Article Figures & Data
Figures
- Figure 1.
Glycerol-mediated effects on mutants impaired in SA or R gene signaling. A, Microscopy of trypan blue-stained leaves from indicated genotypes treated with water or 50 mm glycerol. SSI2 indicates Col-0 ecotype. B, Endogenous SA and SAG levels in the leaves of indicated 4-week-old soil-grown plants treated with water or glycerol. The values are presented as the mean of three replicates. Error bars represent sd. C, Expression of the PR-1 and PR-2 genes in indicated genotypes. RNA gel-blot analysis was performed on 7 μg of total RNA extracted from 4-week-old soil-grown plants treated with water or glycerol. SSI2 indicates Col-0 ecotype. Ethidium bromide staining of rRNA was used as a loading control. D, Growth of P. parasitica biotype Emco5 on various plant genotypes listed at the left. The Ler and Ws ecotypes were used as the resistant and susceptible controls, respectively. The plants were treated with water (W) or glycerol (G) for 72 h prior to pathogen inoculation and approximately 60 to 75 cotyledons were scored for infection. The shade of each box indicates the severity of infection, based on the number of sporangiophores per cotyledon (see key at the right). Except eds1-1 (Ws background) and nahG (Nö background), all other mutant lines were in Col-0 background. E, Glycerol-induced changes in the 18:1 levels in leaf tissue of 4-week-old plants. Plants were treated with glycerol or water, and samples taken 72 h post treatment were analyzed for FAs using gas chromatography (GC). SSI2 indicates Col-0 ecotype. The values are presented as the mean of six to eight replicates. Error bars represent sd.
- Figure 2.
Morphological, molecular, and biochemical phenotypes of wild-type, ssi2, ssi2 nahG, ssi2 sid2, ssi2 pad4, ssi2 eds1, ssi2 eds5, and ssi2 ndr1 plants. A, Comparison of the morphological phenotypes displayed by the wild-type (SSI2, Nö ecotype), ssi2, and various double-mutant plants in the ssi2 background. B, Microscopy of trypan blue-stained leaves from wild-type (SSI2, Nö ecotype), ssi2, and various double-mutant plants in the ssi2 background. C, Endogenous SA and SAG levels in the leaves of indicated 4-week-old soil-grown plants treated with water or glycerol. The values are presented as the mean of three replicates. Error bars represent sd. D, Expression of the PR-1 and PR-2 genes in indicated genotypes. RNA gel-blot analysis was performed on 7 μg of total RNA extracted from 4-week-old soil-grown plants. SSI2 indicates Nö ecotype. Ethidium bromide staining of rRNA was used as a loading control. E, Growth of P. parasitica biotype Emco5 on various plant genotypes listed at the left. The Ler and Nö ecotypes were used as the resistant and susceptible controls, respectively. The numbers against each box indicate cotyledons scored. The shade of each box indicates the severity of infection, based on the number of sporangiophores per cotyledon (see key at the right). F, Growth of P. syringae on SSI2, ssi2, ssi2 eds1, and ssi2 ndr1. Four leaf discs were harvested from infected leaves at 3 d postinoculation, ground in 10 mm MgCl2, and the bacterial numbers tittered. The bacterial numbers ± sd (n = 4) presented as colony forming units (CFU) per unit leaf area of 25 mm2. The experiment was independently performed twice with similar results.
- Figure 3.
