Article Figures & Data
Figures
- Figure 1.
Heat treatments for comparison of acclimated and nonacclimated plants. Shaded arrows represent sampling times and their designations. All samples except unheated, G Acc, and S Acc2 were taken 30 min after the previous temperature transition. G Acc and S Acc2 were taken at the end of acclimation prior to the shift to 45°C.
- Figure 2.
Thermotolerance of plants given different heat treatments. Percent survival of 7-d-old seedlings acclimated (G and S) or not (D) as shown in Figure 1 and then treated at 45°C for the indicated time. A, Appearance of plants at the time of scoring for viability after the indicated heat treatments. B, Graph showing numerical data. Error bars represent sd from three replicate experiments. [See online article for color version of this figure.]
- Figure 3.
Large numbers of transcripts are increased or decreased by heat treatment. Number of transcripts increased (A) or decreased (B) at the indicated sampling time points as defined in Figure 1. Bars are divided to show classes of transcripts with 2- to 5-fold or >5-fold change in level and to show different absolute expression levels (<500, 500–5,000, >5,000 AU).
- Figure 4.
Comparison of transcripts increased by different heat treatments. A, Dendrogram showing relative similarity between different samples. Total array data were clustered using Euclidean distance and average linkage in BRB array tools. G, S, and D sample branches are each shown with differently shaded lines. B to D, Venn diagrams showing the intersections of total numbers of transcripts increased in different samples. B, Acclimation period samples. C, 45°C samples. D, Recovery samples.
- Figure 5.
Summary of gene cluster data. This summary shows some of the bioinformatics data for the clusters discussed in the text. Clusters in column 1 are grouped based on cis-element analysis and show related expression behavior. The transcript accumulation pattern graphed in column 2 is for the cluster indicated in bold in column 1. These graphs show the average log fold-change (base 2) in transcript level at the time points indicated (as in Fig. 1) versus the unheated control. Dark gray bars, S acclimation; light gray bars, G acclimation (three time points; Acc2 includes the G Acc time point); white bars, D treatment (two time points). Error bars represent sd. The complete summary of gene clusters with expression graphs can be found in Supplemental Table S5.
- Figure 6.
Regulation of Pro metabolism is important for heat tolerance. A, Pro oxidase (At3g30775) and P5CS (At3g55610) transcript levels for the different heat treatments. B, Levels of Pro in plants given the following treatments: unheated, S45, G45, and D45 (as in Fig. 1). Data are averages of five biological replicates, error bars represent sd. C, Survival of 7-d-old S- or G-acclimated plants stressed at 45°C for 120 or 180 min, respectively. Wild type (Col-0) and two Pro oxidase T-DNA insertion lines (Supplemental Fig. S4) are compared. [See online article for color version of this figure.]
Tables
- Table I.
Mutants defective in acquired thermotolerance
Homozygous T-DNA insertion mutants, backcrossed once to wild-type Col-0, were tested for acquired thermotolerance as 7-d-old light-grown seedlings after either S acclimation and 120 min at 45°C (7 d [step] column) or gradual acclimation and 150 min at 45°C (7 d [grad] column). Assays were repeated a minimum of three times with at least 12 seedlings and values are expressed as seedling viability as a percent of wild type. For genes for which two insertion mutants were obtained, values for the two lines are indicated. The maximum sd was ±20%, and all values are significantly different from wild type, with a P value ≥ 0.5. Documentation of the homozygosity of the mutants and absence of full-length RNA is provided in Supplemental Figure S5. Ten other mutants shown to be homozygous but to not have a heat stress phenotype were HSFB1 At4g36990; expressed protein At5g67600; LTI78 At5g52310; DREB2A At5g05410; Fer1 At5g01600; immunophilin At4g25340; stress-induced protein At4g12400; ROF1 At3g25230; galactinol synthase At2g47180; and HSFA2 At2g26150. These mutants were not backcrossed to wild type because of the absence of phenotype.
cis-Elements Cluster Description Accession 7 d (Step) 7 d (Grad) Max Fold Induction % HSE 45 Hsp101 (hot1-3) At1g74310 0 0 106 HSE 7 APX2 (two insertions) At3g09640 30/20 20/20 744 58 HSFA7a At3g51910 20 30 25 25 NF-X1 At1g10170 20 30 12 63A Pro oxidase (two insertions) At3g30775 20/20 40/40 7 HSE 42 SGT1a At4g23570 40 30 8 HSE 41 Hsp110 (HSP70-15) At1g79920 40 60 13 42 Choline kinase At1g74320 60 40 10 DRE 25 Thaumatin (two insertions) At4g36010 60/60 60/60 9
Supplemental Data
Supplemental Tables and Figures
Files in this Data Supplement:
- Supplemental Data - Supplemental Figure 1
- Supplemental Data - Supplemental Figure 2
- Supplemental Data - Supplemental Figure 3
- Supplemental Data - Supplemental Figure 4
- Supplemental Data - Supplemental Figure 5
- Supplemental Data - Supplemental Figure 6
- Supplemental Data - Supplemental Data Legends
- Supplemental Data - Supplemental Figure 7
- Supplemental Data - Supplemental Table I
- Supplemental Data - Supplemental Table II
- Supplemental Data - Supplemental Table III
- Supplemental Data - Supplemental Table IV
- Supplemental Data - Supplemental Table V
- Supplemental Data - Supplemental Table VI
