Article Figures & Data
Figures
- Figure 1.
Comparison of a homozygous spl mutant ovule with sibling heterozygous ovule, both at floral stage 13. A, spl mutant ovule showing the complete absence of the embryo sac but presence of all maternal cell types. B, Heterozygous spl plant ovule showing mature embryo sac. Abbreviations: cn, central cell nuclei; en, egg cell nucleus; et, endothelium; ii, inner integument; n, nucellus; oi, outer integument; sn, synergid nucleus. Scale bars represent 50 μm.
- Figure 2.
Identified genes expressed during female gametophyte development. The gene sets included in the Venn diagrams were ≥2-fold higher in wild type than in spl with P values of <0.005 and signal values of >60.
- Figure 3.
Distribution and classification of the female gametophyte genes.
- Figure 4.
GUS expression patterns of the promoter∷GUS fusion with female gametophyte genes in early staged ovule. A to D, Ovule of wild-type plant at FG1 (A), FG2 (B), FG3 (C), and FG4 (D). A, The arrow points the nucleus of functional megaspore. B, Each arrow points a micropylar nucleus or chalazal nucleus within embryo sac. C, The two red arrows point the micropylar nuclei or chalazal nucleus, and a black arrow points a large central vacuole (cv). D, Embryo sac has two micropylar nuclei (red arrows), two chalazal nuclei (black arrows), and a large central vacuole (cv). E, The GUS activity of the promoter∷GUS fusion with At1g26795 was detected in embryo sac at FG4. F, GUS expression of the promoter∷GUS fusion with At1g36340 was detected in the closest nucleus to the chalazal end of the embryo sac at FG4. G, GUS activity of the promoter∷GUS fusion with At2g20070 was detected in dividing nuclei of the FG1 and FG2 embryo sac stages under the dark-field microscope. H, GUS expression of the promoter∷GUS fusion with At4g22050 was observed in chalazal end of embryo sac at FG4. I, GUS activity of the promoter∷GUS fusion with At5g40260 was detected in embryo sac at FG2. Scale bars represent 50 μm.
- Figure 5.
GUS expression patterns of the promoter∷GUS fusion with female gametophyte genes in late staged ovule. A to C, Ovule of wild-type plant at FG5 (A), FG6 (B), and FG7 (C). D, GUS expression of the promoter∷GUS fusion with At1g26795 was detected in the embryo sac at FG5. E, GUS activity of the promoter∷GUS fusion with At1g36340 was detected in the antipodal cells at FG7. F and G, GUS expression of the promoter∷GUS fusion with At2g20070 (F) or At4g22050 (G) was observed in the chalazal end of the embryo sac at FG6. H, GUS activity of the promoter∷GUS fusion with At5g40260 was detected in the embryo sac at FG6. Abbreviations: an, antipodal cells; cn, central cell nucleus; en, egg cell nucleus; pn, polar nuclei; sn, synergid nuclei. Scale bars represent 50 μm.
Tables
- Table II.
Identification of embryo sac genes
Genes were selected on the basis of the P value of <0.0050, fold change of >2.0, and signal intensity of >60.0 at wild-type siblings. WT1–3, Mean of signal intensities for arrays 1 to 3 of wild-type siblings. spl1–3, Mean of signal intensities for arrays 1 to 3 of spl mutants. WTd1–d3, Mean of signal intensities for arrays 1 to 3 of emasculated wild-type siblings. spl1–3, d1–d2, Mean of signal intensities for arrays 1 to 3 of nonemasculated spl, and arrays 1 and 2 of emasculated spl.