Glycerol-mediated effects on mutants impaired in various FAD steps and double-mutant analysis of ssi2 in different fad backgrounds. A, Comparison of the morphological and cell death phenotypes displayed by the wild-type (SSI2, Col-0 ecotype), fad2, fad3, fad4, fad5, fad6, fad7, and fad7 fad8 plants. The plants were treated with water or glycerol and photographed 3 d post treatment. B, Expression of the PR-1 gene in water- and glycerol-treated fads, wild-type (SSI2, Col-0 ecotype), and act1 plants. RNA gel-blot analysis was performed on 7 μg of total RNA extracted from 4-week-old soil-grown plants. Ethidium bromide staining of rRNA was used as a loading control. C, Endogenous SA levels in the leaves of 4-week-old soil-grown wild-type (Col-0), fad2, fad3, fad4, fad5, fad6, fad7, and fad7 fad8 plants treated with water (W) or glycerol (G). The values are presented as the mean of three replicates. Error bars represent sd. D, Glycerol-induced changes in the 18:1 levels in leaf tissue of 4-week-old plants. The ssi2 gly1 and ssi2 act1 plants were used as controls. Plants were treated with glycerol (G) or water (W), and samples taken 72 h post treatment were analyzed for FAs using GC. The values are presented as the mean of six to eight replicates. Error bars represent sd. E, Comparison of the morphological phenotypes displayed by the ssi2 and various ssi2 fad double- and triple-mutant plants. F, Microscopy of trypan blue-stained leaves from ssi2 and various ssi2 fad double- and triple-mutant plants. G, Expression of the PR-1 and PR-2 genes in wild-type (SSI2, Nö ecotype), ssi2, and various ssi2 fad double- and triple-mutant plants. RNA gel-blot analysis was performed on 7 μg of total RNA extracted from 4-week-old soil-grown plants. Ethidium bromide staining of rRNA was used as a loading control. H, Endogenous SA and SAG levels in the leaves of 4-week-old soil-grown SSI2 (Col-0), ssi2, ssi2 fad7, and ssi2 fad7 fad8 plants. Values are presented as the mean of three replicates. Error bars represent sd. I, Growth of P. syringae on SSI2, ssi2, fad5, ssi2 fad5, fad7, ssi2 fad7, fad7 fad8, and ssi2 fad7 fad8. Four leaf discs were harvested from infected leaves at 3 d postinoculation, ground in 10 mm MgCl2, and the bacterial numbers tittered. The bacterial numbers ± sd (n = 4) presented as colony forming units (CFU) per unit leaf area of 25 mm2. The experiment was independently performed twice with similar results. J, Expression of the PDF1.2 gene in SSI2, ssi2, ssi2 fad7, and ssi2 fad7 fad8 plants in response to 50 μm JA. Samples were harvested 48 h post treatment and analyzed by RNA gel-blot analysis performed on 7 μg of total RNA. Ethidium bromide staining of rRNA was used as a loading control.
- Figure 4.
PA levels and morphological and molecular analyses of ssi2 dgd1 plants. A, PA levels in ssi2 and wild-type (Col-0) and act1 plants treated with water (W) or glycerol (G). The values are presented as the mean of five replicates. Error bars represent sd. According to Student's t test, the difference in PA levels in water- and glycerol-treated samples was not significant (P < 0.05). B, Comparison of the morphological phenotypes displayed by the 4-week-old soil-grown dgd1 and ssi2 dgd1 plants. C, Microscopy of trypan blue-stained leaves from ssi2, dgd1, and ssi2 dgd1 plants. D, Expression of the PR-1 gene in ssi2, dgd1, and ssi2 dgd1 plants. RNA gel-blot analysis was performed on 7 μg of total RNA extracted from 4-week-old soil-grown plants. Ethidium bromide staining of rRNA was used as a loading control. E, Expression of the PR-1 gene and 18:1 levels in water- and glycerol-treated DGD1 (Col-0 ecotype) and dgd1 plants. RNA gel-blot analysis was performed on 7 μg of total RNA extracted from 4-week-old soil-grown plants. Ethidium bromide staining of rRNA was used as a loading control. The 18:1 levels are described as mol% and the values shown are the average of six replicates (±sd).
- Figure 5.
Lipid profile and total lipid content. A, Profile of total lipids extracted from Col-0 and act1 plants treated with water or glycerol. The values are presented as the mean of five replicates. Error bars represent sd. PC, Phosphatidylcholine; PI, phosphatidylinositol; PS, phosphatidylserine. B, Comparison of total lipid content in water- and glycerol-treated SSI2 (Col-0) and act1 plants with that of ssi2. The values are presented as the mean of five replicates. Error bars represent sd.
