Skip to main content

Main menu

  • For Authors
    • Submit a Manuscript
    • Instructions for Authors
  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
    • Focus Collections
    • Classics Collection
    • Upcoming Focus Issues
  • Advertisers
  • About
    • About the Journal
    • Editorial Board and Staff
  • Subscribers
  • Librarians
  • More
    • Alerts
    • Contact Us
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Plant Cell Teaching Tools
    • ASPB
    • Plantae

User menu

  • My alerts
  • Log in

Search

  • Advanced search
Plant Physiology
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Plant Cell Teaching Tools
    • ASPB
    • Plantae
  • My alerts
  • Log in
Plant Physiology

Advanced Search

  • For Authors
    • Submit a Manuscript
    • Instructions for Authors
  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
    • Focus Collections
    • Classics Collection
    • Upcoming Focus Issues
  • Advertisers
  • About
    • About the Journal
    • Editorial Board and Staff
  • Subscribers
  • Librarians
  • More
    • Alerts
    • Contact Us
  • Follow plantphysiol on Twitter
  • Visit plantphysiol on Facebook
  • Visit Plantae
Research ArticleBIOCHEMICAL PROCESSES AND MACROMOLECULAR STRUCTURES
You have accessRestricted Access

Tobacco Transgenic Lines That Express Fenugreek Galactomannan Galactosyltransferase Constitutively Have Structurally Altered Galactomannans in Their Seed Endosperm Cell Walls

J.S. Grant Reid, Mary E. Edwards, Cathryn A. Dickson, Catherine Scott, Michael J. Gidley
J.S. Grant Reid
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mary E. Edwards
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Cathryn A. Dickson
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Catherine Scott
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Michael J. Gidley
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site

Published March 2003. DOI: https://doi.org/10.1104/pp.102.016840

  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Article Figures & Data

Figures

  • Tables
  • Fig. 1.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Fig. 1.

    Autoradiogram of TLC-separated, labeled products of endo-β-mannanase digestion of 70% (v/v) methanol-insoluble products formed on incubating microsomal membrane preparations from developing tobacco seeds with GDP-(14C)-Man (80 μm) and unlabeled UDP-Gal (0.8 mm). M, Man; O, galactomannan octasaccharides; N, galactomannan nonasaccharides; H, higher galactomannan oligosaccharides.

  • Fig. 2.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Fig. 2.

    TLC separation of the saccharides released on treating total endosperm cell wall materials with the pure endo-(1→4)-β-mannanase from A. niger. C, Endosperms from control seeds (CTR B). T1, T1 generation seeds (self-pollination) from primary transformant FGT 6. T2, T2 generation seeds derived from FGT 6. Statistically, the T1 generation seeds will include 25% azygous individuals lacking the transgene, and the T2 generation seeds will include less than 3% azygous individuals. M, Man; O, galactomannan octasaccharides; N, galactomannan nonasaccharides; H, higher galactomannan oligosaccharides.

  • Fig. 3.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Fig. 3.

    Autoradiogram of TLC-separated, labeled products of endo-β-mannanase digestion of 70% (v/v) methanol-insoluble products formed on incubating microsomal membrane preparations from developing tobacco seeds with GDP-(14C)-Man (80 μm) and unlabeled UDP-Gal (0.8 mm). C, Membranes from developing seeds of control plant (CTR A, self pollination) 20 d after anthesis. T1, Membranes from developing seeds of primary transformant FGT 3 (T1 generation, self-pollination) 20 d after anthesis. M, Man; O, galactomannan octasaccharides; N, galactomannan nonasaccharides; H, higher galactomannan oligosaccharides.

Tables

  • Figures
    • View popup
    Table I.

    Neutral monosaccharide composition of cell wall materials from tobacco tissues

    TissueAra1-aGalGlcXylMan
    mol %
    Leaf midrib5568175
    Seed testa19923462
    Seed endosperm15916358
    Seed embryo2974123<1
    • ↵F1-a Includes Rha.

    • View popup
    Table II.

    Treatment of tobacco endosperm cell wall material with A. niger endo-(1→4)-β-mannanase

    Composition (mol % of Fraction)
    Digestion TimeFraction (D or R)% of TotalAraGalGlcMan
    h
    1D361.23.85.989
    1R6428145.5532-a
    4D492.33.75.688
    4R5136165.6422-a
    16D572.74.16.287
    16R4334157.1442-a

    Relative amounts and compositions of the cell wall fractions depolymerized by (D) and resistant to (R) enzyme digestion.

