotypes 1 2 3 4 5 6 7 E A OH OX OHB Ag-0 4 3 3 2 2 – – 1.02 – – 2.40 0.15 Bla-10 4 3 3 1 2 – – 0.41 – – 1.12 – Bs1 4 3 3 2 2 0.31 – 1.18 2.02 – 8.17 0.29 Cal 4 3 3 2 1 – – – – – 0.46 0.21 Cnt 4 3 3 2 1 – – – 4.20 – 13.66 0.30 Ema-1 4 3 3 2 2 – – 0.58 0.65 – 2.38 – Pog-0 4 3 3 2 1 – – – 2.91 – 7.67 – Tac 4 3 3 2 1 0.19 0.15 – 2.47 – 5.84 0.13 Kas 4 3 2 2 2 0.46 – 0.49 0.42 – 1.43 0.98 Sorbo 4 3 2 2 2 0.37 – 0.89 0.25 – 1.58 – Cvi 4 3 1 2 1 0.36 – – – – – 0.96 Di-1 4 2 – 2 1 0.93 – – – 0.74 – 0.12 Aa-0 4 1 – 2 2 0.26 – 0.81 – 0.52 – – Col 4 1 – 2 2 0.69 – 2.20 – 1.87 – – Ma-0 4 1 – 2 2 0.47 – 1.11 – 0.22 – 0.21 Mt-0 4 1 – 2 2 0.37 – 1.43 – 0.71 – – Can 3 3 – 1 – 0.39 – – – – – 1.64 Kondara 3 3 – 2 – 4.19 0.90 – – – – 6.96 Ei-2 3 3 – 2 – 2.53 – – – – – 4.44 Hodja 3 3 – 2 – 2.27 1.41 – – – – 7.60 Ita-0 3 3 – 2 – 1.83 1.40 – – – – 1.36 Kil-0 3 3 – 2 – 1.36 0.55 – – – – 2.50 Mr-0 3 3 – 2 – 0.84 – – – – 0.13 1.63 Mrk-0 3 3 – 2 – 1.47 0.80 – – – – 1.54 Rsch-0 3 3 – 2 – – – – – – – – Su-0 3 3 – 1 – 0.71 – – 0.13 – 0.64 0.99 Wl-0 3 3 – 2 – 2.17 0.33 – – – – 3.87 Bl-1 3 2 – 2 – 1.88 – – – – – – Di-g 3 2 – 2 – 4.17 – – – – – – Ka-0 3 2 – 2 – 10.91 – – – – – 0.29 Ler 3 2 – 2 – 7.86 0.19 – – – – 0.12 Lip-0 3 2 – 2 – 3.26 – – – – – – No-0 3 2 – 2 – 14.16 0.50 – – 0.28 – – Pet 3 2 – 2 – 8.47 – – – – – – Pi-0 3 2 – 2 – 3.24 – – – – – – Sei-0 3 2 – 2 – 2.58 0.20 – – – – – Tsu-1 3 2 – 2 – 3.61 5.58 – – – – – Yo-0 3 2 – 2 – 2.56 – – – – – 0.16 Oy-0 3 1 – 2 – 1.23 0.62 – – – – – Average – – – – – 2.69 1.05 1.01 1.63 0.72 3.79 1.66 sd – – – – – 3.29 1.49 0.53 1.50 0.60 4.18 2.18 Minimum – – – – – 0.19 0.15 0.41 0.13 0.22 0.13 0.12 Maximum – – – – – 14.16 5.58 2.20 4.20 1.87 13.66 7.60 9 10 11 12 13 14 15 16 17 18 19 20 – 0.25 0.47 – 0.07 – – 0.56 – 0.26 1.72 4.75 – 0.16 0.14 – 0.18 0.80 – 0.28 0.18 0.07 3.74 2.47 – 0.34 0.52 – – – – 0.33 – 0.11 6.86 2.02 – – 0.47 0.25 0.05 – – 0.51 0.11 0.17 1.95 3.41 – 0.49 2.87 0.15 0.15 – – 0.85 – 0.58 2.26 4.55 – 0.46 0.17 – 0.25 – 0.02 0.92 – 0.22 1.52 6.01 – 0.55 0.62 – 0.10 0.08 – 0.77 – 0.29 2.93 5.99 – 0.42 0.65 0.10 – 0.23 – 0.50 – 0.28 4.66 4.56 – – 1.51 – – 0.10 0.03 0.22 – 0.07 0.61 2.99 – – 12.52 – – 0.12 0.02 0.34 – 0.31 3.34 2.72 – – 3.31 0.13 – 0.09 – 0.32 – 0.13 4.01 4.53 – – – – 0.07 0.30 – 0.65 – – 13.09 5.28 – – – – – – 0.02 0.22 – – 11.70 1.90 0.19 – – – 0.07 0.08 0.06 0.55 – – 18.95 2.76 – – – – – 0.22 0.03 0.16 – – 7.66 2.49 – – – – – 0.10 0.01 0.31 – – 12.74 2.49 – – – – – 0.15 – 0.29 0.06 – 0.26 6.81 – – 0.14 – – 3.20 – 0.15 – 0.07 0.27 3.29 – – – – – 0.89 – 0.19 – – 0.17 4.80 – – 0.13 – – 6.85 0.02 0.11 – – 0.26 2.61 – – – – – 9.04 – 0.34 – 0.15 0.72 6.43 – – – – – 3.33 – 0.28 – – 0.11 6.07 – – – – – 0.66 0.02 0.28 – – 0.25 8.19 – – – – – 5.90 – 0.28 – – 0.36 5.83 – – – – – – – – – – – – – – – 0.10 – 0.94 0.02 0.17 – – 0.48 4.17 – – – – – 4.07 – 0.12 – – 0.21 2.46 – – – – – 2.09 0.02 0.16 – – 0.48 4.60 – – – – – 0.69 – 0.20 – – 0.41 4.93 – – – – – 0.52 – 0.14 – – 0.07 3.78 – – – – – 2.76 – 0.11 – – 0.36 3.19 – – – – – 0.41 – 0.09 – – 0.55 1.97 – – – – – 6.81 – 0.16 – – 0.52 2.97 – – – – – 1.20 0.02 0.08 – – 0.48 2.92 – – – – – 0.38 0.02 0.08 – – 0.70 2.30 – – – – – 1.48 – 0.17 – – 0.30 3.38 – – – – – 14.54 – 0.26 – 0.34 0.68 3.70 – – – – – 1.67 0.02 0.12 – – 0.84 1.93 – – – – – 10.78 0.04 0.13 – – 0.37 2.71 – 0.38 1.81 0.15 0.12 2.52 0.02 0.30 0.12 0.22 2.81 3.89 – 0.14 3.38 0.06 0.07 3.57 0.01 0.22 0.06 0.14 4.43 1.58 – 0.16 0.13 0.10 0.05 0.08 0.01 0.08 0.06 0.07 0.07 1.90 – 0.55 12.52 0.25 0.25 14.54 0.06 0.92 0.18 0.58 18.95 8.19 21 23 24 25 27 28 29 30 31 32 33 34 0.41 – 0.08 0.19 0.18 0.86 0.11 3.89 – 12.40 – 0.03 0.08 0.06 0.22 0.04 0.11 0.12 0.07 2.03 0.06 6.54 0.02 0.01 0.12 – 0.88 0.06 0.22 0.56 0.30 2.12 – 4.78 – 0.02 0.24 0.06 0.08 0.11 0.25 0.11 0.08 3.09 0.06 8.31 0.03 0.04 0.41 0.08 0.26 0.03 0.07 0.60 0.04 2.48 0.05 7.56 0.01 0.02 0.29 0.10 0.46 0.05 0.16 0.61 0.22 5.35 – 14.82 – 0.03 0.10 – 0.53 0.06 0.09 1.09 0.21 2.96 0.01 9.15 – 0.04 0.29 – 0.64 0.05 0.12 0.87 0.22 2.25 – 6.80 – 0.04 0.59 – 0.09 0.07 0.60 0.15 0.16 1.57 – 6.24 – 0.03 0.17 – 0.29 0.05 0.25 0.24 – 1.62 – 4.07 – 0.03 0.13 – 0.09 0.06 0.56 – 0.04 1.95 0.02 9.69 – 0.02 0.25 – 0.37 0.14 0.75 – 0.02 2.23 0.01 6.78 – 0.03 0.80 – 0.87 0.04 0.34 – 0.73 1.75 – 3.41 – 0.02 0.80 – 1.05 0.11 0.60 – 0.76 1.36 – 2.69 – 0.05 1.08 – 0.48 0.04 0.23 – 0.75 1.58 – 4.55 