Affymetrix Codea Gene IDb Description Early Staged Ovule Late Staged Ovule Divisione WT1–3 spl1–3 FCc P Valued WTd1–d3 spl1–3, d1–d2 FCc P Valued Unknown protein 261271_at At1g26795 Self-incompatibility protein related 769 30 25.3 0.000083 761 27 28.2 0.000000 Both 265133_s_at At1g51250 Expressed protein 151 25 6.1 0.001456 539 19 28.2 0.000000 Both 259726_at At1g60985 Expressed protein 98 16 5.9 0.002140 349 16 21.4 0.000003 Both 265762_at At2g01240 Reticulon family protein (RTNLB15) 227 15 15.1 0.000418 148 14 10.3 0.000020 Both 263713_at At2g20595 Expressed protein 278 15 18.9 0.003556 1,047 25 41.4 0.000000 Both 267193_at At2g30900 Expressed protein 97 23 4.3 0.000554 108 20 5.4 0.000016 Both 256719_at At2g34130 CACTA-like transposase family (Ptta/En/Spm) 117 11 10.3 0.000181 114 10 11.8 0.000000 Both 256600_at At3g14850 Expressed protein 261 34 7.6 0.000088 294 35 8.5 0.000000 Both 257889_at At3g17080 Self-incompatibility protein related 187 30 6.2 0.002931 342 19 17.8 0.000000 Both 251698_at At3g56610 Expressed protein 518 16 32.0 0.004682 1,444 31 46.8 0.000000 Both 254257_s_at At4g23350 Expressed protein 104 30 3.5 0.003879 335 22 15.3 0.000000 Both 253164_at At4g35725 Expressed protein 158 15 10.4 0.003044 298 14 21.2 0.000024 Both 250871_at At5g03930 Expressed protein 171 24 7.0 0.001971 106 14 7.5 0.000002 Both 250325_s_at At5g12060 Self-incompatibility protein related 138 41 3.4 0.001255 206 30 6.8 0.000001 Both 249855_at At5g22970 Expressed protein 261 25 10.6 0.003517 1,028 32 31.7 0.000001 Both 249757_at At5g24316 Pro-rich family protein 471 17 27.7 0.001766 1,328 29 45.6 0.000000 Both 249401_at At5g40260 Nodulin MtN3 family protein 566 37 15.2 0.000603 427 30 14.3 0.000004 Both 248284_at At5g52975 Expressed protein 755 81 9.3 0.000353 403 49 8.2 0.000001 Both 262503_at At1g21670 Expressed protein 161 41 4.0 0.000009 64 45 1.4 0.031616 Early 261731_s_at At1g47780 Acyl-protein thioesterase related 84 15 5.6 0.004140 60 13 4.7 0.000003 Early 249375_at At5g40730 Arabinogalactan protein (AGP24) 806 129 6.2 0.000342 661 575 1.1 0.245297 Early 264610_at At1g04645 Self-incompatibility protein related 106 18 5.7 0.157950 793 21 38.1 0.000557 Late 261846_at At1g11540 Expressed protein 145 107 1.4 0.117890 170 85 2.0 0.000012 Late 255908_s_at At1g18010 Expressed protein 128 64 2.0 0.018058 93 45 2.1 0.000005 Late 256079_at At1g20680 Expressed protein 56 14 3.9 0.011798 67 12 5.7 0.000019 Late 263027_at At1g24010 Expressed protein 23 12 1.9 0.256580 96 22 4.3 0.000001 Late 260942_s_at At1g45190 Expressed protein 318 6 49.3 0.007497 1,404 30 47.1 0.000000 Late 265138_at At1g51300 Acyl-protein thioesterase related 192 43 4.4 0.015275 450 49 9.1 0.000000 Late 262314_at At1g70810 C2 domain-containing protein 23 17 1.4 0.182775 65 17 3.8 0.000004 Late 262972_at At1g75620 Glyoxal oxidase related 67 49 1.4 0.205253 133 38 3.5 0.000079 Late 264297_at At1g78710 Expressed protein 120 62 1.9 0.005001 144 57 2.5 0.000001 Late 267241_at At2g02490 Hydroxyproline-rich glycoprotein family protein 45 4 10.6 0.052466 395 11 37.0 0.000186 Late 267218_at At2g02515 Expressed protein 163 39 4.2 0.007206 838 34 24.8 0.000001 Late 265517_at At2g06090 Self-incompatibility protein related 254 14 17.8 0.013973 1,109 23 47.4 0.000000 Late 264590_at At2g17710 Expressed protein 144 194 0.7 0.361347 841 394 2.1 0.000010 Late 263518_at At2g21655 Expressed protein 271 17 15.8 0.015412 946 25 37.6 