Article Figures & Data
Figures
- Figure 1.
RT-PCR analysis of GhWBC1 (Zhu93) gene expression in developing ovules of cotton cv Xu-142 and fl mutant, collected at 5 DPA. The cotton Histone-3 (GhHIS3) was amplified as a control; in negative control (–), no template DNA was added. The PCR was performed with primers GH1 and GH2.
- Figure 2.
Alignment of amino acid sequences of GhWBC1, Arabidopsis AtWBC11 (At1g17840), a rice putative ABC transporter (AAM92819), human ABCG5 (AAG40003), and fruitfly White protein (P10090). All the proteins aligned here are WBC members of the ABC transporter superfamily. The sequences were aligned with ClustalW (Thompson et al., 1994) using default parameters through EMBnet (http://www.ch.embnet.org/software/ClustalW.html), and the box shade was created by BOXSHADE 3.21 (http://www.ch.embnet.org/software/BOX_form.html).
- Figure 3.
Northern-blot analysis of spatial and temporal pattern of GhWBC1 expression. A, Expression in seedlings (10 d old) and developing ovules (9 DPA); total RNA was isolated from roots (1), hypocotyls (2), cotyledons (3), young true leaves (4), fiber cells (5), wild-type intact ovules (6), and fl ovules (7); 20 μg of total RNA was loaded each lane. B, Expression of GhWBC1 in developing fibers of 5, 9, 15, 20, and 25 DPA, respectively; 1.25 μg of total RNA was loaded each lane. C and D, expression of GhWBC1, E6, and Expansin genes in li mutant and wild-type cotton cv Xu-142 ovules. C, GhWBC1 expression in 1- and 2-DPA ovules; D, GhWBC1, E6, and Expansin expression in 5- and 9-DPA ovules. Ten micrograms of total RNA was loaded each lane.
- Figure 4.
RT-PCR analysis of GhWBC1 expression in different aerial organs of cotton cv Xu-142 and fl mutant. Total RNAs were isolated from hypocotyls (H), cotyledons (C), and young true leaves (L) of the 10-d-old seedlings and from 9-DPA ovules (O) or fibers (F). The PCR was conducted by 27 cycles of PCR amplification with primers GH3 and GH4.
- Figure 5.
Subcellular localization of EGFP-GhWBC1 fusion protein in onion epidermal cells. A, Cell structure under light microscopy. B, Green fluorescence signal of EGFP-GhWBC1 after plasmolysis with 20% (w/v) Suc for 5 min. Plasma membrane and GFP signal shrank together from cell wall.
- Figure 6.
Phenotype of transgenic Arabidopsis plants overexpressing GhWBC1 under a 35S promoter. A, Inflorescence of mature plants of WT (left) and SS-29 transgenic line (right); B, siliques of WT (left) and SS-29 line (right); C, seeds in the 3-d-old siliques of WT (left) and of SS-29 line (right).
- Figure 7.
Silique length and seed number per silique of WT and GhWBC1-transgenic lines of Arabidopsis. A, Length of siliques; B, seed number per silique. LS and SS represent the long (normal) silique lines and the SS lines of transgenic Arabidopsis, respectively. For each line, around 10 siliques were measured.
- Figure 8.
A, RT-PCR analysis of transcripts of GhWBC1 and AtWBC11 in siliques of GhWBC1 transgenic lines of Arabidopsis; higher level of GhWBC1 gene expression was detected in siliques of SS lines. B, RT-PCR analysis of AtWBC11 expression in WT Arabidopsis plants. 1, Roots; 2, leaves; 3, stem; 4, inflorescence; –, negative control without template DNA. The PCR was performed by 30 cycles of amplification. Arabidopsis ACTIN2 (AtACT) was amplified as a control.