- Figure 6.
Comparison of glycerol-responsiveness in wild-type, act1, nho1, ssi2 gly1-3, and 35S-ACT1 plants and double-mutant analysis of ssi2 nho1 plants. A, Glycerol-induced changes in the 18:1 levels in leaf tissue of 4-week-old plants. Plants were treated with glycerol (G) or water (W) and samples taken 72 h post treatment were analyzed for 18:1 content using GC. The values shown are an average of six independent replicates. Error bars represent sd. B, Endogenous SA levels in the leaves of 4-week-old soil-grown plants. SSI2 indicates Col-0 ecotype. The values presented are averages of three replicates. Error bars represent sd. C, Expression of the PR-1 gene in water- and glycerol-treated plants. RNA gel-blot analysis was performed on 7 μg of total RNA extracted 72 h after glycerol treatment. The ssi2 gly1-3 plants used in this study were 3 weeks old. Ethidium bromide staining of rRNA was used as a loading control. D, Comparison of the morphological phenotypes displayed by the 16-d-old soil-grown ssi2 and ssi2 nho1 plants. E, Microscopy of trypan blue-stained leaves from ssi2 and various ssi2 nho1 plants. F, Expression of the PR-1 gene in ssi2 and ssi2 nho1 plants. RNA gel-blot analysis was performed on 7 μg of total RNA extracted from 16-d-old soil-grown plants. Ethidium bromide staining of rRNA was used as a loading control.
- Figure 7.
Morphological and molecular phenotypes of ssi2 act1 and various ssi2 act1 fad triple-mutant plants. A, Comparison of the morphological phenotypes displayed by the 4-week-old soil-grown ssi2 act1 and various ssi2 act1 fad triple-mutant plants. B, Microscopy of trypan blue-stained leaves from ssi2 act1 and ssi2 act1 fad triple-mutant plants. C, Expression of the PR-1 gene in wild-type, ssi2, ssi2 act1, and various ssi2 act1 fad triple-mutant plants. RNA gel-blot analysis was performed on 5 μg of total RNA extracted from 4-week-old soil-grown plants. Ethidium bromide staining of rRNA was used as a loading control.
- Figure 8.
A condensed scheme for lipid biosynthesis and glycerol-mediated signaling in Arabidopsis leaves. De novo FA synthesis occurs exclusively in the plastids of all plant cells and leads to the synthesis of palmitic acid (16:0)-ACP and oleic acid (18:1)-ACP. These FAs enter glycerolipid synthesis either via the prokaryotic pathway in the inner envelope of chloroplasts or are exported out of plastids as CoA thioesters to enter the eukaryotic glycerolipid synthesis pathway. Desaturation of stearic acid (18:0)-ACP to 18:1-ACP catalyzed by the SSI2/FAB2-encoded stearoyl-ACP desaturase is one of the key steps in the FA biosynthesis pathway that regulates levels of unsaturated FAs in the cell. The 18:1-ACP generated in this reaction enters the prokaryotic pathway through acylation of G3P and this reaction is catalyzed by the ACT1-encoded G3P acyltransferase. G3P can be made via a cytosolic enzyme GK or via G3Pdh. Dotted line indicates that GK-derived G3P makes a minor contribution to the plastidal G3P pool. Desaturation of 18:1 to 18:2 and 18:3 on membrane glycerolipids (GL) is catalyzed by FAD6 and FAD7/FAD8-encoded desaturases, respectively, that are present on the plastid envelop. Esterification of the CoA group is mediated by acyl-CoA synthetase (ACS). CoA, Coenzyme A; Lyso-PA, acyl-G3P; SL, sulfolipid; DAG, diacylglycerol; DHAP, dihydroxyacetone phosphate.
Tables
- Table I.
