Article Figures & Data
Figures
- Fig. 1.
A, RT-PCR analysis of mRNA accumulation of anthocyanin structural and regulatory genes in r-ΔSn1-bol3 pl-bol3 seedlings exposed to continuous white, blue, red, UV-A, and UV-B light for 0, 24, 48, or 72 h. cDNA was made from total RNA extracted from mesocotyls. Specific primers for the Sn, pl, C2, and A1 genes were used to amplify the cDNA (see “Materials and Methods”). Amplifications were carried out for 20 cycles. The amplification of the orp-1 transcript was used as an internal control. The blots were hybridized with the different probes (see “Materials and Methods”). B, Anthocyanin accumulation in mesocotyl was measured in each treatment as described in A. Values are expressed as A 530 per mesocotyl. Mean values represent 10 independent replicates. Meanses are below 5%.
- Fig. 2.
A, RT-PCR analysis of mRNA accumulation of anthocyanin structural and regulatory genes in R-sc sn C1 aleurones of 30-d after pollination seeds exposed to continuous white, blu,e and red light for 0, 24, 48, or 72 h. cDNA was made from total RNA extracted from aleurones. Specific primers for theR, C1, and A1 genes were used to amplify the cDNA (see “Materials and Methods”). Amplifications were carried out for 20 cycles. The amplification of theorp-1 transcript was used as an internal control. The blots were hybridized with the different probes (see “Materials and Methods”). B, Anthocyanin accumulation in aleurones was measured in each treatment as described in A. Values are expressed asA 530 per aleurone. Mean values represent 10 independent replicates. Mean ses are below 5%.
- Fig. 3.
RT-PCR analysis of mRNA accumulation of anthocyanin structural and regulatory genes in r-Δ Sn1-bol3 pl-bol3 seedlings treated with a 25 μm BA solution for 7 d in darkness and subsequently exposed to continuous white light for 0, 24, and 48 h. Control plants were maintained in the same conditions. cDNA was made from total RNA extracted from mesocotyls. Specific primers for theSn, pl, and A1 genes were used to amplify the cDNA (see “Materials and Methods”). Amplifications were carried out for 20 cycles. The amplification of theorp-1 transcript was used as an internal control. The blots were hybridized with the different probes (see “Materials and Methods”).
Tables
- Table I.
Anthocyanin accumulation in the mesocotyl of 5-d-old dark-grown r-Δ Sn1-bol3 pl-bol3 and r-Δ Sn1-bol3 pl-W22 seedlings after 72 h subsequent treatment with different light quality
Line Light Treatment Dark White Blue Red UV-A UV-B pl-bol3 0.04 9.01 4.88 0.86 2.42 6.98 pl-W22 0.08 1.17 1.22 0.11 0.41 1.31 Mean values are expressed as A 530 per mesocotyl. Mean ses are below 5%. For light treatments, see “Materials and Methods.”
- Table II.
Anthocyanin accumulation in the mesocotyl of r-Δ Sn1-bol3 pl-bol3 and r-Δ Sn1-bol3 pl-W22 plantlets grown for 10 d in the dark with BA at 0, 0.5, and 25 μm, followed by 2 d in the light
Line BA Treatment Control 0.5 25 μm pl-bol3 0.47 0.78 2.32 pl-W22 0.11 0.11 0.12 Mean values are expressed as A 530 per mesocotyl. Mean ses are below 5%. For hormone treatments, see “Materials and Methods.”