      • ↵F2-a Contained some Xyl (<10%).

      • View popup
      Table III.

      Development of tobacco fruits in relation to mannan deposition

      Days after AnthesisCapsule Fresh WtCapsule ColorCalyx ColorSeed Fresh WtSeed ColorMan as % of Seed Cell Walls
      g g
      140.34gr.l. gr.0.04Colorless0
      150.56gr.l. gr.0.06Colorless0
      170.96d. gr.gr.0.19y.5
      191.13d. gr.gr.0.21y./br.23
      201.20y./gr.y./gr.0.20br./d. br28
      tip br.
      211.23y./gr.y./gr.0.22br./d. br.29
      tip br.
      221.29y./gr.y./gr.0.26d. br.34
      tip br.
      231.08y./gr.y.0.19d. br (dry)38
      tip br.

      gr, Green; y, yellow; br, brown. d, dark; l, light.

        • View popup
        Table IV.

        Compositions of total cell wall materials from endosperm tissues of T1 and T2 generation seeds from transgenic tobacco plants expressing fenugreek GMGT

        Transgenic or Control LineCompositionMan/Gal Value4-b
        AraGalGlcXyl4-aMan
        mol %
        T2generation
         FGT 117.017.04.4–61.73.5
        16.115.14.4–64.34.1
         FGT 620.116.04.22.856.83.6
        19.816.14.02.457.73.6
         FGT AA20.115.93.62.458.03.6
        18.614.512.02.252.63.6
        T1generation
         FGT 117.310.84.82.764.56.0
        17.711.74.8–65.85.4
         FGT 317.311.55.4–65.85.5
        17.611.75.3–65.45.4
         FGT 616.711.65.8–65.95.5
        18.212.45.92.960.64.9
         FGT O16.911.95.4–65.85.3
        18.814.35.0–61.94.2
         FGT L18.212.35.0–64.55.0
        17.611.85.3–65.45.3
         FGT AA18.614.93.8–62.64.0
        18.915.24.8–61.03.8
        Controls
         CTR A18.410.23.5–67.96.4
        19.510.04.02.464.26.4
         CTR B18.49.24.5–67.87.1
        17.89.35.4–67.57.0
         CTR C19.010.23.82.464.56.3
        17.710.23.7–68.36.4

        The T1 generation seeds include 25% azygous individuals without the transgene, whereas the T2 generation seeds include less than 3% of such individuals. Transgenics were independent lines, and control plants had been regenerated from tissue culture. Duplicate analyses are of independent batches of 50 seeds.

          • ↵F4-a Where not listed, included in Man total.

          • ↵F4-b Where independent figure for Xyl not given, 2.6 mol % Xyl assumed

          • View popup
          Table V.

          Amounts and compositions of cell wall materials dissolved by the action of endo-(1→4)-β-d-mannanase on endosperm tissues of T1 and T2 generation seeds from transgenic tobacco plants expressing fenugreek GMGT

          Composition5-a
          Transgenic or Control LineAraGalGlcManMan/Gal Value% of Wall Dissolved% of Man Dissolved
          mol %
          T2generation
           FGT 13.310.36.679.87.75470
          4.210.48.177.37.45169
           FGT 64.89.911.174.17.55878
          5.010.19.076.07.56080
           FGT AA3.910.46.279.67.75675
          4.310.76.178.87.45577
          T1 generation
           FGT 14.47.06.981.711.762805-b
          4.27.16.582.111.563855-b
           FGT 34.17.46.781.811.161805-b
          4.07.87.181.110.463805-b
           FGT 64.07.56.981.510.958755-b
          3.67.37.082.111.260805-b
           FGT O3.67.47.581.511.05875
          4.37.27.281.211.25774
           FGT L3.78.37.180.89.75271
          5.08.16.480.610.06279
           FGT AA4.28.36.081.59.85474
          4.48.85.980.99.251705-b
          Controls
           CTR A2.74.46.786.219.658805-b
          2.84.25.687.420.86284
           CTR B3.34.15.986.821.26987
          2.94.05.687.621.963855-b
           CTR C3.14.25.287.520.86182
          3.74.55.886.119.16082

          The T1 generation seeds include 25% azygous individuals without the transgene, whereas the T2 generation seeds include less than 3% of such individuals. Transgenics were independent lines, and control plants had been regenerated from tissue culture. Duplicate analyses are of independent lots of 50 endosperms.