Mutant and transgenic lines used in this study and functional properties of the proteins encoded by their wild-type alleles
Mutant Functional Properties References fad2 Endoplasmic reticulum-localized oleoyl-phosphatidylcholine desaturase, desaturates 18:1 to 18:2 Miquel and Browse (1992) fad3 Endoplasmic reticulum-localized linoleoyl-phosphatidylcholine desaturase, desaturates 18:2 to 18:3 Browse et al. (1993) fad4 Desaturates palmitate at sn-2 position of PG in plastids Browse et al. (1985) fad5 Desaturates palmitate at sn-2 position of MGDG in plastids Kunst et al. (1989) fad6 Plastid-localized oleoyl desaturase Falcone et al. (1994) fad7 Plastid-localized linoleate desaturase Iba et al. (1993) fad8 Plastid-localized linoleate desaturase Gibson et al. (1994) nho1 (gli1) Encodes glycerolkinase, converts glycerol to G3P Eastmond (2004); Kang et al. (2003) gly1 Encodes G3Pdh, converts DHAP to G3P Kachroo et al. (2004); Miquel (2003) act1 Encodes G3P acyltransferase, plastid localized, acylates 18:1 on G3P backbone Kunst et al. (1988) dgd1 Plastid-localized galactosyltransferase; involved in synthesis of DGDG from MGDG Dormann et al. (1995) eds1 Encodes lipase-like protein, involved in R gene- and SA-mediated signaling Falk et al. (1999) pad4 Encodes lipase-like protein, involved in SA-mediated signaling Jirage et al. (1999) eds5 Encodes membrane-bound multidrug and toxin-extrusion protein transporter-like protein, involved in SA-mediated signaling Nawrath et al. (2002) ndr1 Encodes plasma membrane-localized protein required for nonrace-specific disease resistance, involved in R gene-mediated signaling Century et al. (1997) sid2 Encodes isochorismate synthase involved in biosynthesis of SA Wildermuth et al. (2001) nahG Bacterial salicylate hydroxylase that converts SA to catachol Yamamotoj et al. (1965) npr1 Encodes a protein containing an ankyrin repeat domain, a key positive regulator of SA signaling and systemic acquired resistance Cao et al. (1997); Ryals et al. (1997) - Table II.
FA composition from leaf tissues of SSI2, ssi2, eds1, ssi2 eds1, ndr1, ssi2 ndr1, eds5, ssi2 eds5, pad4, ssi2 pad4, sid2, ssi2 sid2, nahG, and ssi2 nahG plants
All measurements were made on plants grown at 22°C and data are described as mol% ± sd calculated for a sample size of six.
