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Research ArticleENVIRONMENTAL STRESS AND ADAPTATION
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Gene Expression Phenotypes of Arabidopsis Associated with Sensitivity to Low Temperatures

Nicholas J. Provart, Pedro Gil, Wenqiong Chen, Bin Han, Hur-Song Chang, Xun Wang, Tong Zhu
Nicholas J. Provart
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Pedro Gil
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Wenqiong Chen
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Bin Han
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Hur-Song Chang
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Xun Wang
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Tong Zhu
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Published June 2003. DOI: https://doi.org/10.1104/pp.103.021261

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    Figure 1.

    Functional classification of genes responding to chilling. The left panel displays the absolute number of genes (represented by probe sets) falling into each category. The right panel shows the results of normalizing the frequency for each category to the frequency of the category in the list of 8,000 genes. Top, Classification of up-regulated genes; Bottom, classification of down-regulated genes.

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    Figure 2.

    Cluster analysis of gene expression of chilling-responsive genes in wild-type and mutant plants. Clusters were based on the various ratios calculated as described in “Materials and Methods.” Red represents an increase in relative expression, whereas green represents a decrease. The order of the genes in the three right panels corresponds to the gene cluster order in the left panel. Panels from left: R, Mutant treated versus mutant untreated; QR, mutant untreated level versus wild-type untreated level; TR, mutant treated versus wild-type treated; RR, mutant treated:untreated/wild-type treated:untreated.

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    Figure 3.

    Genes altered by chilling in common among mutants. Commonalities analysis for up-regulated genes based on pair wise Venn-comparisons among mutants using the TR. The volume of the spheres indicates the number of genes affected using a 2-fold or higher cut-off. The thickness of the lines connecting the spheres denotes the level of commonality, score ranged from 0.1–1.0, between mutants. A red coloring for the line denotes lists with a commonality of 0.2 or less.

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    Figure 4.

    Expression of nuclear-encoded chloroplast-targeted genes. Two hundred and forty-nine nuclear-encoded chloroplast-targeted genes showing 2-fold or more change in wild type, and the corresponding expression levels in mutants. The right column indicate the color codes for functional categories based on the MIPS classification shown in Figure 1.

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    Figure 5.

    Cluster analysis of cold-responsive genes comparing the expression levels in the chilling mutants grown at 22°C and the wild type grown at 22°C. Column Col R represents log2-transformed wild-type treated:wild-type untreated ratio, and 12 rightmost columns correspond to the quiescent levels of these genes (log2-transformed mutant untreated: wild-type untreated ratio) in the mutants.

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Gene Expression Phenotypes of Arabidopsis Associated with Sensitivity to Low Temperatures
Nicholas J. Provart, Pedro Gil, Wenqiong Chen, Bin Han, Hur-Song Chang, Xun Wang, Tong Zhu
Plant Physiology Jun 2003, 132 (2) 893-906; DOI: 10.1104/pp.103.021261

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Gene Expression Phenotypes of Arabidopsis Associated with Sensitivity to Low Temperatures
Nicholas J. Provart, Pedro Gil, Wenqiong Chen, Bin Han, Hur-Song Chang, Xun Wang, Tong Zhu
Plant Physiology Jun 2003, 132 (2) 893-906; DOI: 10.1104/pp.103.021261
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Plant Physiology: 132 (2)
Plant Physiology
Vol. 132, Issue 2
Jun 2003
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