Article Figures & Data
Figures
- Fig. 1.
Effect of conventional cold acclimation on the survival of protoplasts isolated from leaves of seedlings ofsfr4 mutant and wild type. ○, Nonacclimated (NA)sfr4 protoplasts; ●, 7-d-cold-acclimated (ACC)sfr4 protoplasts; ■, NA wild-type protoplasts; ▪, 7-d-ACC wild-type protoplasts. Results (percent survival) are plotted relative to the survival of unfrozen controls. Each result indicates the mean and sd of at least two different experiments, with two samples taken per experiment and three counts performed on each sample. Absence of sd values indicates that they are smaller than the size of the symbols.
- Fig. 2.
Effect of treatments on the incidence of EIL (left) and LOR (right) of protoplasts isolated from leaves ofsfr4 mutant and wild-type seedlings. ○, Protoplasts from NA plants (control); ●, protoplasts from plants subjected to conventional cold acclimation; ⧫, protoplasts from plants that underwent Suc supplementation at low temperature in the dark (400 mm Suc, 2°C, 7 d). The difference in survival after the conventional freeze/thaw and the freeze/hypertonic thaw treatment is attributable to the incidence of EIL, and the difference in 100% survival and survival after the freeze/hypertonic thaw treatment is attributable to the incidence of LOR.sd of data was within ± 3.5%.
- Fig. 3.
Effect of Suc treatment on survival of protoplasts isolated from leaves of sfr4 mutant and wild-type seedlings. ○, Control (before Suc treatment); ●, after Suc treatment. For comparison, survival of protoplasts isolated after the conventional cold acclimation for 7 d (data were taken from Fig. 1) is included in the figure ⧫.
- Fig. 4.
Determination of the effect of the Suc treatment on freezing tolerance of protoplasts isolated from leaves of wild-type seedlings at daily intervals. Survival (upper), the incidence of EIL (middle), and the incidence of LOR (lower) are shown. ○, Control (before Suc treatment); ●, Suc treatment for 1 d; ▴, Suc treatment for 2 d; ▾, Suc treatment for 3 d; ▪, Suc treatment for 4 d; ⧫, Suc treatment for 5 d; black hexagon, Suc treatment for 7 d. For comparison, freezing tolerance of protoplasts isolated after the conventional cold acclimation for 7 d (white hexagon with hair, taken from Figs. 1 and 2) is included in the figure.
- Fig. 5.
Determination of the effect of the Suc treatment on freezing tolerance of protoplasts isolated from leaves ofsfr4 mutant seedlings at daily intervals. Survival (upper), the incidence of EIL (middle), and the incidence of LOR (lower) are shown. For comparison, freezing tolerance of protoplasts isolated after the conventional cold acclimation for 7 d (data were taken from Figs. 1 and 2) is included in the figure. Symbols are the same as those in Figure 4.