Genotype 16:0 16:1 16:2 16:3 18:0 18:1 18:2 18:3 Morphology SSI2 14.85 ± 1.41 4.10 ± 0.34 0.99 ± 0.33 16.30 ± 0.33 0.63 ± 0.08 2.31 ± 0.59 13.1 ± 1.55 47.72 ± 1.71 Wild type ssi2 14.81 ± 2.10 2.86 ± 0.44 0.45 ± 0.18 9.0 ± 1.06 15.8 ± 2.82 0.6 ± 0.18 15.68 ± 1.72 40.8 ± 1.45 Stunted eds1 13.73 ± 0.41 4.66 ± 0.12 0.55 ± 0.04 17.95 ± 0.84 0.70 ± 0.04 1.27 ± 0.13 10.41 ± 0.64 50.73 ± 0.96 Wild type-like ssi2 eds1 14.77 ± 2.75 3.85 ± 0.54 0.45 ± 0.1 10.18 ± 1.29 16.9 ± 1.99 0.8 ± 0.15 13.8 ± 2.39 39.25 ± 3.53 ssi2-like ndr1 14.6 ± 0.41 4.65 ± 0.12 1.01 ± 0.1 14.81 ± 0.67 0.54 ± 1.3 2.1 ± 0.48 13.87 ± 1.04 48.42 ± 0.98 Wild type-like ssi2 ndr1 12.34 ± 0.33 2.0 ± 0.1 0.5 ± 0.1 9.84 ± 1.53 18.52 ± 1.3 0.70 ± 0.48 11.16 ± 1.04 45.30 ± 0.98 ssi2-like eds5 14.05 ± 0.36 4.29 ± 0.37 1.17 ± 0.14 16.39 ± 0.74 0.60 ± 0.06 2.39 ± 0.39 14.72 ± 1.05 46.39 ± 0.79 Wild type-like ssi2 eds5 16.68 ± 2.31 3.88 ± 0.96 0.28 ± 0.04 8.88 ± 1.86 14.84 ± 2.59 0.94 ± 0.20 12.66 ± 0.65 41.84 ± 5.00 ssi2-like pad4 14.09 ± 0.88 4.09 ± 0.65 1.18 ± 0.22 15.91 ± 1.59 0.64 ± 0.11 2.27 ± 0.60 15.44 ± 2.29 46.38 ± 1.78 Wild type-like ssi2 pad4 15.16 ± 0.48 3.05 ± 0.72 0.5 ± 0.1 8.62 ± 0.70 17.54 ± 2.36 0.88 ± 0.08 12.25 ± 0.73 42.00 ± 3.95 ssi2-like sid2 13.00 ± 0.36 4.23 ± 0.35 0.84 ± 0.19 17.24 ± 0.92 0.63 ± 0.06 1.42 ± 0.39 12.91 ± 1.90 49.73 ± 1.62 Wild type-like ssi2 sid2 16.68 ± 1.51 5.1 ± 0.75 0.18 ± 0.02 10.68 ± 2.08 17.58 ± 1.80 0.68 ± 0.21 8.68 ± 0.62 40.42 ± 1.19 ssi2-like nahG 14.86 ± 0.90 4.18 ± 0.68 1.14 ± 0.51 16.06 ± 2.30 1.19 ± 0.50 1.75 ± 0.92 14.27 ± 3.29 46.55 ± 3.47 Wild type-like ssi2 nahG 14.60 ± 2.53 3.32 ± 0.31 0.31 ± 0.08 10.0 ± 1.24 18.01 ± 1.07 0.9 ± 0.25 13.68 ± 0.91 39.18 ± 2.81 ssi2-like - Table III.
FA composition from leaf tissues of SSI2, fad2, ssi2 fad2, fad3, ssi2 fad3, fad4, ssi2 fad4, fad5, ssi2 fad5, fad6, ssi2 fad6, fad7, ssi2 fad7, fad7 fad8, and ssi2 fad7 fad8 plants
All measurements were made on plants grown at 22°C and data are described as mol% ± sd calculated for a sample size of six. nd, Not detected.
Genotype 16:0 16:1 16:2 16:3 18:0 18:1 18:2 18:3 Morphology SSI2 15.1 ± 0.47 4.5 ± 0.46 0.9 ± 0.36 16.02 ± 0.77 0.68 ± 0.13 2.9 ± 0.07 12.52 ± 2.36 47.38 ± 2.24 Wild type ssi2 14.12 ± 1.10 3.01 ± 0.65 0.45 ± 0.10 9.4 ± 1.23 16.1 ± 2.22 0.7 ± 0.10 13.68 ± 2.12 42.54 ± 2.47 Stunted fad2 13.7 ± 1.10 4.47 ± 0.42 1.58 ± 0.81 20.58 ± 0.72 0.42 ± 0.13 14.65 ± 2.47 4.18 ± 0.35 40.42 ± 1.43 Wild type-like ssi2 fad2 17.08 ± 1.23 2.66 ± 0.49 0.38 ± 0.08 7.82 ± 1.52 10.83 ± 0.5 19.54 ± 2.02 3.84 ± 0.40 37.85 ± 1.82 ssi2-like fad3 14.58 ± 0.53 4.22 ± 0.32 1.44 ± 0.27 16.4 ± 0.68 0.6 ± 0.12 3.42 ± 0.37 19.84 ± 1.73 39.5 ± 2.25 Wild type-like ssi2 fad3 12.48 ± 1.80 3.66 ± 0.28 0.4 ± 0.1 10.82 ± 2.71 14.72 ± 2.1 1 ± 0.41 20.3 ± 4.89 36.62 ± 5.6 ssi2-like fad4 17.88 ± 0.38 0.7 ± 0.07 1.18 ± 0.15 16.22 ± 0.31 0.6 ± 0 2.38 ± 0.35 13.00 ± 1.43 48.04 ± 1.59 Wild type-like ssi2 fad4 21.02 ± 1.34 –nd – 9.94 ± 0.39 9.82 ± 1.49 0.8 ± 0.1 10.78 ± 1.06 47.64 ± 2.01 ssi2-like fad5 25.64 ± 0.63 4.04 ± 0.61 – – 1.3 ± 0.1 2.5 ± 0.36 14.7 ± 1.94 52.18 ± 1.36 Wild type-like ssi2 fad5 20.22 ± 1.05 1.88 ± 0.14 0.4 ± 0.1 – 21.66 ± 2.0 1.1 ± 0.22 12.44 ± 0.40 42.3 ± 1.67 ssi2-like fad6 14.62 ± 0.38 17.25 ± 1.30 – – 0.88 ± 0.1 22.72 ± 0.39 13.46 ± 0.97 31.07 ± 0.85 Wild type-like ssi2 fad6 17.6 ± 0.62 8.3 ± 0.55 – – 11.36 ± 0.05 11.58 ± 0.87 16.4 ± 0.92 34.76 ± 1.76 Larger than ssi2 fad7 13.42 ± 0.35 4.6 ± 0.45 10.9 ± 0.35 4.1 ± 0.64 0.84 ± 0.15 3.84 ± 0.21 31.2 ± 1.94 31.10 ± 1.50 Wild type-like ssi2 fad7 19.22 ± 1.38 4.27 ± 0.55 11.2 ± 0.8 2.55 ± 0.5 15.47 ± 2.03 1.9 ± 0.42 24.85 ± 1.29 20.39 ± 1.40 Larger than ssi2 fad7 fad8 12.26 ± 0.31 5.36 ± 0.18 16.24 ± 0.78 – 0.52 ± 0.04 3.28 ± 0.63 53.92 ± 0.97 8.42 ± 1.22 Wild type-like ssi2 fad7 fad8 14.93 ± 1.43 4.65 ± 0.50 14.81 ± 0.20 0.51 ± 0.5 14.93 ± 2.61 2.05 ± 0.25 35.9 ± 1.48 12.11 ± 1.74 Larger than ssi2 - Table IV.
FA composition from leaf tissues of SSI2, ssi2, nho1, ssi2 nho1, dgd1, and ssi2 dgd1 plants
All measurements were made on plants grown at 22°C and data are described as mol% ± sd calculated for a sample size of six.
Genotype 16:0 16:1 16:2 16:3 18:0 18:1 18:2 18:3 Morphology SSI2 16.16 ± 0.8 4.24 ± 0.29 1.24 ± 0.08 16.44 ± 0.38 0.92 ± 0.32 2.58 ± 0.08 13.44 ± 0.77 44.98 ± 0.89 Wild type ssi2 14.62 ± 2.13 3.12 ± 0.59 0.52 ± 0.18 10.55 ± 0.83 12.97 ± 2.71 0.95 ± 0.31 12.32 ± 2.23 44.95 ± 2.96 Stunted nho1 15.58 ± 0.45 4.04 ± 0.23 1.06 ± 0.05 16.48 ± 0.25 0.76 ± 0.11 2.58 ± 0.38 13.06 ± 0.89 46.44 ± 1.40 Wild type-like ssi2 nho1 17.1 ± 1.66 2.73 ± 0.6 0.18 ± 0.16 10.2 ± 1.1 13.8 ± 2.4 0.95 ± 0.1 9.78 ± 0.45 45.26 ± 2.59 ssi2-like dgd1 18.37 ± 1.55 4.38 ± 0.6 0.48 ± 0.26 5.87 ± 2.37 1.82 ± 1.91 2.5 ± 0.3 12.5 ± 1.82 54.08 ± 3.59 Smaller than wt ssi2 dgd1 18.83 ± 0.97 2.65 ± 0.25 0.25 ± 0.2 4.89 ± 1.24 12.66 ± 1.51 1.12 ± 0.21 11.26 ± 0.70 48.34 ± 2.59 ssi2-like - Table V.
