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Research ArticleRESEARCH PAPERS ON SYSTEMS BIOLOGY/GENOMICS/BIOINFORMATICS
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A Resource of Mapped Dissociation Launch Pads for Targeted Insertional Mutagenesis in the Arabidopsis Genome

Paul R. Muskett, Leah Clissold, Adriano Marocco, Patricia S. Springer, Robert Martienssen, Caroline Dean
Paul R. Muskett
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Leah Clissold
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Adriano Marocco
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Patricia S. Springer
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Robert Martienssen
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Caroline Dean
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Published June 2003. DOI: https://doi.org/10.1104/pp.102.016535

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  • © 2003 American Society of Plant Biologists

Abstract

We describe a new resource for targeted insertional mutagenesis in Arabidopsis using a maize (Zea mays) Activator/Dissociation (Ds) two-element system. The two components of the system, T-DNA vectors carrying a Ds launch pad and a stable Activator transposase source, were designed to simplify selection of transposition events and maximize their usefulness. Because Ds elements preferentially transpose to nearby genomic sites, they can be used in targeted mutagenesis of linked genes. To efficiently target all genes throughout the genome, we generated a large population of transgenic Arabidopsis plants containing the Ds launch pad construct, identified lines containing single Ds launch pad inserts, and mapped the positions of Ds launch pads in 89 lines. The integration sites of the Ds launch pads were relatively evenly distributed on all five chromosomes, except for a region of chromosomes 2 and 4 and the centromeric regions. This resource therefore provides access to the majority of the Arabidopsis genome for targeted tagging.

  • Received October 28, 2002.
  • Revised December 21, 2002.
  • Accepted January 26, 2003.
  • Published May 15, 2003.
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A Resource of Mapped Dissociation Launch Pads for Targeted Insertional Mutagenesis in the Arabidopsis Genome
Paul R. Muskett, Leah Clissold, Adriano Marocco, Patricia S. Springer, Robert Martienssen, Caroline Dean
Plant Physiology Jun 2003, 132 (2) 506-516; DOI: 10.1104/pp.102.016535

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A Resource of Mapped Dissociation Launch Pads for Targeted Insertional Mutagenesis in the Arabidopsis Genome
Paul R. Muskett, Leah Clissold, Adriano Marocco, Patricia S. Springer, Robert Martienssen, Caroline Dean
Plant Physiology Jun 2003, 132 (2) 506-516; DOI: 10.1104/pp.102.016535
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Plant Physiology: 132 (2)
Plant Physiology
Vol. 132, Issue 2
Jun 2003
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  • AraCyc: A Biochemical Pathway Database for Arabidopsis
  • Changes in Gene Expression in Arabidopsis Shoots during Phosphate Starvation and the Potential for Developing Smart Plants
  • Annotation of the Arabidopsis Genome
Show more RESEARCH PAPERS ON SYSTEMS BIOLOGY/GENOMICS/BIOINFORMATICS

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