Article Figures & Data
Figures
- Fig. 1.
Autoradiogram of TLC-separated, labeled products of endo-β-mannanase digestion of 70% (v/v) methanol-insoluble products formed on incubating microsomal membrane preparations from developing tobacco seeds with GDP-(14C)-Man (80 μm) and unlabeled UDP-Gal (0.8 mm). M, Man; O, galactomannan octasaccharides; N, galactomannan nonasaccharides; H, higher galactomannan oligosaccharides.
- Fig. 2.
TLC separation of the saccharides released on treating total endosperm cell wall materials with the pure endo-(1→4)-β-mannanase from A. niger. C, Endosperms from control seeds (CTR B). T1, T1 generation seeds (self-pollination) from primary transformant FGT 6. T2, T2 generation seeds derived from FGT 6. Statistically, the T1 generation seeds will include 25% azygous individuals lacking the transgene, and the T2 generation seeds will include less than 3% azygous individuals. M, Man; O, galactomannan octasaccharides; N, galactomannan nonasaccharides; H, higher galactomannan oligosaccharides.
- Fig. 3.
Autoradiogram of TLC-separated, labeled products of endo-β-mannanase digestion of 70% (v/v) methanol-insoluble products formed on incubating microsomal membrane preparations from developing tobacco seeds with GDP-(14C)-Man (80 μm) and unlabeled UDP-Gal (0.8 mm). C, Membranes from developing seeds of control plant (CTR A, self pollination) 20 d after anthesis. T1, Membranes from developing seeds of primary transformant FGT 3 (T1 generation, self-pollination) 20 d after anthesis. M, Man; O, galactomannan octasaccharides; N, galactomannan nonasaccharides; H, higher galactomannan oligosaccharides.
Tables
- Table II.
Treatment of tobacco endosperm cell wall material with A. niger endo-(1→4)-β-mannanase
Composition (mol % of Fraction) Digestion Time Fraction (D or R) % of Total Ara Gal Glc Man h 1 D 36 1.2 3.8 5.9 89 1 R 64 28 14 5.5 532-a 4 D 49 2.3 3.7 5.6 88 4 R 51 36 16 5.6 422-a 16 D 57 2.7 4.1 6.2 87 16 R 43 34 15 7.1 442-a Relative amounts and compositions of the cell wall fractions depolymerized by (D) and resistant to (R) enzyme digestion.
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↵F2-a Contained some Xyl (<10%).
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Days after Anthesis Capsule Fresh Wt Capsule Color Calyx Color Seed Fresh Wt Seed Color Man as % of Seed Cell Walls g g 14 0.34 gr. l. gr. 0.04 Colorless 0 15 0.56 gr. l. gr. 0.06 Colorless 0 17 0.96 d. gr. gr. 0.19 y. 5 19 1.13 d. gr. gr. 0.21 y./br. 23 20 1.20 y./gr. y./gr. 0.20 br./d. br 28 tip br. 21 1.23 y./gr. y./gr. 0.22 br./d. br. 29 tip br. 22 1.29 y./gr. y./gr. 0.26 d. br. 34 tip br. 23 1.08 y./gr. y. 0.19 d. br (dry) 38 tip br. gr, Green; y, yellow; br, brown. d, dark; l, light.
- Table IV.
Compositions of total cell wall materials from endosperm tissues of T1 and T2 generation seeds from transgenic tobacco plants expressing fenugreek GMGT
Transgenic or Control Line Composition Man/Gal Value4-b Ara Gal Glc Xyl4-a Man mol % T2generation FGT 1 17.0 17.0 4.4 – 61.7 3.5 16.1 15.1 4.4 – 64.3 4.1 FGT 6 20.1 16.0 4.2 2.8 56.8 3.6 19.8 16.1 4.0 2.4 57.7 3.6 FGT AA 20.1 15.9 3.6 2.4 58.0 3.6 18.6 14.5 12.0 2.2 52.6 3.6 T1generation FGT 1 17.3 10.8 4.8 2.7 64.5 6.0 17.7 11.7 4.8 – 65.8 5.4 FGT 3 17.3 11.5 5.4 – 65.8 5.5 17.6 11.7 5.3 – 65.4 5.4 FGT 6 16.7 11.6 5.8 – 65.9 5.5 18.2 12.4 5.9 2.9 60.6 4.9 FGT O 16.9 11.9 5.4 – 65.8 5.3 18.8 14.3 5.0 – 61.9 4.2 FGT L 18.2 12.3 5.0 – 64.5 5.0 17.6 11.8 5.3 – 65.4 5.3 FGT AA 18.6 14.9 3.8 – 62.6 4.0 18.9 15.2 4.8 – 61.0 3.8 Controls CTR A 18.4 10.2 3.5 – 67.9 6.4 19.5 10.0 4.0 2.4 64.2 6.4 CTR B 18.4 9.2 4.5 – 67.8 7.1 17.8 9.3 5.4 – 67.5 7.0 CTR C 19.0 10.2 3.8 2.4 64.5 6.3 17.7 10.2 3.7 – 68.3 6.4 The T1 generation seeds include 25% azygous individuals without the transgene, whereas the T2 generation seeds include less than 3% of such individuals. Transgenics were independent lines, and control plants had been regenerated from tissue culture. Duplicate analyses are of independent batches of 50 seeds.
- Table V.
Amounts and compositions of cell wall materials dissolved by the action of endo-(1→4)-β-d-mannanase on endosperm tissues of T1 and T2 generation seeds from transgenic tobacco plants expressing fenugreek GMGT
Composition5-a Transgenic or Control Line Ara Gal Glc Man Man/Gal Value % of Wall Dissolved % of Man Dissolved mol % T2generation FGT 1 3.3 10.3 6.6 79.8 7.7 54 70 4.2 10.4 8.1 77.3 7.4 51 69 FGT 6 4.8 9.9 11.1 74.1 7.5 58 78 5.0 10.1 9.0 76.0 7.5 60 80 FGT AA 3.9 10.4 6.2 79.6 7.7 56 75 4.3 10.7 6.1 78.8 7.4 55 77 T1 generation FGT 1 4.4 7.0 6.9 81.7 11.7 62 805-b 4.2 7.1 6.5 82.1 11.5 63 855-b FGT 3 4.1 7.4 6.7 81.8 11.1 61 805-b 4.0 7.8 7.1 81.1 10.4 63 805-b FGT 6 4.0 7.5 6.9 81.5 10.9 58 755-b 3.6 7.3 7.0 82.1 11.2 60 805-b FGT O 3.6 7.4 7.5 81.5 11.0 58 75 4.3 7.2 7.2 81.2 11.2 57 74 FGT L 3.7 8.3 7.1 80.8 9.7 52 71 5.0 8.1 6.4 80.6 10.0 62 79 FGT AA 4.2 8.3 6.0 81.5 9.8 54 74 4.4 8.8 5.9 80.9 9.2 51 705-b Controls CTR A 2.7 4.4 6.7 86.2 19.6 58 805-b 2.8 4.2 5.6 87.4 20.8 62 84 CTR B 3.3 4.1 5.9 86.8 21.2 69 87 2.9 4.0 5.6 87.6 21.9 63 855-b CTR C 3.1 4.2 5.2 87.5 20.8 61 82 3.7 4.5 5.8 86.1 19.1 60 82 The T1 generation seeds include 25% azygous individuals without the transgene, whereas the T2 generation seeds include less than 3% of such individuals. Transgenics were independent lines, and control plants had been regenerated from tissue culture. Duplicate analyses are of independent lots of 50 endosperms.