0.000001 Late 265674_at At2g32190 Expressed protein 100 61 1.6 0.072973 198 70 2.8 0.000267 Late 265670_s_at At2g32210 Expressed protein 155 158 1.0 0.888298 213 76 2.8 0.000186 Late 265245_at At2g43060 Expressed protein 39 45 0.9 0.564913 131 62 2.1 0.000340 Late 259107_at At3g05460 Sporozoite surface protein related 403 43 9.4 0.008348 1,415 46 31.1 0.000069 Late 258130_at At3g24510 Expressed protein 486 10 48.6 0.012987 2,033 38 53.8 0.000000 Late 252253_at At3g49300 Pro-rich family protein 251 42 6.0 0.007754 626 32 19.3 0.000044 Late 251606_at At3g57840 Self-incompatibility protein related 109 39 2.8 0.058391 468 38 12.4 0.000005 Late 255207_at At4g07515 Expressed protein 453 23 19.8 0.008275 859 32 27.0 0.000000 Late 245424_at At4g17505 Expressed protein 157 55 2.8 0.005099 312 33 9.4 0.000003 Late 254494_at At4g20050 Expressed protein 125 85 1.5 0.069310 230 59 3.9 0.000001 Late 254001_at At4g26260 Expressed protein 174 62 2.8 0.067591 149 25 5.9 0.000073 Late 253724_at At4g29285 Expressed protein 91 12 7.3 0.029036 647 16 39.5 0.000000 Late 253656_at At4g30090 Expressed protein 101 85 1.2 0.276358 76 37 2.1 0.000137 Late 253401_at At4g32870 Expressed protein 88 41 2.1 0.040870 165 39 4.3 0.000012 Late 246641_s_at At5g34885 Expressed protein 216 21 10.3 0.025229 631 27 23.5 0.000000 Late 249179_at At5g42955 Expressed protein 266 14 19.3 0.014861 882 23 38.0 0.000000 Late 248892_at At5g46300 Expressed protein 62 5 11.8 0.014887 124 5 23.1 0.000001 Late Central intermediary metabolism 259786_at At1g29660 GDSL-motif lipase/hydrolase family protein 289 84 3.4 0.003166 879 425 2.1 0.000131 Both 260124_at At1g36340 Ubiquitin-conjugating enzyme, E2 584 38 15.3 0.001661 467 34 13.8 0.000002 Both 245672_at At1g56710 Glycoside hydrolase family 28 protein 218 85 2.6 0.004220 235 94 2.5 0.000298 Both 257442_at At2g28680 Cupin family protein 316 47 6.8 0.001424 303 33 9.2 0.000008 Both 267408_at At2g34890 CTP synthase, putative 152 25 6.1 0.000651 67 16 4.2 0.000011 Both 257243_at At3g24230 Pectate lyase family protein 332 59 5.7 0.003792 198 63 3.2 0.000001 Both 258763_s_at At3g30540 (1-4)-β-Mannan endohydrolase family 125 8 15.9 0.002254 148 9 17.4 0.000000 Both 252342_at At3g48950 Glycoside hydrolase family 28 protein 348 30 11.6 0.001576 603 26 23.3 0.000000 Both 248925_at At5g45910 GDSL-motif lipase/hydrolase-like protein 279 14 20.0 0.001613 1,367 26 53.5 0.000000 Both 247228_at At5g65140 Trehalose-6-phosphate phosphatase 305 38 8.0 0.000647 246 92 2.7 0.000044 Both 264147_at At1g02200 CER1 protein 206 85 2.4 0.000205 40 39 1.0 0.799875 Early 264146_at At1g02205 CER1 protein, At1g02200 650 279 2.3 0.000021 219 225 1.0 0.820363 Early 259703_at At1g77790 Glycosyl hydrolase family 17 protein 113 16 7.0 0.000428 54 11 4.8 0.000000 Early 267202_s_at At2g31030 Oxysterol-binding family protein 74 10 7.3 0.000864 27 7 3.8 0.000005 Early 260611_at At2g43670 Glycosyl hydrolase family 17 protein 132 53 2.5 0.002326 129 75 1.7 0.000212 Early 252320_at At3g48580 Xyloglucan:xyloglucosyl transferase, putative 743 315 2.4 0.003795 1,176 766 1.5 0.004917 Early 250082_at At5g17200 Glycoside hydrolase family 28 protein 71 17 4.2 0.002910 20 11 1.8 0.000578 Early 260947_at At1g06020 PfkB-type carbohydrate kinase family protein 58 40 1.5 0.010463 75 29 2.6 0.000134 Late 259391_s_at At1g06350 Fatty acid desaturase family protein 163 98 1.7 0.342723 196 88 2.2 0.000376 Late 255956_at At1g22015 