Article Figures & Data
Figures
- Figure 1.
Dynamics of OsSCAMP1-YFP in cell plate formation during cytokinesis in transgenic BY-2 cells. Shown is a continuous series of time-lapse confocal images of OsSCAMP1-YFP signals collected from a single transgenic tobacco BY-2 cell during cell plate formation. Bar = 50 μm. [See online article for color version of this figure.]
- Figure 2.
OsSCAMP1-YFP largely colocalizes with internalized endosomal marker FM4-64 in the cell plate during cytokinesis in transgenic BY-2 cells. Tobacco BY-2 cells expressing OsSCAMP1-YFP were allowed to take up FM4-64 for 30 min, followed by confocal image collection on cells in various stages of cytokinesis from A to D. DIC, Differential interference contrast. Bar = 50 μm.
- Figure 3.
GONST1-YFP and GFP-BP-80 are largely separated from the internalized endosomal marker FM4-64 during cell plate formation in transgenic BY-2 cells. Tobacco BY-2 cells expressing either the Golgi marker GONST1-YFP (A and B) or the PVC marker GFP-BP-80 (C and D) were allowed to take up FM4-64 under conditions identical to those used for OsSCAMP1-YFP cells, followed by confocal image collection on cells at various stages of cytokinesis. Bar = 50 μm.
- Figure 4.
Immunogold EM of SCAMP1 during cell plate formation in transgenic and wild-type BY-2 cells. Ultrathin sections prepared from high-pressure frozen/freeze-substituted synchronized transgenic (A–C) or wild type (D) BY-2 cells were labeled with either anti-GFP antibodies to detect OsSCAMP1-YFP (A–C) or anti-SCAMP1 antibodies to detect the endogenous SCAMP1 proteins (D). Arrows point to gold particles labeling the cell plates; arrowheads indicate examples of TGN labeling or TGN vesicle labeling. G, Golgi apparatus; M, MVB. Bars = 500 nm.
- Figure 5.
Immunogold EM of VSRs during cell plate formation in BY-2 cells. Ultrathin sections prepared from high-pressure frozen/freeze-substituted synchronized wild-type BY-2 cells were labeled with anti-VSRat-1 antibodies to detect the endogenous tobacco VSR proteins. Arrows point to gold particles labeling the cell plates. Bars = 500 nm.
Additional Files
Supplemental Data
Supplemental Movie
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- Supplemental Data - Supplemental Movie