0.01 0.04 0.57 – 0.47 0.04 0.48 – 0.64 1.15 – 2.65 – 0.07 0.19 0.09 – 0.04 0.50 – 0.01 2.08 – 14.21 0.01 0.02 0.59 0.06 0.06 0.08 1.33 – 0.01 1.01 – 6.55 – 0.02 0.52 0.10 – 0.07 1.84 0.01 0.01 1.48 – 11.57 – 0.04 0.67 0.07 – 0.04 1.55 – 0.01 0.56 – 3.79 – 0.04 0.35 0.14 0.08 0.08 1.22 – 0.01 1.82 – 16.27 – 0.04 0.14 0.10 – 0.07 1.36 – 0.01 1.39 – 9.24 – 0.04 0.57 0.13 – 0.08 0.90 0.02 – 1.47 – 13.63 0.01 0.03 0.40 0.06 0.07 0.07 1.14 0.01 0.01 1.46 – 12.40 – 0.05 – – – – – – – – – – – – 0.39 0.10 0.10 0.09 0.88 0.03 0.02 1.67 – 11.29 – 0.04 0.09 0.08 – 0.07 1.42 0.01 – 0.66 – 4.89 – 0.05 0.30 0.09 0.07 0.08 1.97 – – 1.39 – 12.62 – 0.02 0.06 0.08 0.07 0.09 1.16 – – 2.18 0.01 13.87 – 0.03 0.35 0.17 0.07 0.10 2.89 – 0.01 1.17 0.01 7.56 – 0.06 0.09 0.07 – 0.09 1.97 – – 0.78 – 7.47 – 0.05 0.16 0.08 0.07 0.09 1.85 – 0.01 1.22 0.01 7.29 – 0.05 0.29 0.10 0.10 0.09 2.39 0.03 0.01 1.06 0.01 5.39 – 0.06 0.35 0.10 0.07 0.09 1.66 – 0.01 1.14 0.01 9.23 0.02 0.03 0.84 0.09 0.07 0.10 1.79 – 0.02 0.92 – 7.66 – 0.05 0.15 0.07 0.16 0.06 1.45 – – 1.11 – 6.69 – 0.04 0.32 0.07 – – 1.11 – – 1.17 – 7.16 – 0.04 0.70 0.10 0.09 0.05 0.77 0.01 0.01 1.24 – 7.56 – 0.03 1.35 – – 0.12 0.65 – – 1.17 – 7.12 – 0.03 0.40 0.09 0.27 0.08 0.97 0.31 0.16 1.72 0.02 8.26 0.02 0.04 0.30 0.03 0.29 0.03 0.73 0.37 0.24 0.92 0.02 3.57 0.01 0.01 0.06 0.06 0.06 0.03 0.07 0.01 0.01 0.56 0.01 2.65 0.01 0.01 1.35 0.17 1.05 0.19 2.89 1.09 0.76 5.35 0.06 16.27 0.03 0.07 Totals Aliph Indole Benzyl MT MSO AOP Benzoxy C3 C4 C7 C8 C4Per C8Per 29.20 0.41 0.19 18.09 5.38 4.30 1.18 0.18 6.58 4.45 17.15 0.973 0.794 18.79 0.08 0.04 13.39 2.93 1.74 0.39 0.91 5.60 2.31 9.01 0.860 0.796 31.03 0.12 0.06 14.64 2.35 12.60 1.10 0.53 19.61 2.45 6.80 0.974 0.735 19.70 0.24 0.11 13.49 3.97 1.31 0.57 0.25 3.07 3.60 11.72 0.925 0.765 41.23 0.41 0.03 12.64 5.55 21.61 0.79 0.07 23.63 3.33 12.11 0.997 0.784 34.91 0.31 0.05 22.25 7.18 4.00 1.02 0.16 6.13 6.27 20.83 0.975 0.769 35.99 0.10 0.06 15.65 6.86 11.49 1.44 0.17 15.43 3.73 15.14 0.989 0.802 31.12 0.29 0.05 14.58 5.21 9.56 1.25 0.69 14.71 2.75 11.36 0.855 0.805 18.12 0.62 0.07 8.61 3.21 5.36 0.94 1.16 4.77 1.79 9.23 0.804 0.838 28.94 0.19 0.05 9.44 3.06 15.92 0.52 