Galactosyltransferase family protein 158 43 3.7 0.007113 274 34 8.0 0.000000 Late 245792_at At1g32100 Pinoresinol-lariciresinol reductase, putative 66 66 1.0 0.993453 184 68 2.7 0.000127 Late 245794_at At1g32170 Xyloglucan:xyloglucosyl transferase, putative 59 49 1.2 0.363717 101 47 2.1 0.000150 Late 256211_at At1g50960 Gibberellin 20-oxidase related 42 19 2.2 0.015340 75 14 5.4 0.000000 Late 260333_at At1g70500 Polygalacturonase, putative/pectinase, putative 581 27 21.7 0.007959 674 42 16.0 0.000001 Late 260066_at At1g73610 GDSL-motif lipase/hydrolase family protein 69 24 2.9 0.035163 163 20 8.0 0.000056 Late 260259_at At1g74300 Esterase/lipase/thioesterase family protein 60 57 1.1 0.690243 105 47 2.2 0.000095 Late 265331_at At2g18420 Gibberellin-responsive protein, putative 27 25 1.1 0.777953 73 18 4.1 0.000139 Late 267607_s_at At2g26740 Epoxide hydrolase, soluble (sEH) 138 137 1.0 0.977676 205 87 2.4 0.000242 Late 267337_at At2g39980 Transferase family protein 93 46 2.0 0.015251 69 30 2.3 0.000006 Late 260559_at At2g43860 Polygalacturonase, putative/pectinase, putative 113 38 3.0 0.005920 69 30 2.3 0.000032 Late 258767_at At3g10890 (1-4)-β-Mannan endohydrolase, putative 100 16 6.3 0.013753 698 20 35.1 0.000002 Late 258151_at At3g18080 Glycosyl hydrolase family 1 protein 199 194 1.0 0.899935 384 124 3.1 0.000003 Late 257065_at At3g18220 Phosphatidic acid phosphatase family protein 45 29 1.5 0.034020 63 22 2.8 0.000003 Late 251491_at At3g59480 PfkB-type carbohydrate kinase family protein 549 56 9.9 0.032782 598 210 2.8 0.001882 Late 255550_at At4g01970 Galactinol-raffinose galactosyltransferase, putative 127 41 3.1 0.006794 226 83 2.7 0.000170 Late 245349_at At4g16690 Esterase/lipase/thioesterase family protein 75 40 1.9 0.193063 112 53 2.1 0.000368 Late 254609_at At4g18970 GDSL-motif lipase/hydrolase family protein 461 508 0.9 0.189942 703 309 2.3 0.000085 Late 246498_at At5g16230 Acyl-[acyl-carrier-protein] desaturase, putative 34 26 1.3 0.219929 84 23 3.6 0.001229 Late 249983_at At5g18470 Curculin-like (mannose-binding) lectin family protein 44 35 1.2 0.486888 106 48 2.2 0.001507 Late 246774_at At5g27530 Glycoside hydrolase family 28 protein 108 46 2.4 0.012277 146 31 4.8 0.000001 Late 249474_s_at At5g39190 Germin-like protein (GER2) 14 12 1.2 0.500732 99 13 7.6 0.000004 Late 248812_at At5g47330 Palmitoyl protein thioesterase family protein 63 78 0.8 0.143704 203 43 4.7 0.000004 Late 248791_at At5g47350 Palmitoyl protein thioesterase family protein 212 65 3.3 0.040644 1,167 291 4.0 0.000003 Late Hypothetical protein 260318_at At1g63960 Hypothetical protein 62 20 3.2 0.002924 72 20 3.5 0.000014 Both 265579_at At2g20070 Hypothetical protein 661 48 13.8 0.000041 487 36 13.4 0.000000 Both 257434_at At2g21740 Hypothetical protein 128 25 5.0 0.001238 301 20 15.3 0.000000 Both 252753_at At3g43500 Hypothetical protein 80 20 4.0 0.002002 131 20 6.6 0.000002 Both 246859_at At5g25950 Hypothetical protein 70 13 5.4 0.000045 131 12 10.9 0.000000 Both 248225_at At5g53740 Hypothetical protein 168 72 2.3 0.000851 111 52 2.1 0.000065 Both 266706_at At2g03320 Hypothetical protein 97 28 3.5 0.001116 47 17 2.8 0.000175 Early 246866_at At5g25960 Hypothetical protein 131 11 11.7 0.000000 41 9 4.8 0.000020 Early 248396_at At5g52130 Hypothetical protein 111 21 5.4 0.000609 47 15 3.2 0.000216 Early 257468_at At1g47470 Hypothetical protein 147 13 11.2 0.029273 1,010 25 40.1 0.000000 Late 261313_at