Article Figures & Data
Figures
- Figure 1.
The small GTPase regulatory cycle. RAS-related GTPases cycle between an active GTP-bound and an inactive GDP-bound state. GEFs activate these small GTPases, which in turn interact with specific effectors to mediate downstream pathways. GAPs stimulate the inherent GTPase activity of these small G proteins, accelerating the inactivation of their regulatory activity.
- Figure 2.
A schematic illustration for the RAB- and ARF-mediated pathways in plant cells known to date. Phragmoplast and polarized outgrowth are depicted as examples of cellular structures that require high levels of polarized secretory activities for their formation. SV, Secretory vesicles; MVE/PVC, multivesiculated endosome/prevacuolar compartment; RE, recycling endosome.
- Figure 3.
Some model cell systems for the studies of regulatory small GTPases for vesicular trafficking. A, Localization of various GFP-labeled RAB GTPases in transiently transformed Arabidopsis protoplasts. B and C, Localization of NtRAB5 to endosomal compartments in tobacco epidermal cells. B, GFP-NtRAB5 (green) colocalizes on yellow punctate structures with the endocytic marker FM4-64 (red) in epidermal cells of CaMV35S-GFP-NtRab5 transformed tobacco seedlings. C, CFP-NtRAB5 (red) colocalizes with a GFP-labeled auxin efflux protein AtPIN1 (green) known to be recycled through the endosomes (see Geldner and Jürgens, 2006) on yellow punctate structures. The insets in B and C are enlarged from the structures indicated by arrows and arrowheads. The tobacco epidermal cell was transiently cotransformed by agroinfiltration (see Batoko et al., 2000). D, Colocalization of a GFP-NtRAB5 (green) with a CFP-labeled NtRAB11b (red) in an elongating pollen tube showing the polarized concentration of Rab11-labeled transport vesicles in the apex and the localization of Rab5-labeled endosomes in the subtending cytoplasm (B–D; T. Andreyeva and A.Y. Cheung, unpublished data).
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