0.74 18.82 1.96 6.79 0.962 0.776 26.21 0.13 0.06 15.83 4.85 4.76 0.64 1.01 7.36 2.27 14.22 0.879 0.862 31.37 0.25 0.14 22.77 6.74 1.05 0.81 1.98 13.85 2.88 12.06 0.875 0.807 22.53 0.82 0.04 17.73 2.64 1.07 1.09 0.60 13.76 1.97 5.31 0.958 0.729 33.87 0.86 0.11 24.13 5.44 2.89 1.41 1.37 23.78 1.91 5.45 0.946 0.740 20.18 1.11 0.04 14.49 2.87 1.79 1.03 0.92 9.74 1.74 7.04 0.914 0.802 23.61 0.58 0.04 17.11 3.51 1.80 1.19 0.95 15.52 1.46 5.14 0.942 0.779 26.52 0.19 0.04 16.79 7.10 2.03 0.54 1.04 0.27 2.37 21.02 0.206 0.899 28.21 0.59 0.08 11.15 4.34 11.36 1.36 9.62 0.42 1.16 9.84 0.042 0.895 28.07 0.52 0.07 14.21 4.99 6.97 1.90 5.26 0.19 1.67 16.37 0.035 0.907 27.26 0.69 0.04 11.53 4.13 10.00 1.60 12.08 0.40 0.67 6.40 0.032 0.905 40.85 0.35 0.08 28.07 8.17 3.34 1.27 13.49 0.73 2.16 22.70 0.051 0.913 26.34 0.14 0.07 14.17 6.90 3.86 1.41 6.60 0.12 1.67 15.31 0.018 0.902 28.17 0.59 0.08 16.14 8.47 2.60 0.96 2.40 0.40 1.75 21.82 0.143 0.926 31.38 0.40 0.07 20.25 6.91 3.01 1.21 9.31 0.38 1.74 18.23 0.039 0.913 – – – – – – – – – – – – – 22.46 0.41 0.09 14.58 4.34 2.47 0.97 2.53 1.30 1.84 15.46 0.339 0.894 20.34 0.09 0.07 9.91 2.91 6.04 1.48 7.99 0.22 0.78 7.35 0.027 0.904 25.37 0.32 0.08 16.74 4.76 1.88 1.99 5.94 0.48 1.55 17.22 0.075 0.917 27.80 0.06 0.09 17.30 5.13 4.17 1.20 6.02 0.41 2.38 18.80 0.064 0.888 27.65 0.35 0.10 9.56 3.92 11.20 2.97 14.32 0.08 1.31 11.34 0.006 0.896 24.93 0.09 0.09 11.44 3.49 7.98 2.02 12.78 0.36 0.89 10.66 0.027 0.923 16.86 0.16 0.09 9.62 2.06 3.26 1.92 5.52 0.56 1.31 9.26 0.092 0.876 34.55 0.29 0.09 13.98 3.91 14.16 2.50 23.86 0.84 1.22 8.36 0.034 0.873 25.42 0.37 0.09 12.22 3.00 8.47 1.73 11.33 0.49 1.22 12.15 0.041 0.909 17.30 0.86 0.10 9.82 2.38 3.24 1.86 5.41 0.72 1.00 9.96 0.117 0.909 17.63 0.15 0.06 9.81 3.75 2.58 1.49 5.71 0.30 1.28 10.07 0.050 0.887 38.26 0.32 0.00 23.62 9.54 3.95 1.15 24.84 0.68 1.43 10.86 0.027 0.884 17.09 0.72 0.05 11.50 2.05 2.72 0.82 5.00 0.86 1.36 9.49 0.147 0.875 24.81 1.39 0.12 19.44 3.46 1.23 0.68 13.28 0.37 1.30 9.83 0.027 0.883 26.95 0.41 0.07 15.18 4.67 5.73 1.27 5.68 5.60 2.02 12.15 0.436 0.852 6.57 0.31 0.03 4.67 1.92 4.83 0.56 6.34 7.44 1.10 4.92 0.435 0.062 16.86 0.06 0.00 8.61 