Skip to main content

Main menu

  • For Authors
    • Submit a Manuscript
    • Instructions for Authors
  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
    • Focus Collections
    • Classics Collection
    • Upcoming Focus Issues
  • Advertisers
  • About
    • About the Journal
    • Editorial Board and Staff
  • Subscribers
  • Librarians
  • More
    • Alerts
    • Contact Us
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Plant Cell Teaching Tools
    • ASPB
    • Plantae

User menu

  • My alerts
  • Log in

Search

  • Advanced search
Plant Physiology
  • Other Publications
    • Plant Physiology
    • The Plant Cell
    • Plant Direct
    • The Arabidopsis Book
    • Plant Cell Teaching Tools
    • ASPB
    • Plantae
  • My alerts
  • Log in
Plant Physiology

Advanced Search

  • For Authors
    • Submit a Manuscript
    • Instructions for Authors
  • Home
  • Content
    • Current Issue
    • Archive
    • Preview Papers
    • Focus Collections
    • Classics Collection
    • Upcoming Focus Issues
  • Advertisers
  • About
    • About the Journal
    • Editorial Board and Staff
  • Subscribers
  • Librarians
  • More
    • Alerts
    • Contact Us
  • Follow plantphysiol on Twitter
  • Visit plantphysiol on Facebook
  • Visit Plantae
Research ArticleENVIRONMENTAL STRESS AND ADAPTATION TO STRESS
Open Access

Core Genome Responses Involved in Acclimation to High Temperature

Jane Larkindale, Elizabeth Vierling
Jane Larkindale
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Elizabeth Vierling
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site

Published February 2008. DOI: https://doi.org/10.1104/pp.107.112060

  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Article Figures & Data

Figures

  • Tables
  • Additional Files
  • Figure 1.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 1.

    Heat treatments for comparison of acclimated and nonacclimated plants. Shaded arrows represent sampling times and their designations. All samples except unheated, G Acc, and S Acc2 were taken 30 min after the previous temperature transition. G Acc and S Acc2 were taken at the end of acclimation prior to the shift to 45°C.

  • Figure 2.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 2.

    Thermotolerance of plants given different heat treatments. Percent survival of 7-d-old seedlings acclimated (G and S) or not (D) as shown in Figure 1 and then treated at 45°C for the indicated time. A, Appearance of plants at the time of scoring for viability after the indicated heat treatments. B, Graph showing numerical data. Error bars represent sd from three replicate experiments. [See online article for color version of this figure.]

  • Figure 3.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 3.

    Large numbers of transcripts are increased or decreased by heat treatment. Number of transcripts increased (A) or decreased (B) at the indicated sampling time points as defined in Figure 1. Bars are divided to show classes of transcripts with 2- to 5-fold or >5-fold change in level and to show different absolute expression levels (<500, 500–5,000, >5,000 AU).

  • Figure 4.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 4.

    Comparison of transcripts increased by different heat treatments. A, Dendrogram showing relative similarity between different samples. Total array data were clustered using Euclidean distance and average linkage in BRB array tools. G, S, and D sample branches are each shown with differently shaded lines. B to D, Venn diagrams showing the intersections of total numbers of transcripts increased in different samples. B, Acclimation period samples. C, 45°C samples. D, Recovery samples.

  • Figure 5.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 5.

    Summary of gene cluster data. This summary shows some of the bioinformatics data for the clusters discussed in the text. Clusters in column 1 are grouped based on cis-element analysis and show related expression behavior. The transcript accumulation pattern graphed in column 2 is for the cluster indicated in bold in column 1. These graphs show the average log fold-change (base 2) in transcript level at the time points indicated (as in Fig. 1) versus the unheated control. Dark gray bars, S acclimation; light gray bars, G acclimation (three time points; Acc2 includes the G Acc time point); white bars, D treatment (two time points). Error bars represent sd. The complete summary of gene clusters with expression graphs can be found in Supplemental Table S5.

  • Figure 6.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 6.

    Regulation of Pro metabolism is important for heat tolerance. A, Pro oxidase (At3g30775) and P5CS (At3g55610) transcript levels for the different heat treatments. B, Levels of Pro in plants given the following treatments: unheated, S45, G45, and D45 (as in Fig. 1). Data are averages of five biological replicates, error bars represent sd. C, Survival of 7-d-old S- or G-acclimated plants stressed at 45°C for 120 or 180 min, respectively. Wild type (Col-0) and two Pro oxidase T-DNA insertion lines (Supplemental Fig. S4) are compared. [See online article for color version of this figure.]

Tables

  • Figures
  • Additional Files
    • View popup
    Table I.

