Article Figures & Data
Figures
- Figure 1.
Effect of paclobutrazol (PAC) on photomorphogenesis of dark-grown Arabidopsis wild-type (WT) seedlings. A, Phenotypes of representative 4- and 6-d-old dark-grown seedlings for each treatment (see text). B, Hook angle of 4-d-old seedlings grown in darkness. Measurements are average of 10 to 15 seedlings ± se per treatment. C, Cotyledon angle of 6-d-old seedlings grown in darkness. Measurements are average of 10 to 15 seedlings ± se per treatment. D, Northern-blot analysis of CAB2 and RbcS gene expression in Arabidopsis WT seedlings grown in darkness or in continuous white light for 10 d. Four micrograms of total RNA from light-grown seedlings, and 8 μg of total RNA from dark-grown seedlings was loaded per lane.
- Figure 2.
Effect of Arabidopsis GA biosynthesis and signaling mutants on photomorphogenesis in darkness. A, Phenotypes of representative 5- and 8-d-old dark-grown Arabidopsis GA biosynthesis and signaling mutants. B, Northern-blot analysis of CAB2 and RbcS gene expression in Arabidopsis GA biosynthesis and signaling mutant seedlings grown in darkness or in continuous white light for 10 d. Four micrograms of total RNA from light-grown seedlings and 7 μg of total RNA from dark-grown seedlings were loaded per lane.
- Figure 3.
Interactions between GAs and BRs in the control of Arabidopsis photomorphogenesis in darkness. A, EBR treatment rescues the CAB2 and RbcS WT expression level in 10-d-old WT Arabidopsis seedlings treated with PAC. CAB2 and RcbS mRNA levels were analyzed by northern blot. Four micrograms of total RNA was loaded per lane. B, GA3 does not rescue the molecular phenotype of CAB2 and RbcS in det2 mutants. WT and det2-1 seedlings were grown for 10 d in darkness. CAB2 and RcbS mRNA levels were analyzed by northern blot. Five micrograms of total RNA was loaded per lane.
- Figure 4.
Effects of GA deficiency on photomorphogenesis of pea plants in darkness. A and B, Effect of PAC on photomorphogenesis of pea seedlings in the dark. Seeds of WT cv Alaska were watered with 10 μm PAC and cultured at 22°C in the dark for 6 (A) or 24 (B) d after seeding. GA3 (1 μg) was applied to the seeds before planting, and to the seedlings after germination. C and D, Photomorphogenesis of na seedlings cultured in the dark for 6 (C) or 24 (D) d after seeding and effect of GA3 (GA) and brassinolide (BL) application. E, GA3 dose response on the reversal of photomorphogenesis of na pea seedlings cultured for 15 d in the dark. A single GA3 dose was applied to dry seeds before germination. Numbers mean micrograms of GA3 applied.
- Figure 5.
Effect of PAC and na mutation on RbcS transcript levels in pea. Twenty micrograms of total RNA was loaded per lane. s, Stem; a, apex.