FA composition from leaf tissues of ssi2, act1, ssi2 act1, ssi2 act1 fad2, ssi2 act1 fad3, ssi2 act1 fad5, ssi2 act1 fad6, and ssi2 act1 fad7 plants
All measurements were made on plants grown at 22°C and data are described as mol% ± sd calculated for a sample size of six. nd, Not detected.
Genotype 16:0 16:1 16:2 16:3 18:0 18:1 18:2 18:3 Morphology ssi2 14.81 ± 2.10 2.86 ± 0.44 0.45 ± 0.18 9.0 ± 1.06 15.8 ± 2.82 0.6 ± 0.18 15.68 ± 1.72 40.8 ± 1.45 Stunted act1 12.5 ± 0.71 2.92 ± 0.32 0.2 ± 0.16 0.35 ± 0.12 1.15 ± 0.1 10.7 ± 1.71 14.58 ± 1.27 57.6 ± 2.72 Wild type-like ssi2 act1 9.17 ± 1.30 1.62 ± 0.22 0.45 ± 0.12 –nd 9.5 ± 2.23 7.22 ± 2.2 16.52 ± 1.23 55.52 ± 3.12 Pale wild type-like fad2 13.7 ± 1.10 4.47 ± 0.42 1.58 ± 0.81 20.58 ± 0.72 0.42 ± 0.13 14.65 ± 2.47 4.18 ± 0.35 40.42 ± 1.43 Wild type-like ssi2 act1fad2 4.2 ± 0.23 1.2 ± 0.5 – – 13.2 ± 1.36 40.9 ± 2.08 3.2 ± 0.55 37.3 ± 2.95 Pale wild type-like fad3 14.58 ± 0.53 4.22 ± 0.32 1.44 ± 0.27 16.4 ± 0.68 0.6 ± 0.12 3.42 ± 0.37 19.84 ± 1.73 39.5 ± 2.25 Wild type-like ssi2 act1fad3 8.18 ± 0.82 2 ± 0.45 – – 9.88 ± 1.20 3.32 ± 0.79 24.16 ± 3.6 52.46 ± 4.01 Pale wild type-like fad4 17.88 ± 0.38 0.7 ± 0.07 1.18 ± 0.15 16.22 ± 0.31 0.6 ± 0 2.38 ± 0.35 13.00 ± 1.43 48.04 ± 1.59 Wild type-like ssi2 act1fad4 16.7 ± 0.36 – – – 6.46 ± 0.61 2.54 ± 0.1 22 ± 1.24 52.3 ± 1.23 Pale wild type-like fad5 25.64 ± 0.63 4.04 ± 0.61 – – 1.3 ± 0.1 2.5 ± 0.36 14.7 ± 1.94 52.18 ± 1.36 Wild type-like ssi2 act1fad5 8.16 ± 0.74 1.54 ± 0.25 – – 13.24 ± 0.32 3.38 ± 1.06 17.76 ± 2.04 55.92 ± 1.97 Pale wild type-like fad6 14.62 ± 0.38 17.25 ± 1.30 – – 0.88 ± 0.10 22.72 ± 0.39 13.46 ± 0.97 31.07 ± 0.85 Wild type-like ssi2 act1fad6 13.4 ± 1.2 14.6 ± 0.7 – – 11.5 ± 1.0 18.2 ± 1.2 13.7 ± 2.1 28.6 ± 2.2 Pale wild type-like fad7 13.42 ± 0.35 4.6 ± 0.45 10.9 ± 0.35 4.1 ± 0.64 0.84 ± 0.15 3.84 ± 0.21 31.2 ± 1.94 31.10 ± 1.50 Wild type-like ssi2 act1fad7 11.67 ± 1.84 – – – 16.05 ± 0.44 7.02 ± 0.74 30.27 ± 5.4 34.99 ± 5.0 Pale wild type-like
Supplemental Data
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- Supplemental Data - Supplemental Figure 1
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