At1g52970 Hypothetical protein 484 49 9.9 0.007905 1,881 59 32.1 0.000000 Late 259944_at At1g71470 Hypothetical protein 72 60 1.2 0.213121 71 33 2.1 0.000104 Late 263895_at At2g21920 Hypothetical protein 107 26 4.2 0.007542 73 17 4.2 0.000906 Late 258798_at At3g04540 Hypothetical protein 202 55 3.7 0.087047 1,542 50 30.9 0.000000 Late 256773_at At3g13630 Hypothetical protein 31 28 1.1 0.715677 60 27 2.2 0.000839 Late 251607_at At3g57850 Hypothetical protein 73 11 6.6 0.036056 370 13 29.4 0.000050 Late 255029_x_at At4g09470 Hypothetical protein 139 8 17.2 0.012714 687 16 43.5 0.000003 Late 255804_at At4g10220 Hypothetical protein 120 31 3.9 0.009652 410 34 12.0 0.000003 Late 254692_at At4g17860 Hypothetical protein 60 35 1.7 0.028983 107 28 3.9 0.000008 Late 246472_at At5g17130 Hypothetical protein 80 83 1.0 0.853233 75 34 2.2 0.000487 Late 249157_at At5g43510 Hypothetical protein 356 13 27.3 0.012178 588 29 20.1 0.000001 Late 247245_at At5g64720 Hypothetical protein 105 15 6.9 0.010392 322 13 24.5 0.000000 Late Detoxification/stress response 264001_at At2g22420 Peroxidase 17 (PER17) 791 121 6.5 0.003718 725 120 6.1 0.000007 Both 265264_at At2g42930 Glycosyl hydrolase family protein 17 346 55 6.3 0.003492 285 34 8.5 0.000007 Both 254000_at At4g26250 Galactinol synthase induced by water stress 89 18 4.8 0.003892 65 15 4.2 0.000004 Both 252951_at At4g38700 Disease resistance-responsive family protein 248 109 2.3 0.004228 706 115 6.2 0.000000 Both 247857_at At5g58400 Peroxidase, putative 1,053 240 4.4 0.000004 473 161 2.9 0.000023 Both 261410_at At1g07610 Metallothionein-like protein 1C (MT-1C) 333 159 2.1 0.000832 238 170 1.4 0.168590 Early 266743_at At2g02990 Ribonuclease 1 (RNS1) 67 23 2.9 0.001354 140 100 1.4 0.201557 Early 263026_at At1g24000 Bet v I allergen family protein 43 15 2.9 0.266278 250 19 12.9 0.000001 Late 265920_s_at At2g15120 Pseudogene, disease-resistance family protein 38 33 1.1 0.299880 103 23 4.6 0.000933 Late 266562_at At2g23970 Defense-related protein, putative 68 60 1.1 0.479766 194 38 5.1 0.000000 Late 267138_s_at At2g38210 Ethylene-responsive protein, putative 181 86 2.1 0.091609 246 76 3.2 0.001233 Late 266169_at At2g38900 Ser protease inhibitor 40 37 1.1 0.867313 343 105 3.3 0.000002 Late 260557_at At2g43610 Glycoside hydrolase family 19 protein 94 49 1.9 0.003261 76 33 2.3 0.000002 Late 258791_at At3g04720 Hevein-like protein (HEL) 35 35 1.0 0.937369 91 34 2.6 0.003414 Late 258172_at At3g21620 Early responsive to dehydration protein related 60 24 2.5 0.000597 68 21 3.2 0.000002 Late 254098_at At4g25100 Superoxide dismutase (Fe), chloroplast (SODB) 688 313 2.2 0.060518 76 14 5.6 0.000220 Late 253655_at At4g30070 Plant defensin-fusion protein, putative 99 39 2.5 0.007716 167 23 7.4 0.000001 Late 250200_at At5g14130 Peroxidase, putative 94 67 1.4 0.229669 106 41 2.6 0.000012 Late 250083_at At5g17220 Glutathione S-transferase-like protein 99 93 1.1 0.870900 537 235 2.3 0.000020 Late 249560_at At5g38330 Plant defensin-fusion protein, putative 280 26 11.0 0.008435 1,140 29 39.5 0.000000 Late 249527_at At5g38710 Pro oxidase, putative 70 18 3.9 0.006176 74 15 4.8 0.000019 Late Cell structure organization 260573_at At2g47280 Pectinesterase family protein 227 36 6.4 0.000454 139 25 5.5 0.000000 Both 257878_at At3g17150 Invertase/pectin methylesterase inhibitor family 399 26 15.3 0.004375 1,121 38 29.2 0.000000 Both 258438_at At3g17230 Invertase/pectin