Article Figures & Data
Figures
- Figure 1.
Kinetics of acquired thermotolerance and freezing tolerance induction in Arabidopsis. A, HS; B, CS. White symbol in B represents 24-h DA at 20°C after 96 h of CS. Error bars represent the 95% confidence interval of the mean. Electrolyte leakage assays were used to determine temperature causing lethal injury for acquired thermotolerance and acquired freezing tolerance.
- Figure 2.
Principal component analysis. Component 1 (differential response to HS and CS) and Component 2 (differential response with respect to the time series) are plotted on the axes. All samples of this investigation are represented. CS samples (black), HS samples (white), untreated control samples (black), DA samples (gray), first experiment (triangles), and second experiment (circles) are shown. Time points are indicated within the graph.
- Figure 3.
Representative HS metabolite responses. A to D demonstrate early; E, intermediate; F, late sustained and transient increase patterns. NA followed by number is an unidentified MST. If a tentative identification is available, MST is characterized with an asterisk.
- Figure 4.
Representative CS metabolite responses. A to C demonstrate early; D to F, intermediate; G and H, late sustained and transient increase patterns. NA followed by number is an unidentified MST. If a tentative identification is available, MST is characterized with an asterisk.
- Figure 5.
Proportionality of metabolite responses to temperature shock. A total of 497 metabolites and MSTs were detected by GC-MS.
Tables
HS-Specific Response Differential Response Metabolites HS CS Metabolites HS CS Uracil I N Phosphoric acid D I d-(−)-Quinic acid I N Glc D I 13 MSTs I N Glc-6-P D I Citramalic acid D N [798; Fru] D I 3 MSTs D N 3 MSTs D I CS-Specific Response Common Response Allantoin N I 2-Ketoglutaric acid I I cis-Aconitic acid N I β-Ala* I I cis-Ferulic acid N I Citric acid* I I cis-Sinapic acid N I Erythritol I I Fru-6-P N I Erythronic acid I I GlcUA N I Fru* I I Glyceric acid-3-P N I Fumaric acid I I l-(+)-Ascorbic acid* N I GABA* I I l-Arg* N I Galactinol* I I l-Cys* N I Galactonic acid I I l-Glu* N I Glycerol* I I l-Gln* N I Gly* I I l-Phe* N I l-Ala* I I l-Pro* N I l-Asn* I I l-Ser* N I l-Glycerol-3-P I I l-Trp* N I l-HomoSer* I I Maleic acid N I l-Ile* I I Man-6-P N I l-Leu* I I Norvaline N I l-Lys* I I O-Acetyl-L-Ser N I l-Met* I I Octadecanoic acid N I l-Thr* I I PyroGlu N I l-Tyr* I I Sorbitol* N I l-Valine* I I trans-Sinapic acid N I Malic acid* I I [497; Gluconic acid-1,4-lactone] N I Maltose* I I [529; Indole-3-acetic acid] N I Melibiose* I I [539; Phe] N I Myoinositol-P I I [612; Pro] N I Orn I I [614; Gln N I Putrescine* I I [640; Putrescine] N I Raffinose* I I [734; l-Asp] N I Ribose I I [861; Glucopyranose] N I SA* I I [889; 1,6-AnhydroGlc] N I Succinic acid I I [949; Glucopyranose] N I Suc* I I 106 MSTs N I Threonic acid I I Isocitric acid N D Threonic acid-1,4-lactone I I Lactic acid N D Trehalose* I I 44 MSTs N D Tyramine I I Differential Response Xyl I I Ara I D [721; Glucaric acid] I I Man I D [732; Pipecolic acid] I I myo-inositol I D [861; Digalactosylglycerol] I I Shikimic acid I D 44 MSTs I I [852; Aminomalonic acid] I D Dehydroascorbic acid dimer D D [708; Ribonic acid] I D Glyceric acid D D [846; Xylitol] I D l-Asp D D 18 MSTs I D 4 MSTs D D I, Increase; D, decrease; N, no significant change in metabolite concentration. Names in brackets precede a match value for an unidentified compound. These names indicate best mass spectral similarity on a scale of 0 to 1,000 (1,000 is identical) to the indicated compound. *, Metabolite showed increase in other environmental stress conditions.
Supplemental Data
Supplemental Tables and Figure
Files in this Data Supplement:
- Supplemental Figure - Supplemental Figure
- Supplemental Tables - Supplemental Tables I-III