    Mutants defective in acquired thermotolerance

    Homozygous T-DNA insertion mutants, backcrossed once to wild-type Col-0, were tested for acquired thermotolerance as 7-d-old light-grown seedlings after either S acclimation and 120 min at 45°C (7 d [step] column) or gradual acclimation and 150 min at 45°C (7 d [grad] column). Assays were repeated a minimum of three times with at least 12 seedlings and values are expressed as seedling viability as a percent of wild type. For genes for which two insertion mutants were obtained, values for the two lines are indicated. The maximum sd was ±20%, and all values are significantly different from wild type, with a P value ≥ 0.5. Documentation of the homozygosity of the mutants and absence of full-length RNA is provided in Supplemental Figure S5. Ten other mutants shown to be homozygous but to not have a heat stress phenotype were HSFB1 At4g36990; expressed protein At5g67600; LTI78 At5g52310; DREB2A At5g05410; Fer1 At5g01600; immunophilin At4g25340; stress-induced protein At4g12400; ROF1 At3g25230; galactinol synthase At2g47180; and HSFA2 At2g26150. These mutants were not backcrossed to wild type because of the absence of phenotype.

    cis-ElementsClusterDescriptionAccession7 d (Step)7 d (Grad)Max Fold Induction
    %
    HSE45Hsp101 (hot1-3)At1g7431000106
    HSE7APX2 (two insertions)At3g0964030/2020/20744
    58HSFA7aAt3g51910203025
    25NF-X1At1g10170203012
    63APro oxidase (two insertions)At3g3077520/2040/407
    HSE42SGT1aAt4g2357040308
    HSE41Hsp110 (HSP70-15)At1g79920406013
    42Choline kinaseAt1g74320604010
    DRE25Thaumatin (two insertions)At4g3601060/6060/609

Additional Files

  • Figures
  • Tables
  • Supplemental Data

    Supplemental Tables and Figures

    Files in this Data Supplement:

    • Supplemental Data - Supplemental Figure 1
    • Supplemental Data - Supplemental Figure 2
    • Supplemental Data - Supplemental Figure 3
    • Supplemental Data - Supplemental Figure 4
    • Supplemental Data - Supplemental Figure 5
    • Supplemental Data - Supplemental Figure 6
    • Supplemental Data - Supplemental Data Legends
    • Supplemental Data - Supplemental Figure 7
    • Supplemental Data - Supplemental Table I
    • Supplemental Data - Supplemental Table II
    • Supplemental Data - Supplemental Table III
    • Supplemental Data - Supplemental Table IV
    • Supplemental Data - Supplemental Table V
    • Supplemental Data - Supplemental Table VI
PreviousNext
Back to top

Table of Contents

Print
Download PDF
Email Article

Thank you for your interest in spreading the word on Plant Physiology.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Core Genome Responses Involved in Acclimation to High Temperature
(Your Name) has sent you a message from Plant Physiology
(Your Name) thought you would like to see the Plant Physiology web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Core Genome Responses Involved in Acclimation to High Temperature
Jane Larkindale, Elizabeth Vierling
Plant Physiology Feb 2008, 146 (2) 748-761; DOI: 10.1104/pp.107.112060

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Request Permissions
Share
Core Genome Responses Involved in Acclimation to High Temperature
Jane Larkindale, Elizabeth Vierling
Plant Physiology Feb 2008, 146 (2) 748-761; DOI: 10.1104/pp.107.112060
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • RESULTS
    • DISCUSSION
    • MATERIALS AND METHODS
    • Acknowledgments
    • Footnotes
    • LITERATURE CITED
  • Figures & Data
  • Info & Metrics
  • PDF

In this issue

Plant Physiology: 146 (2)
Plant Physiology
Vol. 146, Issue 2
February 2008
  • Table of Contents
  • Table of Contents (PDF)
  • About the Cover
  • Index by author
  • Advertising (PDF)
  • Back Matter (PDF)
  • Ed Board (PDF)
  • Front Matter (PDF)
View this article with LENS

More in this TOC Section

  • A Comparative Study of Iron Uptake Mechanisms in Marine Microalgae: Iron Binding at the Cell Surface Is a Critical Step
  • Knockdown of a Rice Stelar Nitrate Transporter Alters Long-Distance Translocation But Not Root Influx
  • Deciphering Systemic Wound Responses of the Pumpkin Extrafascicular Phloem by Metabolomics and Stable Isotope-Coded Protein Labeling
Show more ENVIRONMENTAL STRESS AND ADAPTATION TO STRESS

Similar Articles

Our Content

  • Home
  • Current Issue
  • Plant Physiology Preview
  • Archive
  • Focus Collections
  • Classic Collections
  • The Plant Cell
  • Plant Direct
  • Plantae
  • ASPB

For Authors

  • Instructions
  • Submit a Manuscript
  • Editorial Board and Staff
  • Policies
  • Recognizing our Authors

For Reviewers

  • Instructions
  • Journal Miles
  • Policies

Other Services

  • Permissions
  • Librarian resources
  • Advertise in our journals
  • Alerts
  • RSS Feeds

Copyright © 2021 by The American Society of Plant Biologists

Powered by HighWire