methylesterase inhibitor family 434 30 14.4 0.000924 758 30 24.8 0.000000 Both 251748_at At3g55680 Invertase/pectin methylesterase inhibitor family 162 51 3.2 0.000901 112 39 2.8 0.000002 Both 255699_at At4g00190 Putative pectinesterase 86 31 2.8 0.001537 90 24 3.7 0.000001 Both 248823_s_at At5g46960 Invertase/pectin methylesterase inhibitor family 270 8 35.1 0.002890 519 13 39.4 0.000003 Both 247246_at At5g64620 Invertase/pectin methylesterase inhibitor family 747 338 2.2 0.000298 870 425 2.0 0.000000 Both 260802_at At1g78400 Glycoside hydrolase family 28 protein 201 23 8.8 0.000001 30 18 1.7 0.001391 Early 258764_at At3g10720 Pectinesterase, putative 415 205 2.0 0.001974 112 77 1.5 0.008263 Early 253725_at At4g29340 Profilin 3 (PRO3) (PFN3) 63 27 2.3 0.000446 26 23 1.1 0.244366 Early 264500_at At1g09370 Pectinesterase inhibitor domain-containing protein 61 10 6.0 0.123949 594 14 43.8 0.000012 Late 259613_at At1g48010 Invertase/pectin methylesterase inhibitor family 38 36 1.1 0.597625 105 29 3.6 0.000000 Late 262083_at At1g56100 Pectinesterase inhibitor domain-containing protein 128 53 2.4 0.444629 2,622 746 3.5 0.000012 Late 245656_at At1g56620 Pectinesterase inhibitor domain-containing protein 50 26 1.9 0.044275 252 19 13.3 0.000000 Late 257679_at At3g20470 Pseudogene, Gly-rich protein 117 99 1.2 0.700308 578 267 2.2 0.001050 Late 245371_at At4g15750 Invertase/pectin methylesterase inhibitor family 200 63 3.2 0.067454 1,378 440 3.1 0.000004 Late 249962_at At5g18990 Pectinesterase family protein 39 19 2.0 0.028245 82 15 5.5 0.000041 Late 247377_at At5g63180 Pectate lyase family protein 175 125 1.4 0.147262 410 192 2.1 0.000518 Late Transport 260319_at At1g63950 Heavy-metal-associated domain-containing protein 310 28 10.9 0.002905 148 19 7.9 0.000001 Both 259757_at At1g77510 Protein disulfide isomerase, putative 1,163 447 2.6 0.000344 943 367 2.6 0.000170 Both 258760_at At3g10780 Emp24/gp25L/p24 family protein 435 94 4.6 0.001770 400 75 5.4 0.000000 Both 245892_at At5g09370 Protease inhibitor/seed storage/lipid transfer protein 384 57 6.8 0.004630 1,326 108 12.3 0.000002 Both 263765_at At2g21540 SEC14 cytosolic factor, putative 135 64 2.1 0.000985 64 42 1.5 0.002042 Early 249346_at At5g40780 Lys- and His-specific transporter, putative 783 170 4.6 0.002599 877 624 1.4 0.054790 Early 264520_at At1g10010 Amino acid permease, putative 91 35 2.6 0.005939 167 34 4.8 0.000000 Late 265002_at At1g24400 Lys- and His-specific transporter 156 41 3.8 0.024933 311 142 2.2 0.000005 Late 259580_at At1g28030 Oxidoreductase, 2OG-Fe(II) oxygenase family protein 52 44 1.2 0.276557 95 32 3.0 0.000024 Late 265064_at At1g61630 Equilibrative nucleoside transporter, putative (ENT7) 70 40 1.7 0.013220 67 29 2.3 0.000179 Late 259844_at At1g73560 Protease inhibitor/seed storage/lipid transfer protein 49 22 2.2 0.005234 91 19 4.9 0.000122 Late 264482_at At1g77210 Sugar transporter, putative 73 65 1.1 0.601658 118 55 2.1 0.000012 Late 257366_s_at At2g03040 Transmembrane protein related 159 39 4.1 0.005632 184 22 8.3 0.000008 Late 266276_at At2g29330 Tropinone reductase, putative 68 30 2.3 0.007410 68 28 2.4 0.000243 Late 254453_at At4g21120 Amino acid permease family protein 211 64 3.3 0.007991 259 88 2.9 0.000000 Late 246887_at At5g26250 Sugar transporter, putative 159 61 2.6 0.015475 146 37 4.0 0.000013 Late 248275_at At5g53520 Oligopeptide transporter OPT family protein 43 32 1.3 0.204722 80 20 3.9 