Article Figures & Data
Figures
- Fig. 1.
RNA gel-blot profile for tomato root MAP kinase induced by deprivation of Pi, K, or Fe. The MAP kinase is homologous to tobacco WIPK and is rapidly induced by withholding Pi, K, and Fe from the plant. A, RNA gel blot. In this and subsequent experiments, RNA abundance for roots of treated plants was compared with gel blots for roots of control (nutrient-sufficient) plants analyzed at the same time points to ensure that the changes in transcript abundance were not attributable to a diurnal response. In none of the experiments did the control plants show significant changes in gene expression over the 48-h time period used for these experiments. B, Quantitative expression data for the RNA gel blot depicted in A. In Figure 1B and the subsequent figures (except Fig. 4), the autoradiograph of the RNA gel blot was scanned, and the image was computer digitized. The gel-blot intensities were quantified using the NIH ImageJ program. Changes in gene expression were quantified as a relative signal ratio, which was determined by dividing the quantified gel-blot intensity at each time point by the RNA-blot intensity for the 0-h nutrient-sufficient plants.
- Fig. 2.
Up-regulation of the tomato MAP kinase kinase,MEK1, induced by Pi, K, or Fe deprivation. A, RNA gel blot. B, Quantitative expression data for the RNA gel blot depicted in A.
- Fig. 3.
The transcription factor Nitf was induced by Pi, K, and Fe deprivation in tomato roots. A, RNA gel blot. B, Quantitative expression data for the RNA gel blot depicted in A.
- Fig. 4.
Tissue-specific expression of the transcription factor, Nitf, in different tissues and organs of tomato seedlings grown under nutrient-sufficient hydroponical conditions. Lanes 1 and 2, Roots. Because the root system was relatively large, different parts of the roots were harvested and bulked. Lane 3, Older leaves harvested from below middle trusse of the plants. These are completely functioning leaves, healthy, not showing any aging symptoms. Lane 4, Young leaves harvested from the top of the plants. They were barely fully expanded. Lane 5, Flowers. Lane 6, Old stems. Main stems harvested from bottom part of the plants, directly above the root. Lane 7, Young stems. Main stems harvested from the top part of the plants. Lane 8, Unripe fruits. Green tomato fruits of various sizes. All tissues were harvested from the same plants at the same physiological age.
- Fig. 5.
RNA gel blot for a gene encoding a 14-3-3 protein induced by Pi, K, and Fe deprivation. A, RNA gel blot. B, Quantitative expression data for the RNA gel blot depicted in A.
- Fig. 6.
Expression of the tomato Pi transporter,LePT1, was strongly up-regulated in roots of plants subjected to Pi, K, and Fe deprivation. A, RNA gel blot. B, Quantitative expression data for the RNA gel blot depicted in A.
- Fig. 7.
Expression of a K+ channel homolog of carrot Kdc1, LeKC1 was up-regulated by Pi, K, and Fe deprivation. A, RNA gel blot. B, Quantitative expression data for the RNA gel blot depicted in A.
- Fig. 8.
Expression of the tomato Fe transporter,LeIRT1, was up-regulated by Pi, K, and Fe deprivation. A, RNA gel blot. B, Quantitative expression data for the RNA gel blot depicted in A.
- Fig. 9.
Expression of Nitf to Pi, K, and Fe deprivation in decapitated plants. Control plant is the same RNA blot run with RNA from roots of decapitated plants under nutrient-sufficient conditions. A, RNA gel blot. B, α-Tubulin loading control.
Tables
- Table I.
Genes induced by Pi, K, or Fe deficiencies in tomato roots and confirmed by northern analysis
Clone ID Gene (Induction) Homology/Function Accession No. GenBank Hit Reference D22-1 MAP kinase (up 1–3 h) Tobacco WIPK/signaling AW979631 D61377 Seo et al. (1995) H10-1 Nitf (up 1–6 h) Tobacco TGA1a/regulatory AAK71287 X16450 Katagiri et al. (1989) K20-1 MEK1 (up 1 h) Tomato gene/signaling AW220008 CAA04261 Hackett et al. (1998) O22-1 14-3-3 (up 3–12 h) Tomato gene/signaling AW218217 AF079450 X95905 Roberts and Bowles (1999); Pnueli et al. (2001) 27L1 LePT1 (up 3–12 h) Tomato gene/Pi transporter AF022873 AF022873 Liu et al. (1998) E13-1 LeHAK5 (up 1 h) Arabidopsis HAK5 /K+transporter AW979921 AF129478 G22-1 LeKC1 (up 3–12 h) Carrot Kdc1/K+channel AW218955 AJ249962 Downey et al. (2000) I20-1 LeIRT2 (3–24 h) Tomato gene/iron transporter AW219515 AF246266 Eckhardt et al. (2001) 1A16 Nicotianamine synthase (up 3–6 h) Tomato gene /nicotianamine biosynthesis BG791292 CAB42052 Ling et al. (1999) N5-2 Glu decarboxylase (up 1–6 h) Tobacco GAD/GABA biosynthesis BG713805 AAB40608 I17-2 Enolase (up 1–12 h) Tomato gene/glycolyis BG791245 X58108 Van der Straeten et al. (1991) A4-4 ATHP3 (up 1–6 h) Arabidopsis ATHP3 /phosphorelay mediator BG791212 BAA37112 Miyata et al. (1998) D11-1 ERF3 (up 1–3 h) Tobacco ERF3 /regulatory AW979604 AB024575 G20-2 SKD1 (up 3–24 h) Human SKD1 /membrane transport AW621245 AAF17203 Scheuring et al. (1999) G19-1 bet3 (up 12–48 h) Mouse bet3/vesicular transport AW218945 AF041433 Jiang et al. (1998) N3-1 dwarf1 (down after 1 h) Arabidopsis dwarf1/cell elongation BG791226 U12400 Takahashi et al. (1995) B2-1 MAP kinase 6 (up 3–6 h) Tobacco MAPK 6/signaling AW980007 X83879 Wilson et al. (1995) Except for dwarf1, all other genes were up-regulated by nutrient treatments. See text for details.