2.05 1.05 0.39 0.07 0.08 0.67 5.14 0.006 0.729 41.23 1.39 0.19 28.07 9.54 21.61 2.97 24.84 23.78 6.27 22.70 0.997 0.926 Quantities are given in μmol g dry wt−1 and are the mean of three extractions of each ecotype. The nos. at the top of each column refer to the list of glucosinolate in “Materials and Methods.” The values underneath the totals section are as follows: Aliph, sum of aliphatic glucosinolates; Indole, sum of indolic glucosinolates; Benzyl, sum of benzyl glucosinolates; MT, sum of methylthioalkyl glucosinolates; MSO, sum of methylsulfinyl glucosinolates; AOP, sum of alkenyl and hydroxy aliphatic glucosinolates; Benzoxy, sum of benzoyloxy aliphatic glucosinolates; C3, sum of three carbon aliphatic glucosinolates; C4, sum of four carbon aliphatic glucosinolates; C7, sum of seven carbon aliphatic glucosinolates; C8, sum of eight carbon aliphatic glucosinolates; and C4 per, C4/(C3 +C4), C8 per = C8/(C7 + C8).
Ecotype Elong AOP OX OH Profile Ag-0 4 3 2 3 Bla-10 4 3 1 3 Bs-1 4 3 2 3 Allyl Cal-0 4 3 2 3 Butenyl Cnt 4 3 2 3 HydroxyButenyl Ema-1 4 3 2 3 Tac 4 3 2 3 Pog-0 4 3 2 3 Sorbo 4 3 2 2 Kas 4 3 2 2 Allyl Cvi 4 3 2 1 Butenyl Di-1 4 2 2 – OHPropyl/4MSO Aa-0 4 1 2 – Col-0 4 1 2 – 4MSO-butyl Ma-0 4 1 2 – Mt-0 4 1 2 – Can-0 3 3 1 – Kondara 3 3 2 – Ei-2 3 3 2 – Hodja 3 3 2 – Ita-0 3 3 2 – Allyl Kil-0 3 3 2 – Mr-0 3 3 2 – Mrk-0 3 3 2 – Su-0 3 3 1 – Wi-0 3 3 2 – Rsch-0 3 3 2 – Bl-1 3 2 2 – Di-g 3 2 2 – Ka-0 3 2 2 – Ler 3 2 2 – Lip-0 3 2 2 – OHpropyl No-0 3 2 2 – Pet 3 2 2 – Pi-0 3 2 2 – Sei-0 3 2 2 – Tsu-1 3 2 2 – Yo-0 3 2 2 – Oy-0 3 1 2 – 3MSOpropyl Shown are the genotypes at the five major glucosinolate biosynthetic genetic loci for the 39 ecotypes analyzed in this study. The allele designations are as follows: GS-Elong, 3, C3 producing; 4, C4 producing; GS-AOP, 1, the null methylsulfinylalkyl allele; 2, the hydroxypropyl allele; 3, the alkenyl allele; GS-OX, 1, the Bla-10 allele; 2, the normal wild-type allele; GS-OH, 1, off in all tissues; 2, only on in seeds; and 3, on in all tissues. Ecotypes with no score do not make the necessary precursor for the reaction and are thereby unable to be scored for the allele at that specific locus.