0.000002 Late 248019_at At5g56480 Protease inhibitor/seed storage/lipid transfer protein 61 50 1.2 0.206709 111 38 2.9 0.000015 Late Protein degradation 256486_at At1g31450 Aspartyl protease family protein 263 18 14.8 0.001311 607 17 35.1 0.000000 Both 245738_at At1g44130 Nucellin protein, putative 117 10 12.3 0.002988 94 8 11.2 0.000067 Both 259368_at At1g69100 Aspartyl protease family protein 546 13 42.6 0.003756 952 33 29.1 0.000000 Both 252499_s_at At3g46840 Subtilase family protein 389 34 11.6 0.000398 443 26 17.2 0.000001 Both 245589_at At4g15040 Subtilase family protein 171 23 7.3 0.000154 66 17 4.0 0.000055 Both 254336_at At4g22050 Aspartyl protease family protein 681 37 18.3 0.000096 714 67 10.6 0.000000 Both 246684_at At5g33340 Aspartyl protease family protein 98 25 4.0 0.000228 189 21 9.1 0.000001 Both 247798_at At5g58830 Subtilase family protein 73 15 5.0 0.002673 235 13 17.7 0.000000 Both 254237_at At4g23520 Cys proteinase, putative 67 28 2.4 0.000243 41 22 1.9 0.000254 Early 247697_at At5g59810 Subtilase family protein 92 43 2.1 0.002288 56 44 1.3 0.003872 Early 264067_x_at At2g28010 Aspartyl protease family protein 33 19 1.7 0.039844 78 13 5.9 0.000004 Late 250345_at At5g11940 Subtilase family protein 58 15 3.9 0.005591 154 14 11.4 0.000000 Late Signal transduction 263740_at At2g20660 Rapid alkalinization factor (RALF) family protein 82 33 2.4 0.000758 90 24 3.8 0.000000 Both 245158_at At2g33130 RALF family protein 435 34 12.8 0.000989 437 38 11.4 0.000000 Both 266418_at At2g38750 Annexin 4 (ANN4) 274 62 4.4 0.003825 220 67 3.3 0.000001 Both 251514_at At3g59260 Pirin, putative 202 33 6.1 0.001924 171 30 5.7 0.000002 Both 255489_at At4g02650 Epsin N-terminal homology domain-containing protein 448 22 20.7 0.004357 1,286 32 39.8 0.000000 Both 245177_at At5g12380 Annexin, putative 190 17 11.2 0.000393 215 17 12.8 0.000000 Both 249013_at At5g44700 Leu-rich repeat transmembrane protein kinase 81 31 2.6 0.001303 75 30 2.5 0.000305 Both 261285_at At1g35720 Annexin 1 (ANN1) 168 122 1.4 0.273697 393 108 3.6 0.000012 Late 257869_at At3g25160 ER lumen protein retaining receptor family protein 86 51 1.7 0.030431 147 52 2.8 0.000020 Late 250090_at At5g17330 Glutamate decarboxylase 1 (GAD 1) 195 21 9.2 0.005864 222 25 9.0 0.000000 Late Energy metabolism 259268_at At3g01070 Plastocyanin-like domain-containing protein 79 28 2.8 0.002166 126 26 4.8 0.000001 Both 253634_at At4g30590 Plastocyanin-like domain-containing protein 705 56 12.6 0.000057 500 41 12.3 0.000005 Both 252897_at At4g39490 Cytochrome P450 family protein, At4g38480 62 19 3.4 0.004614 134 18 7.5 0.000000 Both 248236_at At5g53870 Plastocyanin-like domain-containing protein 1,236 60 20.7 0.000394 653 165 4.0 0.000009 Both 262528_at At1g17260 ATPase 10, plasma membrane type, putative 45 26 1.7 0.385722 344 102 3.4 0.000002 Late 261396_at At1g79800 Plastocyanin-like domain-containing protein 49 40 1.2 0.236124 69 31 2.2 0.000064 Late 266563_at At2g23990 Plastocyanin-like domain-containing protein 195 47 4.2 0.011017 953 41 23.3 0.000015 Late 255690_at At4g00360 Cytochrome P450, putative 49 23 2.1 0.018309 79 29 2.7 0.000026 Late 254489_at At4g20800 FAD-binding domain-containing protein 47 11 4.4 0.027803 121 11 11.3 0.000000 Late Transcriptional regulation 267528_at At2g45650 MADS-box protein (AGL6) 245 59 4.2 0.002904 359 170 2.1 0.000048 Both 249338_at At5g41090 No apical meristem (NAM) family protein 166 18 9.1 