            • ↵F5-a Xyl absent from dissolved cell wall material.

            • ↵F5-b Less precise value due to poor separation of Xyl from Man in hydrolysate of undissolved cell wall material.

          PreviousNext
          Back to top

          Table of Contents

          Print
          Download PDF
          Email Article

          Thank you for your interest in spreading the word on Plant Physiology.

          NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

          Enter multiple addresses on separate lines or separate them with commas.
          Tobacco Transgenic Lines That Express Fenugreek Galactomannan Galactosyltransferase Constitutively Have Structurally Altered Galactomannans in Their Seed Endosperm Cell Walls
          (Your Name) has sent you a message from Plant Physiology
          (Your Name) thought you would like to see the Plant Physiology web site.
          CAPTCHA
          This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
          Citation Tools
          Tobacco Transgenic Lines That Express Fenugreek Galactomannan Galactosyltransferase Constitutively Have Structurally Altered Galactomannans in Their Seed Endosperm Cell Walls
          J.S. Grant Reid, Mary E. Edwards, Cathryn A. Dickson, Catherine Scott, Michael J. Gidley
          Plant Physiology Mar 2003, 131 (3) 1487-1495; DOI: 10.1104/pp.102.016840

          Citation Manager Formats

          • BibTeX
          • Bookends
          • EasyBib
          • EndNote (tagged)
          • EndNote 8 (xml)
          • Medlars
          • Mendeley
          • Papers
          • RefWorks Tagged
          • Ref Manager
          • RIS
          • Zotero
          Request Permissions
          Share
          Tobacco Transgenic Lines That Express Fenugreek Galactomannan Galactosyltransferase Constitutively Have Structurally Altered Galactomannans in Their Seed Endosperm Cell Walls
          J.S. Grant Reid, Mary E. Edwards, Cathryn A. Dickson, Catherine Scott, Michael J. Gidley
          Plant Physiology Mar 2003, 131 (3) 1487-1495; DOI: 10.1104/pp.102.016840
          del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
          • Tweet Widget
          • Facebook Like
          • Google Plus One

          Jump to section

          • Article
            • Abstract
            • RESULTS
            • DISCUSSION
            • MATERIALS AND METHODS
            • ACKNOWLEDGMENT
            • Footnotes
            • LITERATURE CITED
          • Figures & Data
          • Info & Metrics
          • PDF

          In this issue

          Plant Physiology: 131 (3)
          Plant Physiology
          Vol. 131, Issue 3
          Mar 2003
          • Table of Contents
          • About the Cover
          • Index by author
          View this article with LENS

          More in this TOC Section

          • Decreasing the Mitochondrial Synthesis of Malate in Potato Tubers Does Not Affect Plastidial Starch Synthesis, Suggesting That the Physiological Regulation of ADPglucose Pyrophosphorylase Is Context Dependent
          • UDP-Glycosyltransferases from the UGT73C Subfamily in Barbarea vulgaris Catalyze Sapogenin 3-O-Glucosylation in Saponin-Mediated Insect Resistance
          • Acyl Editing and Headgroup Exchange Are the Major Mechanisms That Direct Polyunsaturated Fatty Acid Flux into Triacylglycerols
          Show more BIOCHEMICAL PROCESSES AND MACROMOLECULAR STRUCTURES

          Similar Articles

          Our Content

          • Home
          • Current Issue
          • Plant Physiology Preview
          • Archive
          • Focus Collections
          • Classic Collections
          • The Plant Cell
          • Plant Direct
          • Plantae
          • ASPB

          For Authors

          • Instructions
          • Submit a Manuscript
          • Editorial Board and Staff
          • Policies
          • Recognizing our Authors

          For Reviewers

          • Instructions
          • Journal Miles
          • Policies

          Other Services

          • Permissions
          • Librarian resources
          • Advertise in our journals
          • Alerts
          • RSS Feeds

          Copyright © 2021 by The American Society of Plant Biologists

          Powered by HighWire