0.000764 77 13 5.8 0.000000 Both 260212_at At1g74480 RWP-RK domain-containing protein 55 36 1.5 0.071151 65 29 2.2 0.001510 Late 266969_at At2g39540 Gibberellin-regulated family protein 41 38 1.1 0.593532 179 32 5.6 0.000003 Late 254619_at At4g18770 Myb family transcription factor (MYB98) 109 59 1.8 0.063530 110 41 2.7 0.000002 Late 251114_at At5g01380 Expressed protein 25 26 1.0 0.855505 62 23 2.7 0.000211 Late 248240_at At5g53950 No apical meristem (NAM) family protein 224 201 1.1 0.399544 269 110 2.4 0.000013 Late Secondary metabolism 260386_at At1g74010 Strictosidine synthase family protein 110 21 5.1 0.001313 368 22 16.4 0.000010 Both 264401_at At1g61720 Dihydroflavonol 4-reductase family (BAN) 54 45 1.2 0.693582 535 176 3.0 0.000017 Late 260335_at At1g74000 Strictosidine synthase family protein 144 72 2.0 0.005947 104 39 2.7 0.000038 Late 254283_s_at At4g22870 Leucoanthocyanidin dioxygenase, putative 81 85 1.0 0.880918 416 183 2.3 0.000118 Late 249215_at At5g42800 Dihydroflavonol 4-reductase 31 26 1.2 0.546725 301 84 3.6 0.000030 Late Plant development/organogenesis 262113_at At1g02820 Late embryogenesis abundant 3 family protein 82 33 2.5 0.000057 76 27 2.8 0.000001 Both 262659_at At1g14240 Nucleoside phosphatase family protein 80 25 3.3 0.000911 92 16 5.8 0.000000 Both 262549_at At1g31290 PAZ domain-containing protein 140 99 1.4 0.096043 169 63 2.7 0.000011 Late 252234_at At3g49780 Phytosulfokines 3 (PSK3) 17 9 2.0 0.386279 163 43 3.8 0.000014 Late 251301_at At3g61880 Cytochrome P450, putative 94 51 1.9 0.003809 91 41 2.2 0.000032 Late Protein biosynthesis/DNA catabolism 245883_at At5g09500 40S ribosomal protein S15 (RPS15C) 85 18 4.7 0.002080 54 9 5.9 0.000028 Early 260438_at At1g68290 Bifunctional nuclease, putative 44 28 1.6 0.093917 149 25 5.9 0.000104 Late - Table III.
The genes used the promoter∷GUS fusion analysis
Genes were selected on the basis of the P value of <0.0050, fold change of >2.0, and signal intensity of >60.0 at wild-type siblings. Column headings and footnotes are the same as in Table II.
Affymetrix Codea Gene IDb Description Early Staged Ovule Late Staged Ovule WT1–3 spl1–3 FCc P Valued WTd1–d3 spl1–3, d1–d2 FCc P Valued 261271_at At1g26795 Self-incompatibility protein related 769 30 25.3 0.0000827 761 27 28.2 0.0000000 260124_at At1g36340 Ubiquitin-conjugating enzyme, E2 584 38 15.3 0.0016607 467 34 13.8 0.0000023 265579_at At2g20070 Hypothetical protein 661 48 13.8 0.0000412 487 36 13.4 0.0000000 254336_at At4g22050 Aspartyl protease family protein 681 37 18.3 0.0000964 714 67 10.6 0.0000000 253634_at At4g30590 Plastocyanin-like domain-containing protein 705 56 12.6 0.0000568 500 41 12.3 0.0000050 249401_at At5g40260 Nodulin MtN3 family protein 566 37 15.2 0.0006028 427 30 14.3 0.0000038 - Table IV.
The summary for GUS expressions of six promoter∷GUS fusions in seedlings
Fifteen-day-old seedlings were used. To detect weak GUS activity, we used GUS staining solution without potassium ferricyanide/ferrocyanide.
Gene ID GUS Expression At1g26795 None At1g36340 None At2g20070 None At4g22050 Lateral root, petiole At4g30590 None At5g40260 None except occasional trichome
Supplemental Data
Supplemental Figures and Tables and Array Data
Files in this Data Supplement:
- Supplemental Data - Array Data
- Supplemental Data - Supplemental Figures and Tables
Analysis of the Female Gametophyte Transcriptome of Arabidopsis by Comparative Expression Profiling
